Amount of enterotoxigenic Clostridium perfringens in meat detected by nested PCR

International Journal of Food Microbiology

Volumn 42, Issue 3, 1998, Pages 195-200

  Miwa, Norinaga ^a   Nishina, Tokuhiro ^b   Kubo, Shuichiro ^c   Atsumi, Mikio ^a   Honda, Hiroyasu ^a  

^a Shizuoka Pref Seibu Meat I   (Japan)

^b SHIZUOKA INSTITUTE OF ENVIRONMENT AND HYGIENE   (Japan)

^c NIHON UNIVERSITY   (Japan)

Author keywords

Clostridium perfringens; Enterotoxin; Meat; MPN; PCR

Indexed keywords

ENTEROTOXIN;

ARTICLE; BACTERIAL COUNT; BACTERIUM CULTURE; CLOSTRIDIUM PERFRINGENS; CONTROLLED STUDY; MEAT; NONHUMAN; POLYMERASE CHAIN REACTION;

ANIMALS; CATTLE; CHICKENS; CLOSTRIDIUM PERFRINGENS; COLONY COUNT, MICROBIAL; DNA PRIMERS; ELECTROPHORESIS, AGAR GEL; ENTEROTOXINS; MEAT; POLYMERASE CHAIN REACTION; SWINE;

EID: 0032555079     PISSN: 01681605     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1605(98)00082-8     Document Type: Article

References (16)

1. Bean N.H., Griffin P.M. Food borne disease outbreaks in the United States, 1973-1987: pathogens, vehicles, and trends. J. Food Prot. 53:1990;804-817.
2. Berndtson E., Tivemo M., Engvall A. Distribution and numbers of Campylobacter in newly slaughtered broiler chickens and hens. Int. J. Food Microbiol. 15:1992;45-50.
3. Berndtson E., Danielsson-Tham M.L., Engvall A. Campylobacter incidence on chicken farm and the spread of Campylobacter during the slaughter process. Int. J. Food Microbiol. 32:1996;35-47.
4. Franco C.M., Quinto E.J., Fente C., Rodriguez-Otero J.L., Dominguez L., Cepeda A. Determination of the principal sources of Listeria spp. contamination in poultry meat and poultry processing plant. J. Food Prot. 58:1995;1320-1325.
5. Gyobu Y. Distribution of Clostridium perfringens in the environment and the enterotoxin production by isolated strains. J. Food Hyg. Soc. Jpn. (in Japanese). 19:1978;236-241.
6. Lammerding A.M., Garcia M.M., Mann E.D., Robinson Y., Dorward W.J., Truscott R.B., Tittiger F. Prevalence of Salmonella and thermophilic Campylobacter in fresh pork, beef, veal and poultry in Canada. J. Food Prot. 51:1988;47-52.
7. Miwa N., Nishina T., Kubo S., Fujikura K. Nested polymerase chain reaction for detection of low levels of enterotoxigenic Clostridium perfringens in animal feces and meat. J. Vet. Med. Sci. 58:1996;197-203.
8. Miwa N., Nishina T., Kubo S., Atsumi M. Most probable number method combined with Nested polymerase chain reaction for detection and enumeration of enterotoxigenic Clostridium perfringens in intestinal contents of cattle, pig and chicken. J. Vet. Med. Sci. 59:1997;89-92.
9. Miwa N., Nishina T., Kubo S., Honda H. Most probable numbers of enterotoxigenic Clostridium perfringens in intestinal contents of domestic livestock detected by nested PCR. J. Vet. Med. Sci. 59:1997;557-560.
10. Oosterom J., Notermans S., Karman H., Engels G.B. Origin and prevalence of Campylobacter jejuni in poultry processing. J. Food Prot. 46:1983;339-344.
11. Saito M. Production of enterotoxin by Clostridium perfringens derived from humans, animals, foods, and the natural environment in Japan. J. Food Prot. 53:1990;115-118.
12. Saito M., Funabashi M. Alpha toxin production by enterotoxin-producing strains of Clostridium perfringens derived from patients of food poisoning outbreaks, food handlers and foods. J. Food Hyg. Soc. Jpn. (in Japanese, with English abstract). 32:1991;20-24.
13. Shandera W.X., Tacket C.O., Blake P.A. Food poisoning due to Clostridium perfringens in the United States. J. Infect. Dis. 147:1983;167-170.
14. Skjelkvale R., Uemura T. Experimental diarrhoea in human volunteers following oral administration of Clostridium perfringens enterotoxin. J. Appl. Bacteriol. 43:1977;281-286.
15. Skjelkvale R., Stringer M.F., Smart J.L. Enterotoxin production by lecithinase-positive and lecithinase-negative Clostridium perfringens isolated from food poisoning outbreaks and other sources. J. Appl. Bacteriol. 47:1979;329-339.
16. Yasukawa A., Okada Y., Kitase T., Miyamoto S. Distribution of enterotoxin-producing strains of Clostridium perfringens type A in human beings, foods and soils. J. Food Hyg. Soc. Jpn. (in Japanese, with English abstract). 16:1975;313-317.