Synthetic approaches to macromolecular models for ion channel proteins

Tetrahedron Letters

Volumn 39, Issue 35, 1998, Pages 6377-6380

  Azam, Farzana ^a   Bladon, Christine M ^a  

^a UNIVERSITY OF KENT   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BROMIDE; ION CHANNEL; MEMBRANE PROTEIN;

ARTICLE; ION TRANSPORT; MACROMOLECULE; MOLECULAR DYNAMICS; PROTEIN SYNTHESIS; STEREOCHEMISTRY;

EID: 0032572884     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4039(98)01313-6     Document Type: Article

References (16)

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10. 5. Atherton, E.; Sheppard, R.C. Solid Phase Peptide Synthesis: A Practical Approach, IRL Press: Oxford, 1989.
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12. 2 and the resulting mixture was stirred at room temperature for 20 min. The precipitated dicyclohexylurea was filtered off and the anhydride solution was immediately added to the resin-bound template.
13. 8. As determined by the Kaiser test: Kaiser, E.; Colescott, R.L.; Bossinger, C.D.; Cook. P.I. Anal. Biochem. 1970, 34, 595-598.
14. 3CN (eluent B). Elution was at a flow rate of 1ml/min and 2.5ml/min for the analytical and the semi-preparative columns respectively. The eluent from the column was monitored at 220 nm.
15. 10. Capillary zone electrophoresis (CZE): analysis carried out on a Beckman P/ACE 5000 series instrument using a 50cm (75μmID) eCAP capillary. The separation was driven-by a 15kV potential and a pH 8.35 buffer (0.4% boric acid , 0.3% sodium borate) and the eluent was monitored at 214nm.
16. 11. Recorded on a VG Tofspec with Matrix Assisted Laser Desorption Time of Flight Instrument (MALDITOF) mass spectrometer using a matrix of α-cyanocinnamic acid. An error of ± 1 in 1000 units is quoted by the manufacturer.