Mechanism of the α-complementation reaction of E. coli β-galactosidase deduced from fluorescence correlation spectroscopy measurements

Biophysical Chemistry

Volumn 75, Issue 2, 1998, Pages 151-160

  Meyer Almes, F J ^a,c   Wyzgol, K ^a   Powell, M J ^b  

^a EVOTEC BioSystems GmbH   (Germany)

^b F HOFFMANN LA ROCHE LTD   (Switzerland)

^c EVOTEC Analytical Systems GmbH   (Germany)

Author keywords

Galactosidase; Association kinetics; CEDIA(TM); Fluorescence correlation spectroscopy; Reaction mechanism

Indexed keywords

BACTERIAL ENZYME; BETA GALACTOSIDASE;

ARTICLE; COMPLEX FORMATION; ENZYME ACTIVITY; ESCHERICHIA COLI; FLUORESCENCE SPECTROSCOPY; FREEZE DRYING; PRIORITY JOURNAL; REACTION ANALYSIS;

BETA-GALACTOSIDASE; BIOPOLYMERS; ESCHERICHIA COLI; KINETICS; PROTEIN BINDING; PROTEIN DENATURATION; SPECTROMETRY, FLUORESCENCE;

EID: 0032539003     PISSN: 03014622     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0301-4622(98)00203-8     Document Type: Article

References (17)

1. Ullman A., Jacob F., Monod J. Mol. Biol. 24:1967;339.
2. C. Gallagher, Ph.D. thesis, University of Calgary, 1994.
3. Khanna P.L., Dworschack R.T., Manning W.B., Harris J.D. Clin. Chim. Acta. 185:1989;231.
4. Henderson D.R., Friedman S.B., Harris J.D., Manning W.B., Zoccoli M.A. Clin. Chem. 32/9:1986;1637.
5. Coty W.A., Loor R., Powell M.J., Khanna P.L. J. Clin. Immunoassay. 17:1994;144.
6. Langley K.E., Fowler A.V., Zabin I. J. Biol. Chem. 250:1975;2587.
7. Smoluchowski M.V. Z. Phys. Chem. 92:1917;129.
8. Fowler A., Zabin I. J. Biol. Chem. 252:1978;5521.
9. Jacobson R.H., Zhang X.-H., Dubose R.F., Matthews B.W. Nature. 369:1994;761.
10. G. Wanner, in: D.F. Griffith, G.A. Watson (Eds.), Numerical Analysis, Pitman Research Notes in Mathematics, vol. 170, Harlow, Essex, UK, 1987.
11. M. Eigen, in: Investigation of Rates and Mechanism of Reactions, Technique of Organic Chemistry, vol. VIII, part 11, Wiley-Interscience, New York, 1963, p. 895.
12. Rigler R., Mets Ü. Soc. Photo Opt. Instrumen. Eng. 1921:1992;239.
13. Widengren J., Rigler R., Mets Ü. J. Fluoresc. 4:1994;255.
14. Schwille P., Meyer-Almes F.-J., Rigler R. Biophys. J. 72:1997;1878.
15. J. Widengren, Ph.D. thesis, Karolinska Institute Stockholm, 1990.
16. Langley K.E., Villarejo M.R., Fowler A.V., Zamenhof P.J., Zabin I. Proc. Natl. Acad. Sci. U.S.A. 72:1975;1254.
17. Ullman A. BioEssays. 14:1992;201.