In vitro inhibitory effects of DNA topoisomerase II by fernane-type triterpenoids isolated from a Euphorbia genus

Bioorganic and Medicinal Chemistry Letters

Volumn 8, Issue 20, 1998, Pages 2829-2832

  Wada, Shun Ichi ^a   Tanaka, Reiko ^a   Iida, Akira ^b   Matsunaga, Shunyo ^a  

^a OSAKA MEDICAL COLLEGE   (Japan)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

DNA TOPOISOMERASE (ATP HYDROLYSING); EUPHORBIA EXTRACT; TRITERPENOID;

ANTINEOPLASTIC ACTIVITY; ARTICLE; DNA REPLICATION; DRUG EFFECT; DRUG ISOLATION; ENZYME ACTIVITY; ENZYME INHIBITION; IN VITRO STUDY; KINETOPLAST; NONHUMAN;

DNA; DNA TOPOISOMERASES, TYPE II; ENZYME INHIBITORS; ETOPOSIDE; EUPHORBIACEAE; NUCLEIC ACID SYNTHESIS INHIBITORS; TRITERPENES;

EID: 0032552926     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(98)00515-0     Document Type: Article

References (12)

1. [1] Watt, P. M., Hickson I. D., Biochem. J., 1994, 303, 681-695.
2. [2] D'Arpa, P., Liu L. F., Biochim. Biophys. Acta, 1989, 989, 163-177.
3. [3] Takayasu, J., Tanaka, R., Matsunaga, S., Ueyama, H., Tokuda, H., Hasegawa, T., Nishino, A., Nishino, H., Iwashima, A., Cancer Lett., 1990, 53, 141-144.
4. [4] Tanaka, R., Matsunaga, S., Phytochemistry, 1989, 28, 3149-3154.
5. [5] Tanaka, R., Ida, T., Kita, S., Kamisako, W., Matsunaga, S., Phytochemistry , 1996, 41, 1163-1168.
6. [6] Topo II activity was measured by assessing decatenation of catenated kinetoplast DNA (0.2 μg). Reaction buffer contained 50 mM Tris-HCl, pH 8, 120 mM KCl, 10 mM MgCl2, 0.5 mM ATP, 0.5 mM dithiothreitol and 30 μg/ml BSA. Reactions were incubated with 2 units of Topo II in the presence or absence of test compounds for 30 min at 37 °C. One unit of Topo II can decatenate 0.2 μg kinetoplast DNA for 30 min at 37 °C. The reactions were stopped by adding a mixture of sarkosyl, bromophenol blue and glycerol, and electrophoresed in 1 % agarose gel with 0.5 μg/ml ethidium bromide in Tris-acetate buffer containing 1 mM EDTA. The DNA bands were visualized over UV light and photographed.
7. [7] Chow, K. C., MacDonald, T. L., Ross, W. E., Mol. Pharmacol., 1988, 34, 467-473.
8. [8] Topo II cleavage reactions contained 6 units of the enzyme and 100 ng supercoiled pBR 322 DNA in a cleavage buffer (10 mM Tris-HCl, pH 7.9, 50 mM NaCl, 50 mM KCl, 0.1 mM EDTA, 5 mM MgCl2 and 0.5 mM ATP). After incubation at 37 °C for 30 min, the reaction mixture was treated with SDS and proteinase K and electrophoresed in 1 % agarose gel.
9. [9] Drake, F. H., Hofmann, A. G., Mong, M. S., Bartus, O.J., Herzberg, R. P., Johonson, R. K., Mattern, M. R., Mirabelli, C. K., Cancer Res., 1989, 49, 2578-2583.
10. [10] Jensen, P. B., Sorensen, B. S., Demant, E. J. F., Sehested, M., Jensen, P. S., Vindelov, L., Hansen, H. H., Cancer Res., 1990, 50, 3311-3316.
11. [11] Boritzki, T. J., Wolfard, T. S., Besserer, J. A., Jackson, R. C., Fry, D. W., Biochem. Pharmacol., 1988, 37, 4063-4068.
12. [12] Tanabe, K., Ikegami, Y., Ishida, Y., Andoh, T., Cancer Res., 1991, 57, 4903-4908.