Monitoring the sizes of denatured ensembles of staphylococcal nuclease proteins: Implications regarding m values, intermediates, and thermodynamics

Biochemistry

Volumn 37, Issue 51, 1998, Pages 18010-18017

  Baskakov, Ilia V ^a   Bolen, D W ^a  

^a University of Texas Medical Branch School of Medicine   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEASE; STAPHYLOCOCCAL NUCLEASE; UNCLASSIFIED DRUG;

ARTICLE; ENZYME DENATURATION; FLUORESCENCE; GEL PERMEATION CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN STRUCTURE; STAPHYLOCOCCUS; THERMODYNAMICS; VALIDATION PROCESS;

ALANINE; AMINO ACID SUBSTITUTION; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CHYMOTRYPSINOGEN; MICROCOCCAL NUCLEASE; MUTAGENESIS, SITE-DIRECTED; PROTEIN DENATURATION; REPRODUCIBILITY OF RESULTS; SPECTROMETRY, FLUORESCENCE; THERMODYNAMICS; THREONINE; TRYPTOPHAN; UREA; VALINE;

EID: 0032558991     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi981849j     Document Type: Article

References (30)

1. Shortle, D., and Meeker, A. K. (1986) Proteins 1, 81-89.
2. Shirley, B. A., Stanssens, P., Steyaert, J., and Pace, C. N. (1989) J. Biol. Chem. 264, 11621-11625.
3. Smith, C. K., Zimei, B., Anderson, K. S., Sturtevant, J. M., Engelman, D. M., and Regan, L. (1996) Protein Sci. 5, 2009-2019.
4. Bowler, B. E., May, K., Zaragoza, T., York, P., A., D., and Caughey, W. S. (1993) Biochemistry 32, 183-190.
5. Carra, J. H., Anderson, E. A., and Privalov, P. L. (1994) Biochemistry 33, 10842-10850.
6. Carra, J. H., and Privalov, P. L. (1995) Biochemistry 34, 2034-2041.
7. Gittis, A. G., Stites, W. E., and Lattman, E. E. (1993) J. Mol. Biol. 232, 718-724.
8. Shortle, D. (1995) in Advances in Protein Chemistry (Anfinsen, C. B., Edsall, J. T., Richards, F. M., & Eisenberg, D. S., Eds.) pp 217-247, Academic Press, New York.
9. Carra, J. H., and Privalov, P. L. (1996) FASEB J. 10, 67-74.
10. Hagel, P., Gerding, J. J. T., Fieggen, W., and Bloemendal, H. (1971) Biochim. Biophys. Acta 243, 366-373.
11. Pace, C. N. (1986) in Enzyme Structure, Part L (Hirs, C. H. W., and Timasheff, S. N., Eds.) pp 266-280, Academic Press, Inc.
12. Shortle, D., and Meeker, A. K. (1989) Biochemistry 28, 936-944.
13. Eftink, M. R., Ionescu, R., Ramsay, G. D., Wong, C.-Y., Wu, J. Q., and Maki, A. H. (1996) Biochemistry 35, 8084-8094.
14. Uversky, V. N. (1993) Biochemistry 32, 13288-13298.
15. Corbett, R. J. T., and Roche, R. S. (1984) Biochemistry 23, 1888-1894.
16. Santoro, M. M., and Bolen, D. W. (1988) Biochemistry 27, 8063-8068.
17. Yao, M., and Bolen, D. W. (1995) Biochemistry 34, 3771-3779.
18. Hilser, V. J., and Freire, E. (1995) Anal. Biochem. 224, 465-485.
19. Baskakov, I., and Bolen, D. W. (1998) J. Biol. Chem. 273, 4831-4834.
20. Shortle, D. (1995) Advances in Prot. Chem. 46, 217-247.
21. Dill, K. A., and Shortle, D. (1991) in Annu. Rev. Biochem. (Richardson, C. C., Abelson, J. N., Meister, A., and Walsh, C. T., Eds.) pp 795-825, Annual Reviews, Palo Alto.
22. Kalman, F., Ma, S., Fox, R. O., and Horvath, C. (1995) J. Chromatogr. 705, 135-154.
23. Myers, J. K., Pace, C. N., and Scholtz, J. M. (1995) Protein Sci. 4, 2138-2148.
24. Flanagan, J. M., Kataoka, M., Fujisawa, T., and Engelman, D. M. (1993) Biochemistry 32, 10359-10370.
25. Frye, K. J., Perman, C. S., and Royer, C. A. (1996) Biochemistry 35, 10234-10239.
26. Ionescu, R. M., and Eftink, M. R. (1997) Biochemistry 36, 1129-1140.
27. Lumry, R., Biltonen, R., and Brandts, J. F. (1966) Biopoylmers 4, 917-944.
28. Bolen, D. W., and Santoro, M. M. (1988) Biochemistry 27, 8069-8074.
29. Yang, M. (1997) Ph.D. Dissertation, Denatured states of Staphylococcal nuclease and it mutants: Variable thermodynamic behavior in denaturation and its influences in denaturation free energy measurements, University of Texas Medical Branch, Galveston, TX.
30. Herrmann, L. M., and Bowler, B. E. (1997) Protein Sci. 6, 657-665.