Expression of a recombinant branched chain α-oxo acid dehydrogenase complex E2 (BCOADC-E2) in insect cells and its immunoreactivity to autoimmune sera

Experimental and Molecular Medicine

Volumn 30, Issue 2, 1998, Pages 65-71

  Cha, Sang Hoon ^a  

^a Kangwon National University   (South Korea)

Author keywords

Autoantigen; Branched chain oxo acid dehydrogenase complex (BCOADC); Idiopathic cardiomyopathy (IDCM); Insect cells; Primary biliary cirrhosis (PBC)

Indexed keywords

2 OXOISOVALERATE DEHYDROGENASE (LIPOAMIDE); AUTOANTIGEN; HYBRID PROTEIN;

ARTICLE; AUTOIMMUNITY; CELL; EXPRESSION VECTOR; GENE EXPRESSION SYSTEM; IMMUNOREACTIVITY; INSECT; MITOCHONDRIAL RESPIRATION; NONHUMAN; PRIMARY BILIARY CIRRHOSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; RECOMBINANT DNA TECHNOLOGY;

EID: 0032581227     PISSN: 12263613     EISSN: 20926413     Source Type: Journal    
DOI: 10.1038/emm.1998.10     Document Type: Article

References (2)

1. Ansari, A. A., Wang, Y. C., Danner, D. J., Gravanis, M. B., Mayne, A., Neckelmann N., Sell K. W. and Herskowitz A. (1991) Abnormal expression of histocomaptibility and mitochondrial antigens by cardiac tissue from patients with myocarditis and dilated cardiomopathy. Am. J. Pathol. 139: 337-348
2. Ansari, A. A., Neckelmann, N., Villinger, F., Leung, P., Danner, D. J., Brar, S. S., Zhao, S., Gravanis, M. B., Mayne, A., Gershwin, M. E. and Herskowitz, A. (1994) Epitope mapping of the branched α-keto acid dehydrogenase dihydrolipoyl transacylase (BCKD-E2) protein that reacts with sera from patients with idiopathic dilated cardiomyopathy. J. Immunol. 153: 4754-4765