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Volumn 39, Issue 18, 1998, Pages 2721-2724

Shikonin: A geranyl diphosphate-derived plant hemiterpenoid formed via the mevalonate pathway

Author keywords

[No Author keywords available]

Indexed keywords

CARBON 13; GLUCOSE; MEVALONIC ACID; PLANT EXTRACT; SHIKONIN; TERPENOID;

EID: 0032580385     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4039(98)00387-6     Document Type: Article
Times cited : (43)

References (29)
  • 18
    • 0010563869 scopus 로고
    • (Day, P.; Zaitlin, M.; Hollaeder, A. ed.), Academic Press, Orlando
    • (b) Tabata, M.; Fujita, Y. In: Biotechnology in Plant Science (Day, P.; Zaitlin, M.; Hollaeder, A. ed.), 1985, Academic Press, Orlando, pp. 217-218.
    • (1985) Biotechnology in Plant Science , pp. 217-218
    • Tabata, M.1    Fujita, Y.2
  • 19
    • 0010598130 scopus 로고    scopus 로고
    • note
    • 10 which had been cultured in ordinary M9 medium ,it 25°C on a reciprocal shaker (170 strokes /min) in the dark for 5 days. The cells were cultured under the same condition for further 14 days. The paraffin layer was diluted with 120 ml n-hexane, and the medium was extracted with another 120 ml n-hexane. The combined organic phases were extracted with 100 ml 0.5 N NaOH After washing with 2 × 100 ml n-hexane and incubation at room temperature for 10 min (hydrolysis), the aqueous phase was acidified with 5N HCl to pH 3 and extracted with 2 × 120 ml n-hexane. After evaporation of the solvent, the residue (shikonin) was dissolved in methanol and further purified by HPLC using a RP-18 column (250 × 4 mm, 5 μ) and a gradient of methanol in 1% aqueous HCOOH as solvent system. 5.5 mg labelled shikonin were obtained and subjected to NMR analysis.
  • 21
    • 0010633361 scopus 로고    scopus 로고
    • note
    • 13C coupling observed for C·8 in our feeding experiment suggested a reversion of the assignment of the signals of C-8 and C-5.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.