A technique for in vivo vascular perfusion fixation of the sheep central nervous system

Journal of Neuroscience Methods

Volumn 79, Issue 2, 1998, Pages 195-199

  Santoreneos, Stephen ^a   Stoodley, Marcus A ^a   Jones, Nigel R ^a   Brown, Chris J ^a  

^a UNIVERSITY OF ADELAIDE   (Australia)

Author keywords

Fixatives; Methods; Perfusion fixation; Tissue fixation; Tissue preservation

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRAIN BLOOD VESSEL; BRAIN PERFUSION; CAROTID ARTERY LIGATION; CENTRAL NERVOUS SYSTEM; NONHUMAN; PRIORITY JOURNAL; SHEEP; TISSUE FIXATION; TISSUE PRESERVATION;

ANIMALS; BRAIN; CAROTID ARTERIES; CENTRAL NERVOUS SYSTEM; CRANIOCEREBRAL TRAUMA; PERFUSION; SHEEP; SPINAL CORD; TISSUE FIXATION;

EID: 0032548981     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(97)00180-5     Document Type: Article

References (15)

1. Beach TG, Tago H, Nagai T et al. Perfusion-fixation of the human brain for immunocytochemistry: comparison with immersion-fixation. J Neurosci Methods. 19(3):1987;183-192.
2. Dae MW, Heymann MA, Jones AL A new technique for perfusion fixation and contrast enhancement of foetal lamb myocardium for electron microscopy. J Microsc. 127(3):1982;301-305.
3. Doyle CT, O'Leary JJ Editorial: The search for the universal fixative or 'magic juice'. J Pathol. 166:1992;331-332.
4. Dyce KM, Molenaar GJ The nervous system. Dyce KM, Sack WO, Wensing CJG Textbook of Veterinary Anatomy. 1995;255-327 WB Saunders Company, Philadelphia, PA.
5. Forssmann WG, Ito S, Weihe E et al. An improved perfusion fixation method for the testis. Anat Rec. 188(3):1977;307-314.
6. Hopwood D Cell and tissue fixation, 1972-1982. Histochem J. 17(4):1985;389-442.
7. Karlsson U, Schultz RL Fixation of the central nervous system for electron microscopy by aldehyde perfusion. I. Preservation with aldehyde perfusate versus direct perfusion with osmium tetroxide with special reference to membranes and extracellular space. J Ultrastruc Res. 12:1965;160-186.
8. May NDS The head and neck. Neil The Anatomy of the Sheep. 1970;133-266 University of Queensland Press, Brisbane.
9. Palay SL, McGee-Russell SM, Gordon S, Grillo MA Fixation of neural tissues for electron microscopy by perfusion fixation with solutions of osmium tetroxide. J Cell Biol. 12:1962;385-410.
10. Peters A. The fixation of central nervous system tissue and the analysis of electron micrographs of the neuropil, with special reference to the cerebral cortex. In: Nauta, Abbesson, editors. Contemporary Research Methods in Neuroanatomy. New York: Spinger,1970:56-76.
11. Roberts JC, McCrossan MV, Jones HB The case for perfusion fixation of large tissue samples for ultrastructural pathology. Ultrastruc Pathol. 14(2):1990;177-191.
12. Rostgaard J, Qvortrup K, Poulsen SS Improvements in the technique of vascular perfusion-fixation employing a fluorocarbon-containing perfusate and a peristaltic pump controlled by pressure feedback. J Microsc. 172(2):1993;137-151.
13. Smolich JJ, Canale E, Campbell GR et al. An improved perfusion technique for fixation of sheep myocardium. Anat Rec. 208(4):1984;607-611.
14. Stoodley MA, Brown SA, Brown CJ, Jones NR Arterial-pulsation dependent perivascular CSF flow into the central canal in the sheep spinal cord. J Neurosurgery. 86:686;693.
15. Williams TH, Jew JY An improved method for perfusion fixation of neural tissues for electron microscopy. Tissue Cell. 7(3):1975;407-418.