A calcium sensor homolog required for plant salt tolerance

Science

Volumn 280, Issue 5371, 1998, Pages 1943-1945

  Liu, Jiping ^a   Zhu, Jian Kang ^a  

^a UNIVERSITY OF ARIZONA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALCIUM; CROPS; SODIUM; SOILS;

ARABIDOPSIS THALIANA; CALCIUM SENSOR; PLANT GROWTH; PLANT SALT TOLERANCE; SALINIZED SALT;

PLANTS (BOTANY);

AGRICULTURE; ARABIDOPSIS; ARTICLE; CALCIUM CELL LEVEL; NONHUMAN; PLANT GROWTH; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; SIGNAL TRANSDUCTION; SOIL;

AMINO ACID SEQUENCE; ANIMALS; ARABIDOPSIS; ARABIDOPSIS PROTEINS; BINDING SITES; CALCINEURIN; CALCIUM; CALCIUM-BINDING PROTEINS; CHROMOSOME MAPPING; CLONING, MOLECULAR; GENES, PLANT; ION TRANSPORT; MOLECULAR SEQUENCE DATA; MUTATION; OPEN READING FRAMES; PLANT PROTEINS; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION; SODIUM;

ANIMALIA; ARABIDOPSIS; ARABIDOPSIS THALIANA;

EID: 0032546821     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.280.5371.1943     Document Type: Article

References (39)

1. E. Epstein et al., Science 210, 399 (1980).
2. H. Greenway and R. Munns, Annu. Rev. Plant Physiol. 31, 149 (1980); X. Niu, R. A. Bressan, P. M. Hasegawa, J. M. Pardo, Plant Physiol. 109, 735 (1995).
3. H. Greenway and R. Munns, Annu. Rev. Plant Physiol. 31, 149 (1980); X. Niu, R. A. Bressan, P. M. Hasegawa, J. M. Pardo, Plant Physiol. 109, 735 (1995).
4. E. Epstein, Mineral Nutrition of Plants: Principles and Perspectives (Wiley, New York, 1972).
5. J. R. Murguia, J. M. Belles, R. Serrano, Science 267, 232 (1995).
6. S.-J. Wu, L. Ding, J.-K. Zhu, Plant Cell 8, 617 (1996).
7. P. A. LaHaye and E. Epstein, Science 166, 395 (1969).
8. J. Lynch, V. S. Polito, A. Läuchli, Plant Physiol. 90, 1271 (1989).
9. A. Läuchli, in Calcium in Plant Growth and Development, vol. 4 of American Society of Plant Physiologists Symposium Series, R. T. Leonard and P. K. Hepler, Eds. (American Society of Plant Physiologists, Rockville, MD, 1990), pp. 26-35.
10. J. Liu and J.-K. Zhu, Proc. Natl. Acad. Sci. U.S.A. 94, 14960 (1997).
11. Overlapping YAC clones were isolated from YAC libraries of Arabidopsis [E. R. Ward and G. C. Jen, Plant Mol. Biol. 14, 561 (1990); J. R. Ecker, Methods 1, 186 (1990); E. Grill and C. Somerville, Mol. Gen. Genet. 226, 484 (1991)]. Overlapping BAC clones were isolated from BAG libraries of Arabidopsis [S. Choi, R. A. Creelman, J. E. Mullet, R. A. Wing, Weeds World 2, 17 (1995)]. End probes were isolated from the YAC and BAC clones by inverse PCR. Restriction fragment length polymorphism (RFLP) analysis was performed with F2 sos3 plants with recombination break points in either the nga139-SOS3 region (four recombinants) or the SOS3-CDPK9 region (14 recombinants).
12. Overlapping YAC clones were isolated from YAC libraries of Arabidopsis [E. R. Ward and G. C. Jen, Plant Mol. Biol. 14, 561 (1990); J. R. Ecker, Methods 1, 186 (1990); E. Grill and C. Somerville, Mol. Gen. Genet. 226, 484 (1991)]. Overlapping BAC clones were isolated from BAG libraries of Arabidopsis [S. Choi, R. A. Creelman, J. E. Mullet, R. A. Wing, Weeds World 2, 17 (1995)]. End probes were isolated from the YAC and BAC clones by inverse PCR. Restriction fragment length polymorphism (RFLP) analysis was performed with F2 sos3 plants with recombination break points in either the nga139-SOS3 region (four recombinants) or the SOS3-CDPK9 region (14 recombinants).
13. Overlapping YAC clones were isolated from YAC libraries of Arabidopsis [E. R. Ward and G. C. Jen, Plant Mol. Biol. 14, 561 (1990); J. R. Ecker, Methods 1, 186 (1990); E. Grill and C. Somerville, Mol. Gen. Genet. 226, 484 (1991)]. Overlapping BAC clones were isolated from BAG libraries of Arabidopsis [S. Choi, R. A. Creelman, J. E. Mullet, R. A. Wing, Weeds World 2, 17 (1995)]. End probes were isolated from the YAC and BAC clones by inverse PCR. Restriction fragment length polymorphism (RFLP) analysis was performed with F2 sos3 plants with recombination break points in either the nga139-SOS3 region (four recombinants) or the SOS3-CDPK9 region (14 recombinants).
14. Overlapping YAC clones were isolated from YAC libraries of Arabidopsis [E. R. Ward and G. C. Jen, Plant Mol. Biol. 14, 561 (1990); J. R. Ecker, Methods 1, 186 (1990); E. Grill and C. Somerville, Mol. Gen. Genet. 226, 484 (1991)]. Overlapping BAC clones were isolated from BAG libraries of Arabidopsis [S. Choi, R. A. Creelman, J. E. Mullet, R. A. Wing, Weeds World 2, 17 (1995)]. End probes were isolated from the YAC and BAC clones by inverse PCR. Restriction fragment length polymorphism (RFLP) analysis was performed with F2 sos3 plants with recombination break points in either the nga139-SOS3 region (four recombinants) or the SOS3-CDPK9 region (14 recombinants).
15. Cosmid clones were isolated from an Arabidopsis genomic library [N. Olszewski, F. Martin, F. Ausubel, Nucleic Acids Res. 16, 10765 (1988)] by hybridization with the 1E7L BAC end probe. Cosmid DNAs were transformed into sos3-1 plants by a modified vacuum infiltration method [N. Bechtold, J. Ellis, G. Pelletier, C. R. Acad. Sci. Paris 316, 1194 (1993)].
16. Cosmid clones were isolated from an Arabidopsis genomic library [N. Olszewski, F. Martin, F. Ausubel, Nucleic Acids Res. 16, 10765 (1988)] by hybridization with the 1E7L BAC end probe. Cosmid DNAs were transformed into sos3-1 plants by a modified vacuum infiltration method [N. Bechtold, J. Ellis, G. Pelletier, C. R. Acad. Sci. Paris 316, 1194 (1993)].
17. GenBank accession number AB006701.
18. A genomic DNA fragment spanning from 1100 bp upstream of the initiation codon to 1587 bp downstream of the initiation codon was cloned into the pBIN19 vector between the Hind III and Xba I sites. The construct was transformed into sos3 plants by a modified vacuum infiltration method. Primary transformants and their progeny were tested for NaCl tolerance with the root-bending assay (5).
19. A labeled Bam HI-Kpn I fragment of SOS3 genomic DNA (Fig. 1B) was used as probe to screen an Arabidopsis cDNA library [J. J. Kieber, M. Rothenburg, G. Roman, K. A. Feldmann, J. R. Ecker, Cell 72, 427 (1993)]. RT-PCR was performed with mRNA from Aabidopsis leaves.
20. N. D. Moncrief, R. H. Kretsinger, M. Goodman, J. Mol. Evol. 30, 522 (1990).
21. M. S. Cyert and J. Thorner, Mol. Cell. Biol. 12, 3460 (1992).
22. N. C. Schaad et al., Proc. Natl. Acad. Sci. U.S.A. 93, 9253 (1996).
23. A. M. Dizhoor et al., Science 251, 915 (1991); D. H.-F. Teng, C.-K. Chen, J. B. Hurley, J. Biol. Chem. 269, 31900 (1994).
24. A. M. Dizhoor et al., Science 251, 915 (1991); D. H.-F. Teng, C.-K. Chen, J. B. Hurley, J. Biol. Chem. 269, 31900 (1994).
25. P. Olafsson, T. Wang, B. Lu, Proc. Natl. Acad. Sci. U.S.A. 92, 8001 (1995).
26. D. A. Towler, J. I. Gordon, S. P. Adams, L. Glaser, Annu. Rev. Biochem. 57, 69 (1988).
27. Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; X, any amino acid; and Y, Tyr.
28. S. Luan, W. Li, F. Rusnak, S. M. Assmann, S. L. Schreiber, Proc. Natl. Acad. Sci. U.S.A. 90, 2202 (1993); G. J. Allen and D. Sanders, Plant Cell 7, 1473 (1995).
29. S. Luan, W. Li, F. Rusnak, S. M. Assmann, S. L. Schreiber, Proc. Natl. Acad. Sci. U.S.A. 90, 2202 (1993); G. J. Allen and D. Sanders, Plant Cell 7, 1473 (1995).
30. N. A. Clipstone and G. R. Crabtree, Nature 357, 695 (1992); G. Tong, D. Shepherd, C. E. Jahr, Science 267, 1510 (1995); M. A. Lawson and F. R. Maxfield, Nature 377, 75 (1995); A. Aperia, F. Ibarra, L.-B. Svensson, C. Klee, P. Greengard, Proc. Natl. Acad. Sci. U.S.A. 89, 7394 (1992).
31. N. A. Clipstone and G. R. Crabtree, Nature 357, 695 (1992); G. Tong, D. Shepherd, C. E. Jahr, Science 267, 1510 (1995); M. A. Lawson and F. R. Maxfield, Nature 377, 75 (1995); A. Aperia, F. Ibarra, L.-B. Svensson, C. Klee, P. Greengard, Proc. Natl. Acad. Sci. U.S.A. 89, 7394 (1992).
32. N. A. Clipstone and G. R. Crabtree, Nature 357, 695 (1992); G. Tong, D. Shepherd, C. E. Jahr, Science 267, 1510 (1995); M. A. Lawson and F. R. Maxfield, Nature 377, 75 (1995); A. Aperia, F. Ibarra, L.-B. Svensson, C. Klee, P. Greengard, Proc. Natl. Acad. Sci. U.S.A. 89, 7394 (1992).
33. N. A. Clipstone and G. R. Crabtree, Nature 357, 695 (1992); G. Tong, D. Shepherd, C. E. Jahr, Science 267, 1510 (1995); M. A. Lawson and F. R. Maxfield, Nature 377, 75 (1995); A. Aperia, F. Ibarra, L.-B. Svensson, C. Klee, P. Greengard, Proc. Natl. Acad. Sci. U.S.A. 89, 7394 (1992).
34. T. Nakamura et al., EMBO J. 12, 4063 (1993); I. Mendoza, F. Rubio, A. Rodriguez-Navarro, J. M. Pardo, J. Biol. Chem. 269, 8792 (1994).
35. T. Nakamura et al., EMBO J. 12, 4063 (1993); I. Mendoza, F. Rubio, A. Rodriguez-Navarro, J. M. Pardo, J. Biol. Chem. 269, 8792 (1994).
36. S. Kawamura, O. Hisatomi, S. Kayada, F. Tokunaga, C.-H. Kuo, J. Biol. Chem. 268, 14579 (1993).
37. H. Knight, A. J. Trewavas, M. R. Knight, Plant J. 12, 1067 (1997).
38. J. Liu and J.-K. Zhu, data not shown.
39. We sincerely thank R. T. Leonard, H. Bohnert, F. Tax, Z. Liu, and P. M. Hasegawa for helpful discussions; M. Ishitani, B. Stevenson, and J. Ma for technical assistance; and X. Lin for DNA sequence analysis. Supported by U.S. Department of Agriculture.