Microscale nutrient patches in planktonic habitats shown by chemotactic bacteria

Science

Volumn 282, Issue 5397, 1998, Pages 2254-2256

  Blackburn, Nicholas ^a   Fenchel, Tom ^a   Mitchell, Jim ^b  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

^b FLINDERS UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

SEA WATER;

ARTICLE; BACTERIAL FLORA; BACTERIUM; CYTOLYSIS; ECOLOGY; EXCRETION; NONHUMAN; NUTRIENT; PLANKTON; PRIORITY JOURNAL; PROTOZOON; SYMBIOSIS;

BACTERIA (MICROORGANISMS); PROTOZOA;

EID: 0032545478     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.282.5397.2254     Document Type: Article

References (34)

1. J. C. Goldman, Bull. Mar. Sci. 35, 462 (1984).
2. E. M. Purcell, Am. J. Phys. 45, 3 (1977).
3. G. A. Jackson, Limnol. Oceanogr. 32, 1253 (1987).
4. W. Bell and R. Mitchell, Biol. Bull. 143, 265 (1972).
5. J. G. Mitchell, A. Okubo, J. A. Fuhrman, Nature 316, 58 (1985); J. D. Bowen, K. D. Stolzenbach, S. W. Chisholm, Limnol. Oceanogr. 38, 36 (1993).
6. J. G. Mitchell, A. Okubo, J. A. Fuhrman, Nature 316, 58 (1985); J. D. Bowen, K. D. Stolzenbach, S. W. Chisholm, Limnol. Oceanogr. 38, 36 (1993).
7. N. Blackburn, unpublished observations.
8. _, F. Azam, Å. Hagström, Limnol. Oceanogr. 42, 613 (1997).
9. Samples were taken, immediately before observation, from the vicinity of algal mats in a large seawater aquarium with high through-flow. The microbial community could be characterized as being rich and diverse. Identical communities have been observed in completely natural habitats. A standard microscope fitted with a dark-field condenser was used for observation with a 10× objective giving a depth of field of ∼20 μm. The magnification was increased for some recordings with a magnifying lens. Samples were observed in a chamber made by a 1.5-mm-thick rubber O-ring placed on top of a microscope slide and covered with a cover slip. A fiber optic light source was used for illumination to minimize heat transfer. A standard video camera and VCR were used for recordings.
10. A. Andersson, C. Lee, F. Azam, Å. Hagström, Mar. Ecol. Prog. Ser. 23, 99 (1985); P. A. Jumars, D. L. Penry, J. A. Baross, M. J. Perry, B. W. Frost, Deep-Sea Res. 36, 483 (1989).
11. A. Andersson, C. Lee, F. Azam, Å. Hagström, Mar. Ecol. Prog. Ser. 23, 99 (1985); P. A. Jumars, D. L. Penry, J. A. Baross, M. J. Perry, B. W. Frost, Deep-Sea Res. 36, 483 (1989).
12. J. T. Lehman and D. Scavia, Proc. Natl. Acad. Sci. U.S.A. 79, 5001 (1982).
13. P. J. L. Williams and L. R. Muir, in Ecohydrodynamics, J. C. J. Nihoul, Ed. (Elsevier, New York, 1981), pp. 209-218; D. J. Currie, J. Plankton Res. 6, 591 (1984); G. A. Jackson, Nature 284, 439 (1980).
14. P. J. L. Williams and L. R. Muir, in Ecohydrodynamics, J. C. J. Nihoul, Ed. (Elsevier, New York, 1981), pp. 209-218; D. J. Currie, J. Plankton Res. 6, 591 (1984); G. A. Jackson, Nature 284, 439 (1980).
15. P. J. L. Williams and L. R. Muir, in Ecohydrodynamics, J. C. J. Nihoul, Ed. (Elsevier, New York, 1981), pp. 209-218; D. J. Currie, J. Plankton Res. 6, 591 (1984); G. A. Jackson, Nature 284, 439 (1980).
16. D. A. Brown and H. C. Berg, Proc. Natl. Acad. Sci. U.S.A. 71, 1388 (1974).
17. T. Fenchel, Microbiology 140, 3109 (1994); J. G. Mitchell et al., Appl. Environ. Microbiol. 61, 877 (1995); J. G. Mitchell, L. Pearson, S. Dillon, K. Kantalis, ibid., p. 4436; J. G. Mitchell, L. Pearson, S. Dillon, ibid. 62, 3716 (1996); G. M. Barbara and J. G. Mitchell, ibid., p. 3985.
18. T. Fenchel, Microbiology 140, 3109 (1994); J. G. Mitchell et al., Appl. Environ. Microbiol. 61, 877 (1995); J. G. Mitchell, L. Pearson, S. Dillon, K. Kantalis, ibid., p. 4436; J. G. Mitchell, L. Pearson, S. Dillon, ibid. 62, 3716 (1996); G. M. Barbara and J. G. Mitchell, ibid., p. 3985.
19. T. Fenchel, Microbiology 140, 3109 (1994); J. G. Mitchell et al., Appl. Environ. Microbiol. 61, 877 (1995); J. G. Mitchell, L. Pearson, S. Dillon, K. Kantalis, ibid., p. 4436; J. G. Mitchell, L. Pearson, S. Dillon, ibid. 62, 3716 (1996); G. M. Barbara and J. G. Mitchell, ibid., p. 3985.
20. T. Fenchel, Microbiology 140, 3109 (1994); J. G. Mitchell et al., Appl. Environ. Microbiol. 61, 877 (1995); J. G. Mitchell, L. Pearson, S. Dillon, K. Kantalis, ibid., p. 4436; J. G. Mitchell, L. Pearson, S. Dillon, ibid. 62, 3716 (1996); G. M. Barbara and J. G. Mitchell, ibid., p. 3985.
21. T. Fenchel, Microbiology 140, 3109 (1994); J. G. Mitchell et al., Appl. Environ. Microbiol. 61, 877 (1995); J. G. Mitchell, L. Pearson, S. Dillon, K. Kantalis, ibid., p. 4436; J. G. Mitchell, L. Pearson, S. Dillon, ibid. 62, 3716 (1996); G. M. Barbara and J. G. Mitchell, ibid., p. 3985.
22. Samples of seawater were enriched with 0.02% tryptic soy broth and left overnight, after which the culture contained strains of highly motile bacteria. Samples were sandwiched between a slide and cover slip after the addition of cells from a pure culture of Pavlova lutheri. A ring of bacteria around the air-water interface of the chamber indicated near anoxia inside the chamber.
23. P. A. Wheeler, in Nitrogen in the Marine Environment, E. J. Carpenter and D. G. Capone, Eds. (Academic Press, New York, 1983), p. 309.
24. F. Azam and J. W. Ammerman, in Flows of Energy and Materials in Marine Ecosystems, M. J. R. Fasham, Ed. (Plenum, New York, 1984), p. 345.
25. N. Blackburn, T. Fenchel, J. Mitchell, data not shown.
26. J. D. Bowen and K. D. Stolzenbach, J. Fluid Mech. 236, 95 (1992).
27. L. Karp-Boss, E. Boss, P. A. Jumars, Oceanogr. Mar. Biol. Annu. Rev. 34, 71 (1996).
28. H. C. Berg and D. A. Brown, Nature 239, 500 (1972).
29. -1. The resulting digital film-strips were analyzed frame by frame for trajectories of movement by LabTrack (DiMedia, Kvistgaard, Denmark). Objects moving out of the plane of focus resulted in short tracks and were filtered out of the set of track vectors. Tumbles were detected at points where changes in trajectory angle between two video frames exceeded 1 rad. Runs were defined as periods between tumbles.
30. -1 was introduced. Swimming velocities and mean run durations were acquired from tracks of live cells.
31. -1. The inverse square-root dependency on the distance r from the source was introduced instead of inverse proportionality (3) to more closely approximate its shape in the flat chamber.
32. -1, τ = 0.4 s.
33. The mass flow of a solute at concentration C with diffusion coefficient D toward a sphere of radius a is 4πaDC.
34. We thank the Swedish Foundation for International Cooperation in Research and Higher Education (STINT), the Danish National Research Council (SNF), the Australian Research Council, and Flinders University for support of this study.