Identification of the Rhizobium meliloti alcohol dehydrogenase gene (adhA) and heterologous expression in Alcaligenes eutrophus

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1384, Issue 2, 1998, Pages 197-203

  Willis, Laura B ^a   Walker, Graham C ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

Alcaligenes eutrophus; Alcohol dehydrogenase; Poly beta hydroxybutyrate; Rhizobium meliloti

Indexed keywords

ALCOHOL DEHYDROGENASE;

ARTICLE; ENZYME ACTIVITY; GENE EXPRESSION; GENE ISOLATION; GENETIC ANALYSIS; GENETIC ORGANIZATION; NONHUMAN; PRIORITY JOURNAL; SINORHIZOBIUM MELILOTI; WAUTERSIA EUTROPHA;

ALCALIGENES; ALCOHOL DEHYDROGENASE; AMINO ACID SEQUENCE; BASE SEQUENCE; MOLECULAR SEQUENCE DATA; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SINORHIZOBIUM MELILOTI;

EID: 0032546625     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(98)00048-X     Document Type: Article

References (17)

1. Friedman A.M., Long S.R., Brown S.E., Biukema W.J., Ausubel F.M. Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants. Gene. 18:1982;289-296.
2. Peoples O.P., Sinskey A.J. Poly-β-hydroxybutyrate (PHB) biosynthesis in Alcaligenes eutrophus H16: identification and characterization of the PHB polymerase gene (phbC). J. Biol. Chem. 264:1989;15298-15303.
3. Finan T.M., Kunkel B., de Vos G.F., Signer E.R. Second symbiotic megaplasmid in Rhizobium meliloti carrying exopolysaccharide and thiamine synthesis genes. J. Bacteriol. 167:1986;66-72.
4. Philipp W.J., Poulet S., Eiglmeier K., Pascopella L., Balasubramanian V., Heym B., Bergh S., Bloom B.R., Jacobs W.R.J., Cole S.T. An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae. Proc. Natl. Acad. Sci. U.S.A. 93:1996;3132-3137.
5. Sakoda H., Imanaka T. Cloning and sequencing of the gene coding for alcohol dehydrogenase of Bacillus stearothermophilus and rational shift of the optimum pH. J. Bacteriol. 174:1992;1397-1402.
6. Cannio R., Rossi M., Bartolucci S. A few amino acid substitutions are responsible for the higher thermostability of a novel NAD(+)-dependent bacillar alcohol dehydrogenase. Eur. J. Biochem. 222:1994;345-352.
7. Robinson G.A., Bailey C.J., Dowds B.C. Gene structure and amino acid sequences of alcohol dehydrogenases of Bacillus stearothermophilus. Biochim. Biophys. Acta. 1218:1994;432-434.
8. Ecklund H., Nordström B., Zeppezauer E., Söderlund G., Ohlsson I., Boiwe T., Söderberg B.-O., Tapia O., Brändén C.-I. Three-dimensional structure of horse liver alcohol dehydrogenase at 2.4 Å resolution. J. Mol. Biol. 102:1976;27-59.
9. Dowds B.C., Sheehan M.C., Bailey C.J., McConnell D.J. Cloning and characterization of the gene for a methanol-utilizing alcohol dehydrogenase from Bacillus stearothermophilus. Gene. 68:1988;11-22.
10. G.P. Manchenko, Handbook of Detection of Enzymes on Electrophoretic Gels, CRC Press, Boca Raton, FL, 1994.
11. Reid M.F., Fewson C.A. Molecular characterization of microbial alcohol dehydrogenases. Crit. Rev. Microbiol. 20:1994;13-56.
12. Rigaud J., Trinchant J.-C. Isolement d'une alcool déshydrogénase chez Rhizobium et rôle dans le métabolisme indolique. Physiol. Plant. 28:1973;160-165.
13. Irigoyen J.J., Emerich D.W., Sánchez-Diáz M. Phosphoenolpyruvate carboxylase, malate and alcohol dehydrogenase activities in alfalfa (Medicago sativa) nodules under water stress. Physiol. Plant. 84:1992;61-66.
14. Sobral B.W.S., Honeycutt R.J., Atherly A.G., McClelland M. Electrophoretic separation of the three Rhizobium meliloti replicons. J. Bacteriol. 173:1991;5173-5180.
15. Steinbüchel A., Schlegel H.G. Excretion of pyruvate by mutants of Alcaligenes eutrophus, which are impaired in the accumulation of poly(β-hydroxybutyric acid) (PHB), under conditions permitting synthesis of PHB. Appl. Microbiol. Biotechnol. 31:1989;168-175.
16. Jendrossek D., Steinbüchel A., Schlegel H.G. Alcohol dehydrogenase gene from Alcaligenes eutrophus: subcloning, heterologous expression in Escherichia coli, sequencing and location of Tn5 insertions. J. Bacteriol. 170:1988;5248-5256.
17. L.B. Willis, G.C. Walker, A novel Rhizobium meliloti operon encodes an α-glucosidase and a periplasmic binding protein dependent system for the transport of α-glucosides, submitted to J. Bacteriol.