High-affinity DNA binding by the C-terminal domain of the transcriptional coactivator PC4 requires simultaneous interaction with two opposing unpaired strands and results in helix destabilization

Journal of Molecular Biology

Volumn 276, Issue 2, 1998, Pages 367-377

  Werten, Sebastiaan ^a,b   Langen, Friso W M ^a   Van Schaik, Richard ^a   Timmers, H Th Marc ^a   Meisterernst, Michael ^c   Van Der Vliet, Peter C ^a  

^a UTRECHT UNIVERSITY   (Netherlands)

^b UTRECHT UNIVERSITY   (Netherlands)

^c UNIVERSITY OF MUNICH   (Germany)

Author keywords

DNA unwinding; Heteroduplex; Protein DNA interaction; ssDNA binding; Transcriptional cofactor

Indexed keywords

DNA; DOUBLE STRANDED DNA; SINGLE STRANDED DNA; TRANSCRIPTION FACTOR;

ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; DISSOCIATION CONSTANT; DNA FOOTPRINTING; DNA HELIX; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN DOMAIN;

EID: 0032548995     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1997.1534     Document Type: Article

References (27)

1. Ballard D.W., Philbrick W.M., Bothwell A.L.M. Identification of a novel 9-kDa polypeptide from nuclear extracts. J. Biol. Chem. 263:1988;8450-8457
2. Blackwell L.J., Borowiec J.A. Human replication protein A binds single-stranded DNA in two distinct complexes. Mol. Cell. Biol. 14:1994;3993-4001
3. Bochkarev A., Pfuetzner R.A., Edwards A.M., Frappier L. Structure of the single-stranded-DNA-binding domain of replication protein A bound to DNA. Nature. 385:1997;176-181
4. Boehmer P.E., Lehman I.R. Herpes Simplex Virus type 1 ICP8 helix-destabilizing properties. J. Virol. 67:1993;711-715
5. Burley S.K., Roeder R.G. Biochemistry and structural biology of transcription factor IID (TFIID). Annu. Rev. Biochem. 65:1996;769-799
6. Carey J. Gel retardation. Methods Enzymol. 208:1991;103-117
7. Folmer R.H.A., Nilges M., Folkers P.J.M., Konings R.N.H., Hilbers C.W. A model of the complex between single-stranded DNA and the single-stranded DNA binding protein encoded by gene V of filamentous bacteriophage M13. J. Mol. Biol. 240:1994;341-357
8. Folmer R.H.A., Nilges M., Konings R.N.H., Hilbers C.W. Solution structure of the single-stranded DNA binding protein of the filamentous Pseudomonas phage Pf3 similarity to other proteins binding to single-stranded nucleic acids. EMBO J. 14:1995;4132-4142
9. Ge H., Roeder R.G. Purification, cloning, and characterization of a human coactivator, PC4 that mediates transcriptional activation of class II genes. Cell. 78:1994;513-523
10. Ge H., Zhao Y., Chait B.T., Roeder R.G. Phosphorylation negatively regulates the function of coactivator PC4. Proc. Natl Acad. Sci. USA. 91:1994;12691-12695
11. Georgaki A., Strack B., Podust V., Hübscher U. DNA unwinding activity of replication protein A. FEBS Letters. 308:1992;240-244
12. Guan Y., Zhang H., Konings R.N.H., Hilbers C.W., Terwilliger T.C., Wang A.H.-J. Crystal structures of Y41H and Y41F mutants of gene V protein from Ff phage suggest possible protein-protein interactions in the GVP-ssDNA complex. Biochemistry. 33:1994;7768-7778
13. Henry N.L., Bushnell D.A., Kornberg R.D. A yeast transcriptional stimulatory protein similar to human PC4. J. Biol. Chem. 271:1996;21842-21847
14. Holstege F.C.P., Van der Vliet P.C., Timmers H.Th.M. Opening of an RNA polymerase II promoter occurs in two distinct steps and requires the basal transcription factors IIE and IIH. EMBO J. 15:1996;1666-1677
15. Kaiser K., Meisterernst M. The human general co-factors. Trends Biol. Sci. 21:1996;342-345
16. Kaiser K., Stelzer G., Meisterernst M. The coactivator p15 (PC4) initiates transcriptional activation during TFIIA-TFIID-promoter complex formation. EMBO J. 14:1995;3520-3527
17. Knaus K., Pollock R., Guarente L. Yeast SUB1 is a suppressor of TFIIB mutations and has homology to the human co-activator PC4. EMBO J. 15:1996;1933-1940
18. Kretzschmar M., Kaiser K., Lottspeich F., Meisterernst M. A novel mediator of class II gene transcription with homology to viral immediate-early transcriptional regulators. Cell. 78:1994;525-534
19. Monaghan A., Webster A., Hay R.T. Adenovirus DNA binding protein helix destabilizing properties. Nucl. Acids Res. 22:1994;742-748
20. Pan Z.-Q., Ge H., Amin A.A., Hurwitz J. Transcription-positive cofactor 4 forms complexes with HSSB (RPA) on single-stranded DNA and influences HSSB-dependent enzymatic synthesis of Simian Virus 40 DNA. J. Biol. Chem. 271:1996;22111-22116
21. Philipova D., Mullen J.R., Maniar H.S., Lu J., Gu C., Brill S.S. A hierarchy of SSB protomers in replication protein A. Genes Dev. 10:1996;2222-2233
22. Press W.H., Flannery B.P., Teukolski S.A. Numerical Recipes in C: the Art of Scientific Computing. 1988;Cambridge University Press, New York
23. Roeder R.G. The role of general initiation factors in transcription by RNA polymerase II. Trends Biol. Sci. 21:1996;327-335
24. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd edit. :1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
25. Soengas M.S., Gutiérrez C., Salas M. Helix-destabilizing activity of Phi 29 single-stranded DNA binding protein effect on the elongation rate during strand displacement DNA replication. J. Mol. Biol. 253:1995;517-529
26. Verrijzer C.P., Tjian R. TAFs mediate transcriptional activation and promoter selectivity. Trends Biol. Sci. 21:1996;338-342
27. Zijderveld D.C., Van der Vliet P.C. Helix-destabilizing properties of the Adenovirus DNA binding protein. J. Virol. 68:1994;1158-1164