Truncations at the NH2 terminus of rhodanese destabilize the enzyme and decrease its heterologous expression

Journal of Biological Chemistry

Volumn 273, Issue 43, 1998, Pages 27841-27847

  Trevino, Richard J ^a   Tsalkova, Tamara ^b   Kramer, Gisela ^b   Hardesty, Boyd ^b   Chirgwin, John M ^a   Horowitz, Paul M ^a  

^a UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

^b UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

THIOSULFATE SULFURTRANSFERASE; PEPTIDE FRAGMENT; RECOMBINANT PROTEIN;

ALPHA HELIX; AMINO TERMINAL SEQUENCE; ARTICLE; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME STABILITY; GENE DELETION; IMMUNOPRECIPITATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PROCESSING; PROTEIN STABILITY; AMINO ACID SEQUENCE; CELL FREE SYSTEM; ENZYME ACTIVE SITE; ESCHERICHIA COLI; GENETICS; METABOLISM; MOLECULAR GENETICS; MUTATION; SERODIAGNOSIS;

AMINO ACID SEQUENCE; CATALYTIC DOMAIN; CELL-FREE SYSTEM; ENZYME STABILITY; ESCHERICHIA COLI; MOLECULAR SEQUENCE DATA; MUTATION; PEPTIDE FRAGMENTS; PRECIPITIN TESTS; PROTEIN FOLDING; RECOMBINANT PROTEINS; SEQUENCE DELETION; THIOSULFATE SULFURTRANSFERASE;

EID: 0032561078     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.273.43.27841     Document Type: Article

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