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Dow, T.4
Foleno, B.5
Goldschmidt, R.6
Guan, J.7
Hilliard, J.J.8
Hlasta, D.J.9
Johnson, C.E.10
Johnson, S.G.11
Kanojia, R.M.12
Loeloff, M.13
Ohemeng, K.A.14
Russell, R.K.15
Sheppard, C.M.16
Fraga-Spano, S.A.17
Sui, Z.18
Weidner-Wells, M.A.19
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50 values are the mean of two experimental determinations.
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0010484293
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note
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13. In vitro minimum inhibitory concentration against a panel of Gram-positive bacteria: S. aureus (ATCC29213), MRSA (OC2089), E. faecalis (OC3041), E. faecium (OC3312, vancomycin resistant). Determination of susceptibility was performed following the broth microdilution method of the National Committee for Clinical Laboratory Standards.
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0010521961
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note
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I, the nitrogen regulatory proteins from E. coli.
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17
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0010482864
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note
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17. This is a whole cell assay to monitor the function of the VanS/VanR two-component system that activates vancomycin resistance in enterococcus. High level resistance to vancomycin is determined by a group of adjacent genes organized as an operon, whose expression is under the control of the VanS/VanR two-component system. This assay measures the enzymatic activity of firefly luciferase (luc) expressed from a protein fusion to vanH, the first gene of the operon. The presence of vancomycin causes the histidine kinase to autophosphorylate and to transfer this phosphate to VanR. Phosphorylated VanR acts as a transcriptional activator of the promoter of the operon located upstream of vanH. Thus, it upregulates transcription of downstream genes, resulting in increased luciferase expression.
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