Identification of Differentially Expressed Genes in Human Hepatoblastoma Cell Line (HepG2) and HBV-X Transfected Hepatoblastoma Cell Line (HepG2-4X)

Molecules and Cells

Volumn 8, Issue 2, 1998, Pages 212-218

  Oh, Su Wan ^a   Kim, Nam Soon ^a   Lee, Young Ik ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

Amidophosphoribosyltransferase; DD PCR; HBV X; Hepatoblastoma; HepG2; HepG2 4X; Mitochondrial ATPase Subunit 6

Indexed keywords

DNA; HEPATITIS B VIRUS X PROTEIN; MESSENGER RNA; RECOMBINANT PROTEIN; TRANSACTIVATOR PROTEIN;

ARTICLE; BIOLOGY; CELL CULTURE; CHEMISTRY; COMPARATIVE STUDY; CYTOLOGY; DNA SEQUENCE; FACTUAL DATABASE; GENE; GENE EXPRESSION; GENE EXPRESSION REGULATION; GENETIC TRANSFECTION; GENETICS; HEPATOBLASTOMA; HUMAN; METABOLISM; METHODOLOGY; MICROSCOPY; NORTHERN BLOTTING; PATHOLOGY; SEQUENCE ALIGNMENT;

BLOTTING, NORTHERN; COMPUTATIONAL BIOLOGY; DATABASES, FACTUAL; DNA, NEOPLASM; GENE EXPRESSION; GENE EXPRESSION REGULATION, NEOPLASTIC; GENES; HEPATOBLASTOMA; HUMANS; MICROSCOPY; RECOMBINANT PROTEINS; RNA, MESSENGER; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; TRANS-ACTIVATORS; TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 0032580025     PISSN: 10168478     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (44)

1. Avantaggiati, M. L., Natoli, G., Balsano, C., Chirillo, P., Artini, M., De Marzio, E., Collepardo, D., and Levrero, M. (1993) The hepatitis B virus (HBV) pX transactivates the c-fos promoter through multiple cis-acting elements. Oncogene 8, 1567-1574.
2. Balsano, C., Avantaggiati, M. L., Natoli, G., De Marizo, E., Elfassi, E., Will, H., and Levrero, M. (1991) in Viral Hepatitis and Liver Disease, Hollinger, F. B, Lemon, S. M. and Margolis H. (eds.) pp. 572-578, Williams and Wilkins, Baltimore.
3. Beasley, R. P., Hwang, L. Y., Lin, C. C., and Chien, C. S. (1981) Hepatocellular carcinoma and hepatitis B virus. A prospective study of 22,707 men in Taiwan. Lancet 2, 1129-1133.
4. Benn, J. and Schneider, R. J. (1994) Hepatitis B virus HBx protein activates Ras-GTP complex formation and establishes a Ras, Raf, MAP kinase signaling cascade. Proc. Natl. Acad. Sci. USA 91, 10350-10354.
5. Buendia, M. A. (1992) Hepatitis B viruses and hepatocellular carcinoma. Adv. Cancer Res. 59, 167-226.
6. Capuano, F., Varone, D., D'Erig, N., Russo, E., Tommasi, S., Montemurro, S., Prete, F., and Papa, S. (1996) Oxidative phosphorylation and F(O)F(1) ATP synthase activity of human hepatocellular carcinoma. Biochem. Mol. Bio. Int. 38, 1013-1022.
7. Cheong, J. H., Yi, M., Lin, Y., and Murakami, S. (1995) Human RPB5, a subunit shared by eukaryotic nuclear RNA polymerases, binds human hepatitis B virus X protein and may play a role in X transactivation. EMBO J. 14, 143-150.
8. Colgrove, R., Simon, G., and Ganem, D. (1989) Transcriptional activation homologous and heterologous genes by the hepatitis B virus X gene in cells permissive for viral replication. J. Virol. 63, 4019-4026.
9. Costello, L. C. and Franklin, R. B. (1994) Bioenergetic theory of prostate malignancy. Prostate 25, 162-166.
10. Cross, J. C., Wen, P., and Rutter, W. (1993) Transactivation by hepatitis B virus X protein is promiscuous and dependent on mitogen-activated cellular serine/threonine kinases. Proc. Natl. Acad. Sci. USA 90, 8078-8082.
11. Kay, A., Mandart, E., Trepo, C., and Galibert, F. (1985) The HBV HBX gene expressed in E. coli is recognised by sera from hepatitis patients. EMBO J. 4, 1287-1292.
12. Kekule, A. S., Lauer, U., Weiss, L., Luber, B., and Hofschneider, P. H. (1993) Hepatitis B virus transactivator HBx uses a tumor promoter signalling pathway. Nature 361, 742-745.
13. Kim, C. M., Koike, K., Saito, I., Miyamura, T., and Jay, G. (1991) HBx gene of hepatitis B virus induces liver cancer in transgenic mice. Nature 351, 317-320.
14. Kim, Y. S. and Kang, H. S. (1984) Cloning and expression of hepatitis B virus gene. Korean Biochemical J. 17, 70-79.
15. Knowles, B. B., Searls, D. B., and Aden, D. P. (1984) Advances in Hepatitis Research, Chisari, F. V. (ed), pp. 196-202. Masson Publishing, New York.
16. Koike, K., Akatsuka, T., and Miyamura, T. (1988) Characterization of hepatitis B virus X gene: in vitro translation of mRNA from COS-1 cells transfected with the X gene. Virolory 163, 233-235.
17. Koike. K, Shirakata, Y., Yaginuma, K., Arii, M., Takada, S., Nakamura, I., Hayashi, Y., Kawada, M., and Kobayashi, M. (1989) Oncogenic potential of hepatitis B virus. Molecular Biology and Medicine 6, 151-160.
18. Lee, Y. I., Bong, Y. S., Poo, H. R., Lee, Y. I., Park, J. K., Oh, S. W., Shon, M. J., and Hyun, S. W. (1998) Establishment and characterization of cell lines constitutively expressing Hepatitis B Virus X-protein (in press).
19. Liang, P. and Pardee, A. B. (1992) Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257, 967-971.
20. Linskens, M. H. K., Teng, J., Andrews, W. H., Enlow, B. E., Satti, S. M., Tonkin, L. A., Funk, W. D., and Villeponteau, B. (1995) Cataloging altered gene expression in young and senescent cells using enhanced differential display. Nucleic Acids Res. 23, 3244-3251.
21. Lucito, R. and Schneider, R. J. (1992) Hepatitis B virus X protein activates transcription factor NF-kappa B without a requirement for protein kinase C. J. Virol. 66, 983-991.
22. Maguire, H. F., Hoeffler, J. P., and Siddiqui, A. (1991) HBV X protein alters the DNA binding specificity of CREB and ATF-2 by protein-protein interactions. Science 252, 842-844.
23. Mane, Y., Mukaida, N., Kuno, K., Akiyama, M., Ikeda N., Matsushima, K., and Murakami, S. (1991) Hepatitis B virus X protein transactivates human interleukin-8 gene through acting on nuclear factor kB and CCAAT/enhancer-binding protein-like cis-elements. J. Biol. Chem. 266, 13759-13763.
24. Maniatis, T., Fritsch, E. F., and Sambrook, J. (1982) Molecular Cloning: A Laboratory Mannual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
25. Menzo, S., Clementi, M., Alfani, E., Bagnarelli, P., Iscovscci, S., Manzin, A., Dandri, M., Natili, G., Levrero, M., and Carloni, G. (1993) Trans-activation of epidermal growth factor receptor gene by the hepatitis B virus X-gene product. Virology 196, 878-882.
26. Meyers, M. L., Vitvitski-Trepo, L. V., Nath, N., and Sninsky, J. J. (1986) Hepatitis B virus polypeptide X: expression in Escherichia coli and identification of specific antibodies in sera from hepatitis B virus-infected humans. J. Virol. 57, 101-109.
27. Murakami, S., Cheong, J. H., Ohno, S., Matsushima, K., and Kaneko, S. (1994) Transactivation of human hepatitis B virus X protein, HBx, operates through a mechanism distinct from protein kinase C and okadaic acid activation pathways. Virology 199, 243-246
28. Natoli, G., Avantaggiati, M. L., Chirillo, P., Costanzo, A., Artini, M., Balsano, C., and Levrero, M. (1994a) Induction of the DNA-binding activity of c-jun/c-fos heterodimers by the hepatitis B virus transactivator pX. Mol. Cell. Biol. 14, 989-998.
29. Natoli, G., Avantaggiati, M. L., Chirillo, P., Puri, P. L., Lanni, A., Balsano, C., and Levrero, M. (1994b) Ras-and Raf-dependent activation of c-jun transcriptional activity by the hepatitis B virus transactivator pX. Oncogene 9, 2837-2843.
30. Ojala, D., Montoya, J., and Attardi, G. (1981) tRNA punctuation model of RNA processing in human mitochondria. Nature 290, 470-474.
31. Papa, S. (1989) Molecular structure and interaction; in Organelles in Eukaryotic cells, Tager, J. M., Azzi, A., Papa, S., and Guerrieri, F. (eds.), pp. 9-26, Plenum Publ. Co., New York and London.
32. Pearson, W. R. and Lipman, D. J. (1988) Improved tools for biological sequence comparison. Proc. Natl. Acad. Sci. USA 85, 2444-2448.
33. Qadri, I., Maquire, H. F., and Siddiqui, A. (1995) Hepatitis B virus transactivator protein X interacts with the TATA-binding protein. Proc. Natl. Acad. Sci. USA 92, 1003-1007.
34. Robinson, W. S. (1994) Molecular events in the pathogenesis of hepadnavirus-associated hepatocellular carcinoma. Amu. Rev. Med. 45, 297-323
35. Rossner, M. T., (1992) Review: hepatitis B virus X-gene product: a promiscuous transcriptional activator. J. Med. Virol. 36, 101-117.
36. Sato, N. and Hagihara, B. (1970) Spectrophotometric analyses of cytochromes in ascites hepatomas of rats and mice. Cancer Res. 30, 2061-2068.
37. Schreiber, J. R., Balcavege, W. X., Morris, H. P., and Pedersen, P. L. (1970) Enzymatic and spectral analysis of cytochrome oxidase in adult and fetal rat liver and Morris hepatoma 3924A. Cancer Res. 30, 2497-2501.
38. Senior, A. E. (1988) The proton-translocating ATPase of Escherichia coli. Physiol. Rev. 68, 177-232.
39. Slagie, B. L., Lee, T.-H., and Butel, J. S. (1992) Hepatitis B virus and hepatocellular carcinoma. Prog. Med. Virol. 39, 167-203.
40. Twu, J. S., Lai, Y., Chen, D. S., and Robbinson, W. S. (1993) Activation of protooncogene c-jun by the X protein of hepatitis B virus. Virology 192, 346-350.
41. Walker, J. E., Luter, R., Dupuis, A., and Runswick, M. J. (1991) Identification of the subunits of F1F0-ATPase from bovine heart mitochondria. Biochemistry 30, 5369-5378.
42. Wattiaux, R. and Wattiaux-de Coninck, S. (1968) Subcellular particles in tumors. I. Intracellular distribution of cytochrome oxidase, glucose-6-phosphatase, catalase, and several acid hydrolases in a transplantable chemically induced hepatoma. Eur. J. Cancer 4, 193-200.
43. White, M. T. and Tewari, K. K. (1973) Structural and functional changes in Novikoff hepatoma mitochondria. Cancer Res. 33, 1645-1653.
44. Williams, J. S. and Andrisani, O. M. (1995) The hepatitis B virus X protein targets the basic region-leucine zipper domain of CREB. Proc. Natl. Acad. Sci. USA 92, 3819-3823.