Allelic retrieval: A scheme to facilitate the repeated isolation of a specific segment of the Bordetella pertussis chromosome

Gene

Volumn 208, Issue 2, 1998, Pages 183-189

  Stibitz, Scott ^a  

^a CENTER FOR BIOLOGICS EVALUATION AND RESEARCH   (United States)

Author keywords

Allelic exchange; bvg locus; rpsL; sacB; Suicide vector

Indexed keywords

PLASMID VECTOR;

ALLELE; ARTICLE; BACTERIAL VIRULENCE; BORDETELLA PERTUSSIS; CHROMOSOME; ESCHERICHIA COLI; GENE ISOLATION; GENE MUTATION; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL;

ALLELES; BACTERIAL PROTEINS; BORDETELLA PERTUSSIS; CHROMOSOME MAPPING; CHROMOSOMES, BACTERIAL; CLONING, MOLECULAR; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC TECHNIQUES; MODELS, GENETIC; PLASMIDS; RESTRICTION MAPPING; TRANSCRIPTION FACTORS; VIRULENCE;

EID: 0032570717     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(97)00643-4     Document Type: Article

References (29)

1. Birnboim H.C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1979;1513-1520.
2. Blomfield I.C., Vaughn V., Rest R.F., Eisentstein B.I. Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC101 replicon. Mol. Microbiol. 5:1991;1447-1457.
3. Butterton J.R., Boyko S.A., Calderwood S.B. Use of the Vibrio cholerae irgA gene as a locus for insertion and expression of heterologous antigens in cholera vaccine strains. Vaccine. 11:1993;1327-1335.
4. Cai Y.P., Wolk C.P. Use of a conditionally lethal gene in Anabena sp. strain PCC7120 to select for double recombinants and to entrap insertion sequences. J. Bacteriol. 172:1990;3138-3145.
5. Cianciotto N.P., Long R., Eisenstein B.I., Engleberg N.C. Site-specific mutagenesis in Legionella pneumophila by allelic exchange using counterselectable ColE1 vectors. FEMS Microbiol. Lett. 56:1988;203-208.
6. Copass M., Grandi G., Rappuoli R. Introduction of unmarked mutations in the Helicobacter pylori vacA gene with a sucrose sensitivity marker. Infect. Immun. 65:1997;1949-1952.
7. Fu R., Voodrouw G. Targeted gene-replacement mutagenesis of dcrA, encoding a potential oxygen sensor of the sulfate-reducing bacterium Desulfovibrio vulgaris Hildenborough. Microbiology. 143:1997;1815-1826.
8. Gambello M.J., Iglewski B.H. Cloning and characterization of the Pseudomonas aeruginosa lasR gene, a transcriptional activator of elastase production. J. Bacteriol. 173:1991;3000-3009.
9. Geissler S., Drummond M. A counterselectable pACYC184-based lacZ alpha-complementing plasmid vector with novel multiple cloning sites; construction of chromosomal deletions in Klebsiella pneumoniae. Gene. 136:1993;253-255.
10. Hosted T.J., Baltz R.H. Use of rpsL for dominance selection and gene replacement in Streptomyces roseosporus. J. Bacteriol. 179:1997;180-186.
11. Kamoun S., Tola E., Kamdar H., Kado C.I. Rapid generation of directed and unmarked deletions in Xanthomonas. Mol. Microbiol. 6:1992;809-816.
12. Kaniga K., Delor I., Cornelis G.R. A wide-host-range suicide vector for improving reverse genetics in gram-negative bacteria: inactivation of the blaA gene of Yersinia enterocolitica. Gene. 109:1991;137-141.
13. Merkel T., Stibitz S. Identification of a locus required for the regulation of bvg-repressed genes in Bordetella pertussis. J. Bacteriol. 177:1995;2727-2736.
14. Miller, J., 1972. Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
15. Mobley H.L., Jarvis K.G., Elwood J.P., Whittle D.I., Lockatell C.V., Russell R.G., Johnson D.E., Donnenberg M.S., Warren J.W. Isogenic P-fimbrial deletion mutants of pyelonephritogenic Escherichia coli: the role of alpha Gal (1-4) beta Gal binding in virulence of a wild-type strain. Mol. Microbiol. 10:1993;143-155.
16. Pelicic V., Reyrat J.M., Gicquel B. Positive selection of allelic exchange mutants in Mycobacterium bovis BCG. FEMS Microbiol. Lett. 144:1996;161-166.
17. Quandt J., Hynes M.F. Versatile suicide vectors which allow direct selection for gene replacement in Gram-negative bacteria. Gene. 127:1993;15-21.
18. Ried J.L., Collmer A. An nptI-sacB-sacR cartridge for constructing directed, unmarked mutations in Gram-negative bacteria by marker exchange-eviction mutagenesis. Gene. 57:1987;239-246.
19. Ruvkun A.U., Ausubel F.M. A general method for site-directed mutagenesis in prokaryotes. Nature. 289:1981;85-88.
20. +: a dominant selectable marker for gene replacement in mycobacteria. Mol. Microbiol. 16:1995;991-1000.
21. Schweizer H.P. Allelic exchange in Pseudomonas aeruginosa using novel ColE1-type vectors and a family of cassettes containing a portable oriT and the counter-selectable Bacillus subtilus sacB marker. Mol. Microbiol. 6:1992;1195-1204.
22. Simon R., Priefer U., Puhler A. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Bio/Technology. 1:1983;784-789.
23. Skorupski K., Taylor R. Positive selection vectors for allelic exchange. Gene. 169:1996;47-52.
24. Skrzypek E., Haddix P.L., Plano G.V., Straley S.C. New suicide vector for gene replacement in yersiniae and other Gram-negative bacteria. Plasmid. 29:1993;160-163.
25. Stibitz S., Black W., Falkow S. The construction of a cloning vector designed for gene replacement in Bordetella pertussis. Gene. 50:1986;133-140.
26. Stibitz S. Mutations in the bvgA gene of Bordetella pertussis that differentially affect regulation of virulence determinants. J. Bacteriol. 176:1994;5615-5621.
27. Stibitz S. Use of conditionally counterselectable suicide vectors for allelic exchange. Meth. Enzymol. 235:1994;458-465.
28. Stibitz S., Carbonetti N. Hfr mapping of mutations in Bordetella pertussis that define a locus involved in virulence gene regulation. J. Bacteriol. 176:1994;7260-7266.
29. Wu S.S., Kaiser D. Markerless deletion of pil genes in Myxococcus xanthus generated by counterselection with the Bacillus subtilus sacB gene. J. Bacteriol. 178:1996;5817-5821.