Herpesvirus thymidine kinase transgenes that do not cause male sterility are aberrantly transcribed and translated in the testis

Biochimica et Biophysica Acta - Gene Structure and Expression

Volumn 1442, Issue 1, 1998, Pages 28-38

  Ellison, Aaron R ^a,b   Bishop, John O ^a,b  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b UNIVERSITY OF MARYLAND BALTIMORE COUNTY   (United States)

Author keywords

Alternative transcription initiation; Testis; Thymidine kinase; Tissue specific expression; Transgenic mouse

Indexed keywords

THYMIDINE KINASE;

ANIMAL TISSUE; ARTICLE; GENETIC TRANSCRIPTION; HERPES VIRUS; IMMUNOBLOTTING; MALE; MALE STERILITY; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA TRANSLATION; TESTIS; TRANSCRIPTION INITIATION; TRANSGENE; VIRUS TRANSCRIPTION;

ANIMALS; BASE SEQUENCE; CATTLE; GENE EXPRESSION REGULATION; GENES, REPORTER; INFERTILITY, MALE; MALE; MICE; MICE, TRANSGENIC; MOLECULAR SEQUENCE DATA; PROTEIN BIOSYNTHESIS; TESTIS; THYMIDINE KINASE; TRANSCRIPTION, GENETIC; VIRAL PROTEINS;

ANIMALIA; BOVINAE; HERPES; HERPESVIRIDAE; HUMAN HERPESVIRUS 1; HUMAN HERPESVIRUS 2; MUS MUSCULUS;

EID: 0032561441     PISSN: 01674781     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4781(98)00112-2     Document Type: Article

References (29)

1. Al-Shawi R., Burke J., Jones C.T., Simmons J.P., Bishop J.O. A Mup promoter-thymidine kinase reporter gene shows relaxed tissue-specific expression and confers male sterility upon transgenic mice. Mol. Cell. Biol. 8:1988;4821-4828.
2. Braun R.E., Lo D., Pinkert C.A., Widera G., Flavell R.A., Palmiter R.D., Brinster R.L. Infertility in male transgenic mice: disruption of sperm development by HSV-tk expression in postmeiotic germ cells. Biol. Reprod. 43:1990;684-693.
3. Al-Shawi R., Burke J., Wallace H., Jones C., Harrison S., Buxton D., Maley S., Chandley A., Bishop J.O. The herpes simplex virus type 1 thymidine kinase is expressed in the testes of transgenic mice under the control of a cryptic promoter. Mol. Cell. Biol. 11:1991;4207-4216.
4. Bernier F., Guérin S.L., Ouellet M., Pelletier G., Pothier F. Binding of multiple nuclear proteins from mouse testis to the cryptic promoter of the herpes simplex virus type 1 thymidine kinase gene. Transgenics. 1:1994;225-240.
5. Ellison A.R., Bishop J.O. Initiation of herpes simplex virus thymidine kinase polypeptides. Nucleic Acids Res. 24:1996;2073-2079.
6. Ellison A.R., Wallace H., Al-Shawi R., Bishop J.O. Different transmission rates of herpesvirus thymidine kinase reporter transgenes from founder male parents and male parents of subsequent generations. Mol. Reprod. Dev. 41:1995;425-434.
7. Wallace H., Ledent C., Vassart G., Bishop J., Al-Shawi R. Specific ablation of thyroid follicle cells in adult transgenic mice. Endocrinology. 129:1991;3217-3226.
8. Jamieson A.T., Subak-Sharpe J.H. Biochemical studies on the herpes simplex virus-specified deoxypyrimidine kinase activity. J. Gen. Virol. 24:1974;481-492.
9. Bradford M. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
10. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:1987;156-159.
11. Fourney R.M., Miyakoshi J., Day III R.S., Paterson M.C. Northern blotting: efficient RNA staining and transfer. Bethesda Res. Lab. Focus. 10:1988;5.
12. Feinberg A.P., Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132:1983;6-13.
13. J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular Cloning: a Laboratory Manual, 2nd edn., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.
14. Church G.M., Gilbert W. Genomic sequencing. Proc. Natl. Acad. Sci. USA. 81:1984;1991-1995.
15. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
16. H.A.C. Wallace, Generation and Analysis of an Inducible Thyroxine-deficient Mouse Model, Ph.D. thesis, University of Edinburgh, 1992.
17. Markkula M.A., Hämäläinen T.M., Zhang F., Kim K.E., Maurer R.A., Huhtaniemi I.T. The FSH β-subunit promoter directs the expression of herpes simplex virus type 1 thymidine kinase to the testis of transgenic mice. Mol. Cell. Endocrinol. 96:1993;25-36.
18. Salomon B., Maury S., Loubiere L., Caruso M., Onclercq R., Klatzmann D. A truncated herpes simplex virus thymidine kinase phosphorylates thymidine and nucleoside analogs and does not cause sterility in transgenic mice. Mol. Cell. Biol. 15:1995;5322-5328.
19. Kozak M. The scanning model for translation: an update. J. Cell Biol. 108:1989;229-241.
20. Kozak M. Bifunctional messenger RNAs in eukaryotes. Cell. 47:1986;481-483.
21. Marsden H.S., Haarr L., Preston C.M. Processing of herpes simplex virus proteins and evidence that translation of thymidine kinase mRNA is initiated at three separate AUG codons. J. Virol. 46:1983;434-445.
22. Huttner K.M., Pudney J., Milstone D.S., Ladd D., Seidman J.G. Flagellar and acrosomal abnormalities associated with testicular HSV-tk expression in the mouse. Biol. Reprod. 49:1993;251-261.
23. Haarr L., Flatmark T. Evidence that deletion of coding sequences in the 5′ end of the thymidine kinase gene of herpes simplex virus type 1 affects the stability of the gene products. J. Gen. Virol. 68:1987;2817-2829.
24. Coen D., Irmiere A., Jacobson J., Kerns K. Low levels of HSV1-tk are not limiting for sensitivity to certain antiviral drugs or latency in a mouse model. Virology. 168:1989;221-231.
25. Balzarini J., Bohman C., De Clercq E. Differential mechanism of cytostatic effect of (E)-5(2-bromovinyl, 9-(1,3-dihydroxy-2-propoxymethyl)guanine, and other antiherpetic drugs on tumor cells transfected by the thymidine kinase gene of herpes simplex virus type 1 or type 2. J. Biol. Chem. 268:1993;6332-6337.
26. Balzarini J., Bohman C., Walker R.T., De Clercq E. Comparative cytostatic activity of different antiherpetic drugs against herpes simplex virus thymidine kinase gene-transfected tumor cells. Mol. Pharmacol. 45:1994;1253-1258.
27. Culver K.W., Ram Z., Wallbridge S., Ishii H., Oldfield E.H., Blease R.M. In vivo gene transfer with retroviral vector-producer cells for treatment of experimental brain tumors. Science. 256:1992;1550-1552.
28. Chen S.-H., Shine H.D., Goodman J.C., Grossman R.G., Woo S.L.C. Gene therapy for brain tumors: regression of experimental gliomas by adenovirus-mediated gene transfer in vivo. Proc. Natl. Acad. Sci. USA. 91:1994;3054-3057.
29. Zeiger M.A., Takiyama Y., Bishop J.O., Ellison A.R., Saji M., Levine M.A. Adenoviral infection of thyroid cells: a rationale for gene therapy for metastatic thyroid carcinoma. Surgery. 120:1996;921-925.