Inactivation of a defined active site in the mouse 20S proteasome complex enhances major histocompatibility complex class I antigen presentation of a murine cytomegalovirus protein

Journal of Experimental Medicine

Volumn 187, Issue 10, 1998, Pages 1641-1646

  Schmidtke, Gunter ^a   Eggers, Maren ^b   Ruppert, Thomas ^b   Groettrup, Marcus ^a,c   Koszinowski, Ulrich H ^b   Kloetzel, Peter M ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

^b UNIVERSITY OF MUNICH   (Germany)

^c CANTONAL HOSPITAL ST GALLEN   (Switzerland)

Author keywords

Active site; Antigen processing; Low molecular weight protein 2 T1A; Mutation; Proteosome

Indexed keywords

MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; PROTEASOME; VIRUS PROTEIN;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ANTIGEN EXPRESSION; ANTIGEN PRESENTATION; ARTICLE; CELL SURFACE; CYTOLYSIS; CYTOMEGALOVIRUS; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING;

ANIMALS; ANTIGEN PRESENTATION; ANTIGENS, VIRAL; BINDING SITES; CELL LINE; CYSTEINE ENDOPEPTIDASES; HISTOCOMPATIBILITY ANTIGENS CLASS I; MICE; MICE, INBRED BALB C; MULTIENZYME COMPLEXES; MUROMEGALOVIRUS; PROTEASOME ENDOPEPTIDASE COMPLEX;

EID: 0032543205     PISSN: 00221007     EISSN: None     Source Type: Journal    
DOI: 10.1084/jem.187.10.1641     Document Type: Article

References (27)

1. Rock, K.L., G. Gramm, L. Rothstein, K. Clark, R. Stein, L. Dick, D. Hwang, and A.L. Goldberg. 1994. Inhibitors of the proteasome block the degradation of most cell proteins and the generation of peptides presented on MHC class I molecules. Cell. 78:761-771.
2. Coux, O., K. Tanaka, and A.L. Goldberg. 1996. Structure and functions of the 20S and 26S proteasomes. Annu. Rev. Biochem. 65:801-847.
3. Groettrup, M., A. Soza, U. Kuckelkorn, and P.-M. Kloetzel. 1996. Peptide antigen production by the proteasome: complexity provides efficiency. Immunol. Today. 17:429-435.
4. Lehner, P.J., and P. Cresswell. 1996. Processing and delivery of peptides presented by the MHC class I molecules. Curr. Opin. Immunol. 8:59-67.
5. Seemüller, E., A. Lupas, D. Stock, J. Löwe, R. Huber, and W. Baumeister. 1995. Proteasome from Thermoplasma acidophilum: a threonine protease. Science. 268:579-582.
6. Grziwa, A., W. Baumeister, B. Dahlmann, and F. Kopp. 1991. Localization of subunits in proteasomes from Thermoplasma acidophilum by immunoelectron microscopy. FEBS Lett. 290: 186-190.
7. Groll, M., L. Ditzel, J. Löwe, D. Stock, M. Bochtler, H.D. Bartunik, and R. Huber. 1997. Structure of the 20S proteasome from yeast at 2.4 Å resolution. Nature. 386:463-471.
8. Früh, K., M. Gossen, K. Wang, H. Bujard, P.A. Peterson, and Y. Yang. 1994. Displacement of the housekeeping proteasome subunits by MHC-encoded LMPs: a newly discovered mechanism for modulating the multicatalytic proteinase complex. EMBO (Eur. Mol. Biol. Organ.) J. 13:3236-3244.
9. Groettrup, M., R. Kraft, S. Kostka, S. Standera, R. Stohwasser, and P.M. Kloetzel. 1996. A third interferon-gamma induced subunit exchange in the 20S proteasome. Eur. J. Immunol. 26: 863-869.
10. Kelly, A., S.H. Powis, R. Glynne, E. Radley, S. Beck, and J. Trowsdale. 1991. Second proteasome-related gene in the human MHC class II region. Nature. 353:667-668.
11. Ortiz-Navarrete, V., A. Seelig, M. Gernold, S. Frentzel, P.-M. Kloetzel, and G.J. Hämmerling. 1991. Subunit of the 20S proteasome (multicatalytic proteinase) encoded by the major histocompatibility complex. Nature. 353:662-664.
12. Groettrup, M., S. Standera, R. Stohwasser, and P.-M. Kloetzel. 1997. The subunits MECL-1 and LMP2 are mutually required for incorporation into the 20S proteasome. Proc. Natl. Acad. Sci. USA. 94:8970-8975.
13. Gaczynska, M., K.L. Rock, and A.L. Goldberg. 1993. Gamma-interferon and expression of MHC genes regulate peptide hydrolysis by proteasomes. Nature. 365:264-267.
14. Kuckelkorn, U., S. Frentzel, R. Kraft., S. Kostka, M. Groettrup, and P.-M. Kloetzel. 1995. Incorporation of major histocompatibility complex encoded subunits LMP2 and LMP7 changes the quality of the 20S proteasome polypeptide processing products independent of interteron-gamma. Eur. J. Immunol. 25:2605-2611.
15. Boes, B., H. Hengel, T. Ruppert, G. Multhaup, U.H. Koszinowski, and P.-M. Kloetzel. 1994. Interteron-γ stimulation modulates the proteolytic activity and the cleavage site preference of 20S mouse proteasomes. J. Exp. Med. 179:901-909.
16. Fehling, H.J., W. Swat, C. Laplace, R. Kühn, K. Rajewsky, U. Müller, and H. Von Boehmer. 1994. MHC class I expression in mice lacking the proteasome subunit LMP-7. Science. 265:1234-1237.
17. Van Kaer, L., P.G. Ashton-Rickardt, M. Eichelberger, M. Gaczynska, K. Nagashima, K.L. Rock, A.L. Goldberg, P.C. Doherty, and S. Tonegawa. 1994. Altered peptidase and viral specific T cell response in LMP2 mutant mice. Immunity. 1: 533-541.
18. Cerundolo, V., A. Benham, V. Braud, S. Mukherjee, K. Gould, B. Macino, J. Neefjes, and A. Townsend. 1997. The proteasome specific inhibitor lactacystin blocks presentation of cytotoxic T lymphocyte epitopes in human and murine cells. Eur. J. Immunol. 27:336-341.
19. Fenteany, G., R.F. Standaert, W.S. Lane, S. Choi, E.J. Corey, and S.L. Schreiber. 1995. Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin. Science. 268:726-731.
20. Dick, L.R., A.A. Cruikshank, L. Grenier, F.D. Melandri, S.L. Nunes, and R.L. Stein. 1996. Mechanistic studies on the inactivation of the proteasome by lactacystin: a central role for clasto-lactacystin beta-lactone. J. Biol. Chem. 271:7273-7276.
21. Schmidtke, G., R. Kraft, S. Kostka, P. Henklein, C. Frömmel, J. Löwe, R. Huber, P.-M. Kloetzel, and M. Schmidt. 1996. Analysis of mammalian 20S proteasome biogenesis: the maturation of β subunits is an ordered two step mechanism involving autocatalysis. EMBO (Eur. Mol. Biol. Organ.) J. 15: 6887-6898.
22. Hengel, H., P. Lucin, S. Jonjic, T. Ruppert, and U.H. Koszinowski. 1994. Restoration of cytomegalovirus antigen presentation by gamma Interferon combats viral escape. J. Virol. 68:289-297.
23. Maniatis, T., J. Sambrook, and E.F. Fritsch. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 1.1-18.8.
24. Ljunggren, H.G., N.J. Stam, C. Ohlen, J.J. Neefjes, P. Hoglund, M.T. Heemels, J. Bastin, T.N. Schumacher, A. Townsend, K. Karre, et al. 1990. Empty MHC class I molecules come out in the cold. Nature. 346:476-480.
25. Schumacher, T.N., M.T. Heemels, J.J. Neefjes, W.M. Kast, C.J. Melief, and H.-L. Ploegh. 1990. Direct binding of peptide to empty MHC class I molecules on intact cells and in vitro. Cell. 62:563-567.
26. Del Val, M., H.J. Volkmer, J. Rothbard, S. Jonjic, M. Messerle, J. Schickedanz, M. Reddehase, and U.H. Koszinowski. 1988. Molecular basis for cytolytic T-lymphocyte recognition of the murine cytomegalovirus immediate early protein pp89. J. Virol. 62:3965-3972.
27. Eleuteri, A.M., R.A. Koansk, C. Cardzo, and M. Orlowski. 1997. Bovine spleen multicatalytic proteinase (proteasome): replacement of X, Y, and Z subunits by LMP7, LMP2, and MECL1 and changes in properties and specificity. J. Biol. Chem. 272:11824-11831.