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Volumn 280, Issue 5362, 1998, Pages 443-446

Control of alternative splicing of potassium channels by stress hormones

Author keywords

[No Author keywords available]

Indexed keywords

CORTICOSTERONE; CORTICOTROPIN; MEMBRANE PROTEIN; PHENYLETHANOLAMINE N METHYLTRANSFERASE; POTASSIUM CHANNEL; SLO PROTEIN; UNCLASSIFIED DRUG;

EID: 0032540395     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.280.5362.443     Document Type: Article
Times cited : (263)

References (34)
  • 1
    • 0025305508 scopus 로고
    • M. E. Ross et al., J. Neurosci. 10, 520 (1990); S. N. Ebert et al., J. Biol. Chem. 269, 20885 (1994).
    • (1990) J. Neurosci. , vol.10 , pp. 520
    • Ross, M.E.1
  • 2
    • 0028101465 scopus 로고
    • M. E. Ross et al., J. Neurosci. 10, 520 (1990); S. N. Ebert et al., J. Biol. Chem. 269, 20885 (1994).
    • (1994) J. Biol. Chem. , vol.269 , pp. 20885
    • Ebert, S.N.1
  • 3
    • 0029861391 scopus 로고    scopus 로고
    • E. S. Levitan, K. M. Hershman, T. G. Sherman, K. Takimoto, Neuropharmacology 35, 1001 (1996); K. Takimoto and E. S. Levitan, Circ. Res. 75, 1 (1994); K. Takimoto, A. F. Fomina, R. Gealy, J. S. Trimmer, E. S. Levitan, Neuron 11, 359 (1993); B. Attardi, K. Takimoto, R. Gealy, C. Severns, E. S. Levitan, Receptors Channels 1, 287 (1993).
    • (1996) Neuropharmacology , vol.35 , pp. 1001
    • Levitan, E.S.1    Hershman, K.M.2    Sherman, T.G.3    Takimoto, K.4
  • 4
    • 85136544370 scopus 로고
    • E. S. Levitan, K. M. Hershman, T. G. Sherman, K. Takimoto, Neuropharmacology 35, 1001 (1996); K. Takimoto and E. S. Levitan, Circ. Res. 75, 1 (1994); K. Takimoto, A. F. Fomina, R. Gealy, J. S. Trimmer, E. S. Levitan, Neuron 11, 359 (1993); B. Attardi, K. Takimoto, R. Gealy, C. Severns, E. S. Levitan, Receptors Channels 1, 287 (1993).
    • (1994) Circ. Res. , vol.75 , pp. 1
    • Takimoto, K.1    Levitan, E.S.2
  • 5
    • 0027328430 scopus 로고
    • E. S. Levitan, K. M. Hershman, T. G. Sherman, K. Takimoto, Neuropharmacology 35, 1001 (1996); K. Takimoto and E. S. Levitan, Circ. Res. 75, 1 (1994); K. Takimoto, A. F. Fomina, R. Gealy, J. S. Trimmer, E. S. Levitan, Neuron 11, 359 (1993); B. Attardi, K. Takimoto, R. Gealy, C. Severns, E. S. Levitan, Receptors Channels 1, 287 (1993).
    • (1993) Neuron , vol.11 , pp. 359
    • Takimoto, K.1    Fomina, A.F.2    Gealy, R.3    Trimmer, J.S.4    Levitan, E.S.5
  • 6
    • 0027145792 scopus 로고
    • E. S. Levitan, K. M. Hershman, T. G. Sherman, K. Takimoto, Neuropharmacology 35, 1001 (1996); K. Takimoto and E. S. Levitan, Circ. Res. 75, 1 (1994); K. Takimoto, A. F. Fomina, R. Gealy, J. S. Trimmer, E. S. Levitan, Neuron 11, 359 (1993); B. Attardi, K. Takimoto, R. Gealy, C. Severns, E. S. Levitan, Receptors Channels 1, 287 (1993).
    • (1993) Receptors Channels , vol.1 , pp. 287
    • Attardi, B.1    Takimoto, K.2    Gealy, R.3    Severns, C.4    Levitan, E.S.5
  • 10
    • 0028579516 scopus 로고
    • A. Butler, S. Tsunoda, D. P. McCobb, A. Wei, L. Salkoff, Science 261, 221 (1993); J. Tseng-Crank et al., Neuron 13, 1315 (1994).
    • (1994) Neuron , vol.13 , pp. 1315
    • Tseng-Crank, J.1
  • 11
    • 0026794812 scopus 로고
    • J. P. Adelman et al., Neuron 9, 209 (1992); N. S. Atkinson, G. A. Robertson, B. Ganetzky, Science 253, 551 (1991).
    • (1992) Neuron , vol.9 , pp. 209
    • Adelman, J.P.1
  • 14
    • 0027956835 scopus 로고
    • M. K. Stachowiak, R. J. Rigual, P. H. K. Lee, O. H. Viveros, J. S. Hong, Mol. Brain Res. 3, 275 (1988); E. Viskupic et al., J. Neurochem. 63, 808 (1994).
    • (1994) J. Neurochem. , vol.63 , pp. 808
    • Viskupic, E.1
  • 15
    • 2642591069 scopus 로고    scopus 로고
    • J. Xie, P. Lappas, W. Yao, E. Carpenter-Hyland, D. P. McCobb, Soc. Neurosci. Abstr. 22, 402 (1996). For calibrated RT-PCR, different amounts of calibrator template cRNA {constructed as per S. Diviacco et al. [Gene 122, 313 (1992)]} were added to identical 12.5-ng samples of tissue RNA. Relative PCR product band intensity was measured with BIT Image 1.55 (Digital Imagers, Elburn, IL). We measured the native template by extrapolating to the calibrator template amount yielding equivalent product bands, with corrections for length effects on amplification efficiency and band intensity.
    • (1996) Soc. Neurosci. Abstr. , vol.22 , pp. 402
    • Xie, J.1    Lappas, P.2    Yao, W.3    Carpenter-Hyland, E.4    McCobb, D.P.5
  • 16
    • 0027086910 scopus 로고
    • J. Xie, P. Lappas, W. Yao, E. Carpenter-Hyland, D. P. McCobb, Soc. Neurosci. Abstr. 22, 402 (1996). For calibrated RT-PCR, different amounts of calibrator template cRNA {constructed as per S. Diviacco et al. [Gene 122, 313 (1992)]} were added to identical 12.5-ng samples of tissue RNA. Relative PCR product band intensity was measured with BIT Image 1.55 (Digital Imagers, Elburn, IL). We measured the native template by extrapolating to the calibrator template amount yielding equivalent product bands, with corrections for length effects on amplification efficiency and band intensity.
    • (1992) Gene , vol.122 , pp. 313
    • Diviacco, S.1
  • 17
    • 2642686731 scopus 로고    scopus 로고
    • Sprague-Dawley rats (Charles River) were hypophy-sectomized with the parapharyngeal approach to the occipitosphenoid suture. After trephination, the hypophysis was removed by aspiration. Hypophysectomized rats received 5% sucrose water. Marked reductions in adrenal cortical volume and rat body weight at death, compared with age- and sex-matched rats, indicated successful hypophysectomy
    • Sprague-Dawley rats (Charles River) were hypophy-sectomized with the parapharyngeal approach to the occipitosphenoid suture. After trephination, the hypophysis was removed by aspiration. Hypophysectomized rats received 5% sucrose water. Marked reductions in adrenal cortical volume and rat body weight at death, compared with age- and sex-matched rats, indicated successful hypophysectomy.
  • 20
    • 0010624756 scopus 로고    scopus 로고
    • 22 (Gibco BRL). One microliter of RT product was used in a 25-μl PCR for 30 cycles at 94°C, 30-s denaturation; 55°C. 30-s annealing; and 72°C, 30-s extension. Five microliters of product was run on a 2% agarose gel stained with ethidium bromide.
    • (1997) Soc. Neurosci. Abstr. , vol.23 , pp. 309
    • Xie, J.1    McCobb, D.P.2
  • 21
    • 2642631862 scopus 로고    scopus 로고
    • ACTH was in gel form (Rhone Poulenc Rorer Pharmaceutical, Collegeville, PA)
    • ACTH was in gel form (Rhone Poulenc Rorer Pharmaceutical, Collegeville, PA).
  • 23
    • 0014027532 scopus 로고
    • R. E. Coupland, The Natural History of the Chromaffin Cell (Longmans, London, 1965); R. J. Wurtman and J. Axelrod, J. Biol. Chem. 241, 2301 (1966).
    • (1966) J. Biol. Chem. , vol.241 , pp. 2301
    • Wurtman, R.J.1    Axelrod, J.2
  • 24
    • 2642589114 scopus 로고    scopus 로고
    • IMMUCHEM Double Antibody Corticosterone RIA kit (ICN, Irvine, CA) was used by the veterinary diagnostic lab at Cornell University. Corticosterone was extracted from ground adrenals with 0.5 ml of ethyl acetate at room temperature and spun at 11,000 rpm for 5 min, and the upper phase was saved, vacuum dried, and resuspended in 400 μl of resuspension buffer with 5% ethanol
    • IMMUCHEM Double Antibody Corticosterone RIA kit (ICN, Irvine, CA) was used by the veterinary diagnostic lab at Cornell University. Corticosterone was extracted from ground adrenals with 0.5 ml of ethyl acetate at room temperature and spun at 11,000 rpm for 5 min, and the upper phase was saved, vacuum dried, and resuspended in 400 μl of resuspension buffer with 5% ethanol.
  • 25
    • 2642602198 scopus 로고    scopus 로고
    • note
    • Plasmid constructs for cRNA expression in Xenopus oocytes were devised by modification of a mouse Slo (mSlo) expression construct (5). Rat adrenal RT-PCR products corresponding to ZERO, STREX-1, and STREX-2 variants were made with primers rSlo3 and a downstream primer with ah internal Xho I site (sequence: 5′-ACCGCTCGAGCTCTGGCGTGGCTCCTCCTG-3′) and cloned into unique Hind III and Xho I sites in the mSlo vector. Restriction analysis and sequencing were used to confirm constructs. mSlo differs from rSlo outside the rat insert at only 4 of 716 residues. Rasmids were linearized with Sal I and transcribed with Ambion T3 mMESSAGE kit.
  • 26
    • 0028894608 scopus 로고
    • Patch clamp solutions were potassium methanesulfonate based (pH 7.0). Free calcium concentrations were determined with a calcium-selective electrode (Orion, Beverly, MA). Procedure and solutions were as described by D. P. McCobb et al. [Am. J. Physiol. 269, H767 (1995)]. A List EPC-7 patch clamp with ITC-16 data interface (Instrutech, NY) and Pulse software (HEKA, Lambrecht, Germany) were used for data acquisition.
    • (1995) Am. J. Physiol. , vol.269
    • McCobb, D.P.1
  • 34
    • 2642603299 scopus 로고    scopus 로고
    • We thank R. Harris-Warrick, D. Baro, R. Knapp, A. Bass, and R. Hoy for helpful discussions. Supported by the American Heart Association
    • We thank R. Harris-Warrick, D. Baro, R. Knapp, A. Bass, and R. Hoy for helpful discussions. Supported by the American Heart Association.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.