Investigation of functional aspects of the N-terminal region of elongation factor tu from Escherichia coli using a protein engineering approach

Journal of Biological Chemistry

Volumn 273, Issue 8, 1998, Pages 4387-4391

  Laurberg, Martin ^a   Mansilla, Francisco ^b   Clark, Brian F C ^b   Knudsen, Charlotte R ^b  

^a LUND UNIVERSITY   (Sweden)

^b AARHUS UNIVERSITY   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ELONGATION FACTOR;

AMINO ACID ANALYSIS; AMINO TERMINAL SEQUENCE; ARTICLE; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC ENGINEERING; NERVE CELL NETWORK; PRIORITY JOURNAL; X RAY CRYSTALLOGRAPHY;

ESCHERICHIA COLI; GTP PHOSPHOHYDROLASE-LINKED ELONGATION FACTORS; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATE; HYDROLYSIS; MUTAGENESIS, SITE-DIRECTED; PEPTIDE ELONGATION FACTOR TU; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0032548923     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.273.8.4387     Document Type: Article

References (24)

1. Kjeldgaard, M., Nyborg, J., and Clark, B. F. (1996) FASEB J. 10, 1347-1368
2. Kjeldgaard, M., and Nyborg, J. (1992) J. Mol. Biol. 223, 721-742
3. Polekhina, G., Thirup, S., Kjeldgaard, M., Nissen, P., Lippmann, C., and Nyborg, J. (1996) Structure 4, 1141-1151
4. Abel, K., Yoder, M. D., Hilgenfeld, R., and Jurnak, F. (1996) Structure 4, 1153-1159
5. Berchtold, H., Reshetnikova, L., Reiser, C. O., Schirmer, N. K., Sprinzl, M., and Hilgenfeld, R. (1993) Nature 365, 126-132
6. Kjeldgaard, M., Nissen, P., Thirup, S., and Nyborg, J. (1993) Structure 1, 35-50
7. Kawashima, T., Berthet Colominas, C., Wulff, M., Cusack, S., and Leberman, R. (1996) Nature 379, 511-518
8. Nissen, P., Kjeldgaard, M., Thirup, S., Polekhina, G., Reshetnikova, L., Clark, B. F., and Nyborg, J. (1995) Science 270, 1464-1472
9. Antonsson, B., and Leberman, R. (1984) Eur. J. Biochem. 141, 483-487
10. Sprinzl, M. (1994) Trends Biochem. Sci. 19, 245-250
11. Nissen, P., Kjeldgaard, M., Thirup, S., Clark, B. F. C., and Nyborg, J. (1996) Biochimie 78, 921-933
12. Taylor, J. W., Ott, J., and Eckstein, F. (1985) Nucleic Acids Res. 13, 8765-8785
13. Knudsen, C. R., Clark, B. F., Degn, B., and Wiborg, O. (1992) Biochem. Int. 28, 353-362
14. Sanger, F., Nicklen, S., and Coulson, A. R. (1977) Proc. Natl. Acad. Sci. U. S. A. 74, 5463-5467
15. Smith, D. B., and Johnson, K. S. (1988) Gene (Amst.) 67, 31-40
16. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Ed., pp. 13.3-13.6, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
17. Miller, D. L., and Weissbach, H. (1974) Methods Enzymol. 30, 219-232
18. Knudsen, C. R., Kjaersgard, I. V., Wiborg, O., and Clark, B. F. (1995) Eur. J. Biochem. 228, 176-183
19. Knudsen, C. R., and Clark, B. F. C. (1995) Protein Eng. 8, 1267-1273
20. von der Haar, F. (1979) Methods Enzymol. 59, 257-267
21. Andersen, C., and Wiborg, O. (1994) Eur. J. Biochem. 220, 739-744
22. Parmeggiani, A., and Sander, G. (1981) Mol. Cell. Biochem. 35, 129-158
23. Sedmark, J. J., and Grossberg, S. E. (1977) Anal. Biochem. 79, 544-552
24. Mansilla, F., Knudsen, C. R., Laurberg, M., and Clark, B. F. C. (1997) Protein Eng. 10, 927-934