Unusual nonreducing sugar GlcNAcβ(1 mutually implies 1)Manβ formation by β-N-acetylhexosaminidase from Aspergillus oryzae

Tetrahedron Letters

Volumn 39, Issue 52, 1998, Pages 9777-9780

  Křen, Vladimír ^a   Rajnochová, Eva ^a   Huňková, Zdenka ^a   Dvořáková, Jana ^a   Sedmera, Petr ^a  

^a INSTITUTE OF MICROBIOLOGY   (Czech Republic)

Author keywords

[No Author keywords available]

Indexed keywords

4 NITROPHENYL BETA 2 N ACETYLGLUCOSAMINIDE; BETA N ACETYLHEXOSAMINIDASE; GLYCOSYLTRANSFERASE; MANNOSE; N ACETYLGLUCOSAMINE; UNCLASSIFIED DRUG;

ARTICLE; ASPERGILLUS ORYZAE; CARBOHYDRATE SYNTHESIS; ENZYME GLYCOSYLATION; GLYCOSYLATION; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; REACTION ANALYSIS; STRUCTURE ANALYSIS;

EID: 0032564553     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4039(98)02171-6     Document Type: Article

References (17)

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9. 2O), and minor amounts of 2a and 3a.
10. 2-5 column (250 × 10 mm) (GL Sciences Inc., USA); the mobile phase was acetonitrile - water (78 : 22, v/v) at a flow rate 5.0 ml/min, temperature 50 °C; retention times were 12.86 and 14.63 min for 1a and 2a, respectively.
11. 3CO), 58.09 d (C-2′), 63.49 t (C-6′), 64.06 t (C-6), 69.72 d (C-4), 72.66 d (C-4′), 73.40 d (C-2), 75.57 d (C-3), 76.78 d (C-3′), 78.94 d (C-5), 79.34 d (C-5′), 99.57 d (C-1), 100.64 d (C-1′), 177.92 s (C=O).
12. 3CO).
13. 1H NMR of 4b -present as a minor impurity (under 10 %) in the sample of 2b - δ 3.867 (1H, dd, J = 9.5, 9.5, H-4α), 5.449 (1H, dd, J = 9.5, 3.3, H-3α).
14. 3CO), 53.8 d (C-2′α), 62.1 t (C-6′α), 69.5 t (C-6α), 67.8 d (C-4α), 68.4 d (C-4′α), 69.7 d (C-2α), 68.9 d (C-3α), 70.7 d (C-5α), 72.2 d (C-5′α), 72.5 d (C-3′α), 92.5 d (C-1α), 100.8 d (C-1′α). * Signal overlaps.
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