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Volumn 280, Issue 5362, 1998, Pages 443-446

Control of alternative splicing of potassium channels by stress hormones

Author keywords

[No Author keywords available]

Indexed keywords

CORTICOSTERONE; CORTICOTROPIN; MEMBRANE PROTEIN; PHENYLETHANOLAMINE N METHYLTRANSFERASE; POTASSIUM CHANNEL; SLO PROTEIN; UNCLASSIFIED DRUG;

EID: 0032540395     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.280.5362.443     Document Type: Article
Times cited : (262)

References (34)
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    • IMMUCHEM Double Antibody Corticosterone RIA kit (ICN, Irvine, CA) was used by the veterinary diagnostic lab at Cornell University. Corticosterone was extracted from ground adrenals with 0.5 ml of ethyl acetate at room temperature and spun at 11,000 rpm for 5 min, and the upper phase was saved, vacuum dried, and resuspended in 400 μl of resuspension buffer with 5% ethanol
    • IMMUCHEM Double Antibody Corticosterone RIA kit (ICN, Irvine, CA) was used by the veterinary diagnostic lab at Cornell University. Corticosterone was extracted from ground adrenals with 0.5 ml of ethyl acetate at room temperature and spun at 11,000 rpm for 5 min, and the upper phase was saved, vacuum dried, and resuspended in 400 μl of resuspension buffer with 5% ethanol.
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    • note
    • Plasmid constructs for cRNA expression in Xenopus oocytes were devised by modification of a mouse Slo (mSlo) expression construct (5). Rat adrenal RT-PCR products corresponding to ZERO, STREX-1, and STREX-2 variants were made with primers rSlo3 and a downstream primer with ah internal Xho I site (sequence: 5′-ACCGCTCGAGCTCTGGCGTGGCTCCTCCTG-3′) and cloned into unique Hind III and Xho I sites in the mSlo vector. Restriction analysis and sequencing were used to confirm constructs. mSlo differs from rSlo outside the rat insert at only 4 of 716 residues. Rasmids were linearized with Sal I and transcribed with Ambion T3 mMESSAGE kit.
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    • Patch clamp solutions were potassium methanesulfonate based (pH 7.0). Free calcium concentrations were determined with a calcium-selective electrode (Orion, Beverly, MA). Procedure and solutions were as described by D. P. McCobb et al. [Am. J. Physiol. 269, H767 (1995)]. A List EPC-7 patch clamp with ITC-16 data interface (Instrutech, NY) and Pulse software (HEKA, Lambrecht, Germany) were used for data acquisition.
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    • We thank R. Harris-Warrick, D. Baro, R. Knapp, A. Bass, and R. Hoy for helpful discussions. Supported by the American Heart Association
    • We thank R. Harris-Warrick, D. Baro, R. Knapp, A. Bass, and R. Hoy for helpful discussions. Supported by the American Heart Association.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.