Visualization and molecular analysis of actin assembly in living cells

Journal of Cell Biology

Volumn 143, Issue 7, 1998, Pages 1919-1930

  Schafer, Dorothy A ^a   Welch, Matthew D ^b   Machesky, Laura M ^c   Bridgman, Paul C ^a   Meyer, Shelley M ^a   Cooper, John A ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

Actin assembly; Arp2 3 complex; Capping protein; Cell motility; Rho family GTPase

Indexed keywords

ACTIN; COFILIN; GREEN FLUORESCENT PROTEIN; GUANOSINE TRIPHOSPHATASE; PHOSPHATIDYLINOSITOL KINASE; PROTEIN; RHO FACTOR; RHODAMINE;

ACTIN FILAMENT; ANIMAL CELL; ARTICLE; CELL MOTILITY; ELECTRON MICROSCOPY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; RAT;

ACTINS; ANIMALS; CDC42 GTP-BINDING PROTEIN, SACCHAROMYCES CEREVISIAE; CELL CYCLE PROTEINS; CELL MEMBRANE; CELL MOVEMENT; FIBROBLASTS; GTP-BINDING PROTEINS; MACROPODIDAE; MICROFILAMENTS; PHOSPHOPROTEIN PHOSPHATASE; PROTEIN KINASE INHIBITORS; RAC GTP-BINDING PROTEINS; RECOMBINANT FUSION PROTEINS; RHOA GTP-BINDING PROTEIN; TETRADECANOYLPHORBOL ACETATE;

ANIMALIA; EUKARYOTA;

EID: 0032576597     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.143.7.1919     Document Type: Article

References (15)

1. Abe, H., T. Obinata, L.S. Minamide, and J.R. Bamburg. 1996. Xenopus laevis actin-depolymerizing factor/cofilin: a phosphorylation-regulated protein essential for development. J. Cell Biol. 132:871-885.
2. Arber, S., F.A. Barbayannis, H. Hanser, C. Schneider, C.A. Stanyon, O. Bernard, and P. Caroni. 1998. Regulation of actin dynamics through phosphorylation of cofilin by LIM-kinase. Nature. 393:805-809.
3. Bretscher, A. 1989. Rapid phosphorylation and reorganization of ezrin and spectrin accompany morphological changes induced in A-431 cells by epidermal growth factor. J. Cell Biol. 108:921-930.
4. Bridgman, P.C., and T.S. Reese. 1984. The structure of cytoplasm in directly frozen cultured cells. I. Filamentous meshworks and the cytoplasmic ground substance. J. Cell Biol. 99:1655-1668.
5. Bubb, M.R., A.M. Senderowicz, E.A. Sausville, K.L. Duncan, and E.D. Korn. 1994. Jasplakinolide, a cytotoxic natural product, induces actin polymerization and competitively inhibits the binding of phalloidin to F-actin. J. Biol. Chem. 269:14869-14871.
6. Bubb, M.R., I. Spector, A.D. Bershadsky, and E.D. Korn. 1995. Swinholide A is a microfilament disrupting marine toxin that stabilizes actin dimers and severs actin filaments. J. Biol. Chem. 270:3463-3466.
7. Carlier, M.F., V. Laurent, J. Santolini, R. Melki, D. Didry, G.X. Xia, Y. Hong, N.H. Chua, and D. Pantaloni. 1997. Actin depolymerizing factor (ADF/Cofilin) enhances the rate of filament turnover-implication in actin-based motility. J. Cell Biol. 136:1307-1322.
8. (36/32), a barbed end actin-capping protein, is a component of the Z-line of skeletal muscle. J. Cell Biol. 105:371-379.
9. Casella, J.F., M.D. Flanagan, and S. Lin. 1981. Cytochalasin D inhibits actin polymerization and induces depolymerization of actin filaments formed during platelet shape change. Nature. 293:302-305.
10. Chakraborty, T., F. Ebel, E. Domann, K. Niebuhr, B. Gerstel, S. Pistor, C.J. Temm-Grove, B.M. Jockusch, M. Reinhard, U. Walter, and et al. 1995. A focal adhesion factor directly linking intracellularly motile Listeria monocytogenes and Listeria ivanovii to the actin-based cytoskeleton of mammalian cells. EMBO (Eur. Mol. Biol. Organ.) J. 14:1314-1321.
11. Coue, M., S.L. Brenner, I. Spector, and E.D. Korn. 1987. Inhibition of actin polymerization by latrunculin A. FEBS (Fed. Eur. Biochem. Soc.) Lett. 213:316-318.
12. Cramer, L.P., and T.J. Mitchison. 1995. Myosin is involved in postmitotic cell spreading. J. Cell Biol. 131:179-189.
13. D'Souza-Schorey, C., R.L. Boshans, M. McDonough, P.D. Stahl, and L. Van Aelst. 1997. A role for POR1, a rac1-interacting protein in ARF6-mediated cytoskeletal rearrangements. EMBO (Eur. Mol. Biol. Organ.) J. 16:5445-5454.
14. DiNubile, M.J., L. Cassimeris, M. Joyce, and S.H. Zigmond. 1995. Actin filament barbed-end capping activity in neutrophil lysates: the role of capping protein-beta 2. Mol. Biol. Cell. 6:1659-1671.
15. Dustin, M.L., M.W. Olszowy, A.D. Holdorf, J. Li, S. Bromley, N. Desai, P. Widder, F. Rosenberger, P.A. van der Merwe, P.M. Allen, and A.S. Shaw. 1998. A novel adaptor protein orchestrates receptor patterning and cytoskeletal polarity in T-cell contacts. Cell. 94:667-677.