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Volumn 282, Issue 5394, 1998, Pages 1721-1724

Control of cyclin ubiquitination by CDK-regulated binding of Hct1 to the anaphase promoting complex

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIN DEPENDENT KINASE; HYDROXYUREA; NOCODAZOLE; UBIQUITIN; UBIQUITIN PROTEIN LIGASE;

EID: 0032573374     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.282.5394.1721     Document Type: Article
Times cited : (450)

References (30)
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    • note
    • Strains were W303 derivatives. The genomic HCT1 open reading frame was tagged with Myc epitopes as described (6). Hct1 is a rare protein. In growing cells, the amount of Myc9-Hct1 was approximately 20-fold lower than that of Cdc23-Myc9 (12). In Fig. 3, A and B, a HA3-HCT1-TRP1 plasmid was integrated at the HCT1 locus.
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    • note
    • We thank R. Mattes, J. Altenbuchner, M. Galova, and M. Shirayama for plasmids and strains and D. Kellogg for antibodies to Clb3. Supported by the Deutsche Forschungsgemeinschaft and the Austrian Industrial Research Promotion Fund.


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