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Volumn 120, Issue 32, 1998, Pages 8020-8025

Characterization of polyethylene glycolated horseradish peroxidase in organic solvents: Generation and stabilization of transient catalytic intermediates at low temperature

Author keywords

[No Author keywords available]

Indexed keywords

BENZENE; CHLOROBENZENE; GUAIACOL; HORSERADISH PEROXIDASE; MACROGOL; ORGANIC SOLVENT;

EID: 0032547243     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja9714696     Document Type: Article
Times cited : (37)

References (41)
  • 2
    • 0002154654 scopus 로고
    • Everse, J., Everse, K. E., Grisham, M. B., Eds.; CRC Press: Boca Raton, FL
    • (b) Dunford, H. B. In Peroxidases in Chemistry and Biology; Everse, J., Everse, K. E., Grisham, M. B., Eds.; CRC Press: Boca Raton, FL, 1991; Vol. 2, pp 1-24.
    • (1991) Peroxidases in Chemistry and Biology , vol.2 , pp. 1-24
    • Dunford, H.B.1
  • 7
    • 0001001527 scopus 로고
    • (b) George, P. Nature 1952, 169, 612-613.
    • (1952) Nature , vol.169 , pp. 612-613
    • George, P.1
  • 30
    • 3543018038 scopus 로고    scopus 로고
    • note
    • -1 (in ref 1a), respectively, the intensity of Soret of PEG-HRP compound I in benzene (Figure 4) is greater than we expected. Since the extinction coefficient of compound I for PEG-HRP in buffer is approximately half of that of ferric PEG-HRP (Figure 3), the nature of compound I in organic media could be different.
  • 31
    • 3543010884 scopus 로고    scopus 로고
    • note
    • obs2) to accumulate compound I. The results are consistent with the hypothesis that bulky substrates cannot readily reach the heme of PEG-HRP in organic solvents. However, the spectrum generated with the alkyl hydroperoxide is the same as that with hydrogen peroxide.
  • 32
    • 3543024000 scopus 로고    scopus 로고
    • note
    • 2 in benzene at 7.5°C was also found to be nonlinear, and the rate of compound I formation reached a plateau when more than 10 equiv of hydrogen peroxide were used.
  • 36
    • 3543027553 scopus 로고    scopus 로고
    • note
    • The amount of water in PEG-HRP benzene solution was 109 ± 3 ppm, when 1 μM of PEG-HRP in dry benzene (water content 4.4 ± 0.3 ppm) was prepared.
  • 37
    • 3543031144 scopus 로고    scopus 로고
    • note
    • Soret (ferric) = 0.82, 0.71. and 0.66 in the presence of 142, 216, and 305 ppm of water in chlorobenzene, respectively).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.