Denaturant-gradient gel electrophoresis: Technical aspects and practical applications

Analytica Chimica Acta

Volumn 372, Issue 1-2, 1998, Pages 99-120

  Gianazza, Elisabetta ^a   Eberini, Ivano ^a   Santi, Ombretta ^a   Vignati, Mara ^a  

^a UNIVERSITY OF MILAN   (Italy)

Author keywords

Denaturant gradient gel electrophoresis; Electrophoresis; Protein; Protein structure; Unfolding; Urea

Indexed keywords

ANALYTIC METHOD; GEL ELECTROPHORESIS; ISOELECTRIC FOCUSING; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STRUCTURE; REVIEW; ZONE ELECTROPHORESIS;

EID: 0032547692     PISSN: 00032670     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0003-2670(98)00333-X     Document Type: Review

References (163)

1. [1] S. Hjertén, Arch. Biochem. Biophys. Suppl. I (1962) 147.
2. [2] T.E. Creighton, J. Mol. Biol. 120 (1979) 235.
3. [3] D.R. Thatcher, B. Hodson, Biochem. J. 197 (1981) 105.
4. [4] V. Rosenbaum, D. Riesner, Biophys. Chem. 26 (1987) 235.
5. [5] D. Riesner, G. Steger, T. Zimmat, R.A. Owens, M. Wagenhöfer, W. Hillen, S. Vollbach, K. Henco, Electrophoresis 10 (1989) 377.
6. [6] A. Birmes, A. Sattler, K.-H. Maurer, D. Riesner, Electrophoresis 11 (1990) 795.
7. [7] T. Arakawa, L. Hung, V. Pan, T.P. Horan, C.G. Kolvenbach, L.O. Narhi, Anal. Biochem. 208 (1993) 255.
8. [8] A. Sättler, D. Riesner, Electrophoresis 14 (1993) 782.
9. [9] C. Curtil, L. Channac, C. Ebel, P. Masson, Biochim. Biophys. Acta 1208 (1994) 1.
10. [10] D.R. Thatcher, R. Sheikh, Biochem. J. 197 (1981) 111.
11. [11] M.J. Hobart, in: J.P. Arbuthnott, J.A. Beeley (Eds.), Isoelectric Focusing, Butterworths, London, 1975, p. 275.
12. [12] R.A. Stinson, Biochem. J. 167 (1977) 65.
13. [13] K. Altland, T. Roeder, H.M. Jakin, H.-G. Zimmer, V. Neuhoff, Clin. Chem. 28 (1982) 1000.
14. [14] E. Gianazza, L. Calabresi, O. Santi, C.R. Sirtori, G. Franceschini, Biochemistry 36 (1997) 7898.
15. [15] K. Altland, S. Rauh, R. Hackler, Electrophoresis 2 (1981) 148.
16. [16] S. Kanko, K. Autio, J. Chromatogr. 324 (1985) 395.
17. [17] G.R. Johnson, A.L. Bieber, Toxicon 26 (1988) 337.
18. [18] P.J.M. van den Oetelaar, B.M. de Man, H.J. Hoenders, Biochim. Biophys. Acta 995 (1989) 82.
19. [19] P.J.M. van den Oetelaar, H.J. Hoenders, Biochim. Biophys. Acta 995 (1989) 91.
20. [20] D.E. Jenne, K. Denzel, P. Blätzinger, P. Winter, B. Obermaier, R.P. Linke, K. Altland, Proc. Natl. Acad. Sci. U.S.A. 93 (1996) 6302.
21. [21] T.O. Sippel, Anal. Biochem. 159 (1986).
22. [22] F. Kilár, S. Hjertèn, J. Chromatogr. 638 (1993) 269.
23. [23] K.R. Skelsey, M.M. Bushey, J. Chromatogr. Sci. 34 (1996) 85.
24. [24] P.B. Fernandes, R.V. Nardi, S.G. Franklin, Anal. Biochem. 91 (1978) 101.
25. [25] S. Endo, Y. Saito, A. Wada, Anal. Biochem. 131 (1983) 108.
26. [26] T.E. Creighton, J. Mol. Biol. 137 (1980) 61.
27. [27] M. Hollecker, T.E. Creighton, Biochim. Biophys. Acta 701 (1982) 395.
28. [28] D.P. Goldenberg, T.E. Creighton, Anal. Biochem. 138 (1984) 1.
29. [29] T.E. Creighton, Methods Enzymol. 131 (1986) 156.
30. [30] D.P. Goldenberg, in: T.E. Creighton (Ed.), Protein Structure: A Practical Approach, IRL Press, Oxford, 1989, p. 225.
31. [31] A. Helenius, K. Simons, Proc. Natl. Acad. Sci. U.S.A. 74 (1977) 529.
32. [32] S. Bhakdi, O.J. Bjerrum, Scand. J. Immunol. 17(suppl.)10 (1983) 313.
33. [33] G. Saglio, G. Ricco, U. Mazza, C. Camaschella, P.G. Pich, A.M. Gianni, E. Gianazza, P.G. Righetti, B. Giglioni, P. Comi, M. Gusmeroli, S. Ottolenghi, Proc. Natl. Acad. Sci. U.S.A. 76 (1979) 3420.
34. [34] K. Altland, P. Becher, A. Banzhoff, Electrophoresis 8 (1987) 293.
35. [35] G. Rovera, C. Magarian, T.W. Borun, Anal. Biochem. 85 (1977) 506.
36. [36] G. Vecchio, P.G. Righetti, M. Zanoni, G. Artoni, E. Gianazza, Anal. Biochem. 137 (1984) 410.
37. [37] A. Leroy, A. Jonas, Biochim. Biophys. Acta 1212 (1994) 285.
38. [38] P.G. Righetti, M. Chiari, E. Casale, C. Chiesa, T. Jaim, R. Shorr, J. Biochem, J. Biochem. Biophys. Methods 19 (1989) 37.
39. [39] M. Rangarajan, B. Asboth, B.S. Hartley, Biochem. J. 285 (1992) 889.
40. [40] K. Langsetmo, F.J.C. Woodward, Biochemistry 28 (1989) 3211.
41. [41] W.W. Franke, D.L. Schiller, M. Hatzfeld, S. Winter, Proc. Natl. Acad. Sci. U.S.A. 80 (1983) 7113.
42. [42] T.E. Creighton, J. Mol. Biol. 129 (1979) 411.
43. [43] M. Vilanova, F.J. Burgos, C.M. Cuchillo, F.X. Avilés, FEBS Lett. 191 (1985) 273.
44. [44] K. Cai, V. Schirch, J. Biol. Chem. 271 (1996) 2987.
45. [45] P. Carter, H. Bedouelle, G. Winter, Proc. Natl. Acad. Sci. U.S.A. 83 (1986) 1189.
46. [46] R.C. Hapak, P.J. Lammers, W.A. Palmisano, E.R. Birnbaum, M.T. Henzl, J. Biol. Chem. 264 (1989) 18751.
47. [47] D.R. Drummond, E.S. Hennessey, J.C. Sparrow, Biochem. J. 274 (1991) 301.
48. [48] J.H. Carra, P.L. Privalov, Biochemistry 34 (1995) 2034.
49. [49] R.W. Evans, J. Williams, Biochem. J. 189 (1980) 541.
50. [50] A.E. Mast, J.J. Enghild, S.V. Pizzo, G. Salvesen, Biochemistry 30 (1991) 1723.
51. [51] E. Gianazza, M. Galliano, I. Miller, Electrophoresis 18 (1997) 695.
52. [52] S. Santagati, E. Gianazza, P. Agrati, E. Vegeto, C. Patrone, G. Pollio, A. Maggi, Mol. Endocrinol. 11 (1997) 938.
53. [53] C. Gelfi, P.G. Righetti, Electrophoresis 2 (1981) 220.
54. [54] P.G. Righetti, C. Gelfi, A. Bianchi-Bosisio, Electrophoresis 2 (1981) 291.
55. [55] M.M. Santoro, D.W. Bolen, Biochemistry 27 (1988) 8063.
56. [56] J.R. Wis'niewski, K. Hessler, P. Claus, K. Zechel, Eur. J. Biochem. 243 (1997) 151.
57. [57] C.R. Matthews, M.M. Crisanti, Biochemistry 20 (1981) 784-792.
58. [58] P. Massen, J.L. Goasdoue, Biochim. Biophys. Acta 869 (1986) 304.
59. [59] S.J. Burton, A.V. Quirk, P.C. Wood, Eur. J. Biochem. 179 (1989) 379.
60. [60] R. Attanasio, G.W. Stunz, R.C. Kennedy, J. Biol. Chem. 269 (1994) 1834.
61. [61] J.J. Ewbank, T.E. Creighton, Biochemistry 32 (1993) 3694.
62. [62] M.-H. Yu, K.N. Lee, J. Kim, Nature - Structural Biology 2 (1995) 363.
63. [63] W. Kaplan, P. Hüsler, H. Klump, J. Erhardt, N. Sluis-Cremer, H. Dirr, Protein Sci. 6 (1997) 399.
64. [64] T.E. Creighton, R.H. Pain, J. Mol. Biol. 137 (1980) 431.
65. [65] C.D. Lindsay, R.H. Pain, Eur. J. Biochem. 192 (1990) 133.
66. [66] L.M. Powell, R.H. Pain, J. Mol. Biol. 224 (1992) 241.
67. [67] S.C. Tyagi, Biochem. Cell Biol. 74 (1996) 391.
68. [68] J. Williams, K. Moreton, A.D.G. Goodearl, Biochem. J. 228 (1985) 661.
69. [69] H. Yamashita, T. Nakatsuka, M. Hirose, J. Biol. Chem. 270 (1995) 29806.
70. [70] Y. Nosoh, T. Sekiguchi, Trends Biotechnol. 8 (1990) 16.
71. [71] J.E. Villafranca, E.E. Howell, S.J. Oatley, N.-H. Xuoug, Biochemistry 26 (1987) 2182.
72. [72] A.-Q. Sun, U. Yüksel, R.W. Gracy, Arch. Biochem. Biophys. 293 (1992) 382.
73. [73] J. Vendrell, F.X. Avilés, M. Vilanova, C.H. Turner, C. Crane-Robinson, Biochem. J. 267 (1990) 213.
74. [74] J. Wilson, R.F. Kelley, W. Shalongo, D. Lowery, E. Stellwagen, Biochemistry 25 (1986) 7560.
75. [75] N.B. Calcaterra, G.A. Picó, E.G. Orellano, J. Ottado, N. Carrillo, E.A. Ceccarelli, Biochemistry 34 (1995) 12842.
76. [76] J.D. Klemm, J.A. Wozniak, T. Alber, D.P. Goldenberg, Biochemistry 30 (1991) 589.
77. [77] R.M. France, S.H. Grossman, Arch. Biochem. Biophys. 326 (1996) 93.
78. [78] H. Keyvani-Amineh, M. Diouri, J.G. Guillemette, J.M. Weber, J. Biol. Chem. 270 (1995) 23250.
79. [79] P. Wingfield, R. Benedict, G. Turcatti, B. Allet, J.-J. Mermod, J. DeLamarter, M.G. Simona, K. Rose, Biochem. J. 256 (1988) 213.
80. [80] K.S. Siddiqui, M. Rangarajan, B.S. Hartley, A. Kitmitto, M. Panico, I.P Blench, H.R. Morris, Biochem. J. 289 (1993) 201.
81. [81] A.A. Paladini, G. Weber, L. Erijman, Anal. Biochem. 218 (1994) 364.
82. [82] I. Inoue, M. Rechsteiner, J. Biol. Chem. 269 (1994) 29241.
83. [83] R. Dengler, F. Lottspeich, W. Oberthur, A.E. Mast, B. Emmerich, Biol. Chem. Hoppe-Seyler 376 (1995) 165.
84. [84] F. Gentile, B.M. Veneziani, C. Sellitto, Anal. Biochem. 244 (1997) 228.
85. [85] K. Nishigaki, M. Tsubota, T. Miura, Y. Chonan, Y. Husimi, J. Biochem. 111 (1992) 144.
86. [86] K. Nishigaki, T. Miura, M. Tsubota, A. Sutoh, N. Amano, Y. Husimi, J. Biochem. 111 (1992) 151.
87. [87] T.E. Creighton, D. Shortle, 242 (1994) 670.
88. [88] A.V. Oleinikov, B. Perroud, B. Wang, R.R. Traut, J. Biol. Chem. 268 (1993) 917.
89. [89] D.A. Lomas, P.R. Elliott, W.-S.W. Chang, M.R. Wardell, R.W. Carrell, J. Biol. Chem. 270 (1995) 5282.
90. [90] A.F. Strachan, E.G. Shephard, D.U. Bellstedt, G.A. Coetzee, D.R. van der Westhuyzen, F.C. de Beer, Biochem. J. 263 (1989) 365.
91. [91] T.E. Creighton (Ed.), Protein Folding, Freeman, New York, 1992.
92. [92] I. Pastan, B.K. Lee, C.-I. Tsou, Protein folding: thematic issue, FASEB J. 10 (1996).
93. [93] A.N. Fedorov, B. Friguet, L. Djavadi-Ohaniance, Y.B. Alakhov, M.E. Goldberg, J. Mol. Biol. 228 (1992) 351.
94. [94] A.N. Fedorov, D.A. Dolgikh, V.V. Chemeris, B.K. Chernov, A.V. Finkelstein, A.A. Schluga, Y.B. Alakhov, M.P. Kirpichnikov, O.B. Ptitsyn, J. Mol. Biol. 225 (1992) 927.
95. [95] M.M. Altamirano, J.A. Plumbridge, H.A. Barba, M.L. Calcagno, Biochem. J. 295 (1993) 645.
96. [96] T. Wakagi, C.H. Lee, T. Oshima, Biochim. Biophys. Acta 1120 (1992) 289-296.
97. [97] K.A. Ferguson, Metabolism 13 (1964) 985.
98. [98] D.A. Lomas, P.R. Elliott, S.K. Sidhar, R.C. Foreman, J.T. Finch, D.W. Cox, J.C. Whisstock, R.W. Carrell, J. Biol. Chem. 270 (1995) 16864.
99. [99] C.N. Pace, Methods Enzymol. 131 (1986) 266.
100. [100] H. Utiyama, R.L. Baldwin, Methods Enzymol. 131 (1986) 51.
101. [101] F.X. Schmid, Methods Enzymol. 131 (1986) 70.
102. [102] J. King, M.-H. Yu, Methods Enzymol. 131 (1986) 250.
103. [103] A.L. Fink, Methods Enzymol. 131 (1986) 173.
104. [104] H. Taniuchi, G.R. Parr, M.A. Juillerat, Methods Enzymol. 131 (1986) 185.
105. [105] R. Jaenicke, R. Rudolph, Methods Enzymol. 131 (1986) 218.
106. [106] Y. Nozaki, C. Tanford, Methods Enzymol. 11 (1967) 715.
107. [107] G.E. Perlmann, Methods Enzymol. 25 (1972) 413.
108. [108] G. Di Sabato, M. Ottesen, Methods Enzymol. 11 (1967) 734.
109. [109] S.W. Englander, J.J. Englander, Methods Enzymol. 25 (1972) 406.
110. [110] S.W. Englander, J.J. Englander, Methods Enzymol. 49 (1978) 24.
111. [111] P.S. Kim, Methods Enzymol. 131 (1986) 136.
112. [112] J.A. Rupley, Methods Enzymol. 11 (1967) 905.
113. [113] J.F. Brandts, L.-N. Lin, Methods Enzymol. 131 (1986) 107.
114. [114] R.K. Brown, Methods Enzymol. 11 (1967) 917.
115. [115] T.E. Creighton, Methods Enzymol. 131 (1986) 83.
116. [116] H.R. Horton, D.E.J. Koshland, Methods Enzymol. 11 (1967) 857.
117. [117] M.E. Kirtley, D.E.J. Koshland, Methods Enzymol. 11 (1967) 866.
118. [118] M.E. Kirtley, D.E.J. Koshland, Methods Enzymol. 25 (1972) 578.
119. [119] T.T. Herskovits, Methods Enzymol. 11 (1967) 748.
120. [120] J.W. Donovan, Methods Enzymol. 27 (1973) 525.
121. [121] T. Ichikawa, H. Terada, Biochim. Biophys. Acta 671 (1981) 33.
122. [122] R. Ragone, G. Colonna, C. Balestriero, L. Servillo, G. Trace, Biochemistry 23 (1984) 1871.
123. [123] J.W. Donovan, Methods Enzymol. 27 (1973) 525.
124. [124] L. Brand, B. Witholt, Methods Enzymol. 11 (1967) 776.
125. [125] J. Yguerabide, Methods Enzymol. 25 (1972) 498.
126. [126] G.D. Fasman, B. Bablouzian, Methods Enzymol. 27 (1973) 811.
127. [127] M.G. Badea, L. Brand, Methods Enzymol. 61 (1979) 378.
128. [128] S.S. Lehrer, P.C. Leavis, Methods Enzymol. 49 (1978) 222.
129. [129] M. Sokolovsky, E. Daniel, Methods Enzymol. 49 (1978) 236.
130. [130] A.J. Adler, N.J. Greenfield, G.D. Fasman, Methods Enzymol. 27 (1973) 675.
131. [131] J.T. Yang, K.H. Chau, Methods Enzymol. 27 (1973) 736.
132. [132] B. Holmquist, B.L. Vallee, Methods Enzymol. 49 (1978) 149.
133. [133] D.H. Turner, Methods Enzymol. 49 (1978) 199.
134. [134] E.S. Stevens, Methods Enzymol. 49 (1978) 214.
135. [135] P.C. Kahn, Methods Enzymol. 61 (1979) 339.
136. [136] J.T. Yang, C.-S.C. Wu, H.M. Martinez, Methods Enzymol. 130 (1986) 208.
137. [137] B. Holmquist, Methods Enzymol. 130 (1986) 270.
138. [138] A.M. Labhardt, Methods Enzymol. 131 (1986) 126.
139. [139] H. Susi, Methods Enzymol. 25 (1972) 381.
140. [140] S.N. Timasheff, H. Susi, J.A. Rupley, Methods Enzymol. 27 (1973) 548.
141. [141] H. Susi, D.M. Byler, Methods Enzymol. 130 (1986) 290.
142. [142] M.C. Tobin, Methods Enzymol. 25 (1972) 473.
143. [143] H.E. Van Wart, H.A. Scheraga, Methods Enzymol. 49 (1978) 67.
144. [144] R.W. Williams, Methods Enzymol. 130 (1986) 311.
145. [145] B. Hudson, L. Mayne, Methods Enzymol. 130 (1986) 331.
146. [146] J.L. Markley, Methods Enzymol. 25 (1972) 605.
147. [147] D.H. Meadows, Methods Enzymol. 25 (1972) 638.
148. [148] W.D. Phillips, Methods Enzymol. 27 (1973) 825.
149. [149] J.E. McKie, J.F. Brandts, Methods Enzymol. 25 (1972) 257.
150. [150] M. Kasai, F. Oosawa, Methods Enzymol. 25 (1972) 289.
151. [151] E. Uhlenhopp, B.H. Zimm, Methods Enzymol. 27 (1973) 483.
152. [152] D.S. Auld, Methods Enzymol. 61 (1979) 318.
153. [153] M.A. Marini, C.J. Martin, Methods Enzymol. 27 (1973) 590.
154. [154] K.S. Krishnan, J.F. Brandts, Methods Enzymol. 49 (1978) 3.
155. [155] N. Langerman, R.L. Biltonen, Methods Enzymol. 61 (1979) 261.
156. [156] R.L. Biltonen, R.L. Langerman, Methods Enzymol. 61 (1979) 287.
157. [157] P.L. Privalov, S.A. Potekhin, Methods Enzymol. 131 (1986) 4.
158. [158] R.M. Oliver, Methods Enzymol. 27 (1973) 616.
159. [159] H.P. Erickson, W.A. Voter, K. Leonard, Methods Enzymol. 49 (1978) 39.
160. [160] Y. Nozaki, Methods Enzymol. 27 (1973) 491.
161. [161] L.C. Craig, Methods Enzymol. 11 (1967) 870.
162. [162] V.N. Uversky, O.B. Ptisyn, Biochemistry 32 (1994) 2782.
163. [163] M. Dadlez, Biochemistry 36 (1997) 2788.