A novel carboxy-functionalized photocleavable dinucleotide analog for the selection of RNA catalysts

Tetrahedron Letters

Volumn 39, Issue 34, 1998, Pages 6157-6158

  Hausch, Felix ^a   Jäschke, Andres ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

DINUCLEOTIDE; NUCLEOTIDE DERIVATIVE; RNA; RNA LIGASE;

ARTICLE; DRUG ISOLATION; DRUG SYNTHESIS; MOLECULAR INTERACTION; RNA DEGRADATION;

EID: 0032552103     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4039(98)01265-9     Document Type: Article

References (16)

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14. 4.
15. 32P-ATP and T4 polynucleotide kinase.
16. c) 2μmol of the respective amine (4, 5, or 6), 20nmol of 1a, and 1μmol of 0.25M EDC were reacted in 4μl 2M imidazole/HCl pH=6 for 30h, and with 2μl of fresh 0.25M EDC for another 4h. Reaction mixtures were applied to a 20% denaturing gel, the products were identified by autoradiography and eluted in water. After desalting on a 4ml sephadex G10-column, lyophilized products were purified on a HPLC C-18 column (Beckman) with 30% acetonitrile in 0.1M TEAAc pH=7, Masses obtained: 1b: 4-containing fragment = 837.5 g/mol (calc. = 837.6 g/mol); 1c: 5-containing fragment = 955.7 g/mol (calc. 955.5 g/mol).