βGal(1→s-4)βGlcNac-oR: A galactosidase-stable substrate for α(1→3)fucosyltransferase

Bioorganic and Medicinal Chemistry Letters

Volumn 8, Issue 22, 1998, Pages 3199-3202

  Ding, Yili ^a   Hindsgaul, Ole ^a   Li, Hong ^a   Palcic, Monica M ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

1',4 THIO N ACETYLLACTOSAMINE; AMINE; FUCOSYLTRANSFERASE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM ADHERENCE; CARBOHYDRATE SYNTHESIS; CELL ADHESION; CHEMICAL STRUCTURE; ENZYME SUBSTRATE COMPLEX; MOLECULAR RECOGNITION; SIGNAL TRANSDUCTION; SYNTHESIS;

BACTERIA (MICROORGANISMS);

EID: 0032541987     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(98)00552-6     Document Type: Article

References (10)

1. 1. Schmidt, R. R. Adv. Carbohydr. Chem. Biochem. 1994, 50, 21.
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3. 3. Hakomori, S. Adv. Cancer Res. 1989, 52, 257.
4. 4. Zhang, Y.; Le, X.; Dovichi, N. J.; Compston, C. A.; Palcic, M. M.; Diedrich, P.; Hindsgaul, O. Anal. Biochem. 1994, 227, 368.
5. 5. Yuasa, H.; Hindsgaul, O.; Palcic, M. M. J. Amer. Chem. Soc. 1992, 114, 5891.
6. 6. Defaye, J; Gelas, J. In Study in Natural Products Chemistry, Atta-ur-Rahman Ed. Elsevier: Amsterdam, 1991, 8, 315.
7. 7. Qian, X.; Hindsgaul, O.; Li, H.; Palcic, M. M. J. Amer. Chem. Soc. 1998, 120, 2184 and references cited therein.
8. 8. Palcic, M. M.; Heerze, L. D.; Pierce, M.; Hindsgaul, O. Glycoconjugate J. 1988, 5, 49.
9. 2, 0.2% BSA and 25% glycerol was incubated at 37 °C for 3 days. Additional GDP-Fuc (1.0 mg) was added after 24 h and again after 48 h. The reaction was stopped by the addition of 8 mL of water and the sample was isolated by loading the mixture onto two sequential C-18 Sep-Pak cartridges (Waters). The cartridges were washed with water to remove enzyme and unreacted nucleotide donor and the product was eluted with methanol to give trisaccharide 10.
10. +.