Cloning and Sequencing of the ce/A Gene Encoding CMCase of Erwinia carotovora subsp. carotovora LY34

Molecules and Cells

Volumn 8, Issue 1, 1998, Pages 27-35

  Park, Yong Woo ^a   Lim, Sun Tech ^a   Yun, Han Dae ^a  

^a Gyeongsang National University   (South Korea)

Author keywords

celA; CMC SDS PAGE; CMCase; Erwinia carotovora subsp. carotovora

Indexed keywords

CARBOXYMETHYLCELLULASE; CELA ENDOGLUCANASE; CELLULASE; GLYCOSIDASE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENE; CHEMISTRY; DNA SEQUENCE; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PECTOBACTERIUM CAROTOVORUM; PHYLOGENY; RESTRICTION MAPPING; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BASE SEQUENCE; CELLULASE; CLONING, MOLECULAR; GENES, BACTERIAL; GLYCOSIDE HYDROLASES; MOLECULAR SEQUENCE DATA; PECTOBACTERIUM CAROTOVORUM; PHYLOGENY; RESTRICTION MAPPING; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 0032573967     PISSN: 10168478     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Beguin, P. (1990) Molecular biology of cellulose degradation. Annu. Rev. Microbiol. 44, 219-248.
2. Boyer, M. H., Chambost, J. P., Magnan, M., and Cattaneo, J. (1987) Characterization of a new endoglucanase from Erwinia chrysanthemi. Eur. J. Biochem. 162, 311-316.
3. Collmer, A. and Keen, N. T. (1986) The role of pectin enzymes in plant pathogenesis. Annu. Rev. Phytopathol. 24, 383-409.
4. Cooper, V. J. C. and Salmond, G. P. C. (1993) Molecular analysis of the major cellulase (CelV) of Erwinia carotovora: evidence for an evolutionary 'mix-and-match' of enzyme domains. Mol. Gen. Genet. 241, 341-350.
5. Gilkes, N. R., Henrissat, B., Kilburn, D. G., Miller, R. C. Jr., and Warren, R. A. J. (1991) Domains in microbial β-1,4-glucanase: sequence conservation, function, and enzyme families. Microbiol. Rev. 55, 303-315.
6. Guiseppi, A., Cami, B., Aymeric, J. L., and Ball, Y. (1988) Homology between endoglucanase Z of Erwinia chrysanthemi and endoglucanase of Bacillus subtilis and alkalophilic Bacillus. Mol. Microbiol. 2, 1591-1604.
7. Henrissat, B., Claeyssens, M., Tomme, P., Lemesle, L., and Mornon, J. P. (1989) Cellulase families revealed by hydrophobic cluster analysis. Gene 81, 83-95.
8. Hinton, J. C. D., Sidebotham, J. M., Gill, D. R., and Salmond, G. P. C. (1989) Isolation and characterization of transposon-induced mutants of Erwinia carotovora subsp. atroseptica exhibiting reduced virulence. Mol. Microbiol. 3, 1785-1795.
9. Hinton, J. C. D. and Salmond, G. P. C. (1987) Use of TnPhoA to enrich for extracellular enzyme mutants Erwinia carotovora subsp. carotovora. Mol. Microbiol. 1, 381-386.
10. Kotoujansky, A. (1887) Molecular genetics of pathogenesis by soft-rot Erwinia. Annu. Rev. Phytopathol. 25, 405-430.
11. Laemmli, U. K. (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 277, 680-685.
12. Lim, S. T. (1997) Molecular cloning and characterization of cellulose degradation-enzyme in Erwinia carotovora. Ph D. Dissertation. The Graduate School of Gyeongsang National University.
13. Mae, A. R., Heikinheimo, R., and Palva, E. T. (1995) Structure and regulation of the Erwinia carotovora SCC3193 cellulase gene celV1 and the role of cellulase in phytopathogenicity. Mol. Gen. Genet. 247, 17-26.
14. Olsen, O., Thomsen, K. K., Weber, J., Duus, J., Svendsen, I., Wegener, C., and von Wettstein, D. (1996) Transplanting two unique endoglucanase catalytic activities into one multienzyme which forms glucose. Biotechnology 14, 71-76.
15. Perombelon, M. C. K. and Kelman, A. (1980) Ecology of the soft-rot Erwinias. Annu. Rev. Phytopathol. 18, 361-387.
16. Robson, L. M. and Chambliss, G. H. (1987) Endo-β-1,4-glucanase gene of Bacillus subtilis DLG. J. Bacterial. 169, 2017-2025.
17. Saarilahti, H. T., Henrissat, B., and Palva, E. T. (1990) CelS: a novel endoglucanase identified from Erwinia carotovora subsp. carotovora. Gene 90, 9-14.
18. Sambrook, J., Fritsch, E. F., and Maniatis. T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
19. Wood, W. A. and Scott, T. K. (1988) Methods in Enzymology 160, 87-112.
20. Yun, H. D., Lim, S. T., and Kang, K. Y. (1990) Molecular cloning of the cell-wall degrading enzyme in Rhizobium. Kor. J. Appl. Microbiol. Biotech. 18, 203-208.
21. Yun, H. D., Lim, S. T., Chung, M. H., Park, Y. W., Kim, H. K., and Kang, K. Y. (1992) Genomic cloning of the extracellular cellulase gene from Xanthomonas campeatris pv. oryzae. Mol. Cells. 2, 17-22.