Tim9p, an essential partner subunit of Tim10p for the import of mitochondrial carrier proteins

EMBO Journal

Volumn 17, Issue 22, 1998, Pages 6477-6486

  Koehler, Carla M ^a   Merchant, Sabeeha ^a,b   Oppliger, Wolfgang ^a   Schmid, Karl ^a   Jarosch, Ernst ^a,c   Dolfini, Luisita ^a   Junne, Tina ^a   Schatz, Gottfried ^a   Tokatlidis, Kostas ^a,d  

^a UNIVERSITY OF BASEL   (Switzerland)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^d UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

Extragenic suppression; Mitochondrial protein import; Saccharomyces cerevisiae; TIM complex

Indexed keywords

CARRIER PROTEIN; CYSTEINE; PROTEIN SUBUNIT; SERINE;

AMINO ACID SEQUENCE; ARTICLE; CHROMATOGRAPHY; CONTROLLED STUDY; FUNGUS GROWTH; IMMUNOPRECIPITATION; MITOCHONDRIAL MEMBRANE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN CROSS LINKING; PROTEIN PURIFICATION; PROTEIN TRANSPORT; SEQUENCE HOMOLOGY; TEMPERATURE SENSITIVE MUTANT;

FUNGI; SACCHAROMYCES CEREVISIAE;

EID: 0032538975     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.22.6477     Document Type: Article

References (40)

1. Cline, K. and Henry, R. (1996) Import and routing of nucleus-encoded chloroplast proteins. Annu. Rev. Cell Dev. Biol., 12, 1-26.
2. Dekker, P.J., Müller, H., Rassow, J. and Pfanner, N. (1996) Characterization of the preprotein translocase of the outer mitochondrial membrane by blue native electrophoresis. Biol. Chem., 377, 535-538.
3. Dietmeier, K., Hönlinger, A., Bomer, U., Dekker, P.J., Eckerskorn, C., Lottspeich, F., Kübrich, M. and Pfanner, N. (1997) Tom5 functionally links mitochondrial preprotein receptors to the general import pore. Nature, 388, 195-200.
4. el Moualij, B., Duyckaerts, C., Lamotte-Brasseur, J. and Sluse, F.E. (1997) Phylogenetic classification of the mitochondrial carrier family of Saccharomyces cerevisiae. Yeast, 13, 573-581.
5. Fujiki, Y., Hubbard, A.L., Fowler, S. and Lazarow, P.B. (1982) Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum. J. Cell Biol., 93, 97-102.
6. Gietz, R.D. and Sugino, A. (1988) New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene, 74, 527-534.
7. Click, B.S. and Pon, L. (1995) Isolation of highly purified mitochondria from S.cerevisiae. Methods Enzymol., 260, 213-233.
8. Glick, B.S., Wachter, C. and Schatz, G. (1991) Protein import into mitochondria: two systems acting in tandem. Trends Cell. Biol., 1, 99-103.
9. Glick, B.S., Brandt, A., Cunningham, K., Müller, S., Hallberg, R.L. and Schatz, G. (1992) Cytochromes c1 and b2 are sorted to the intermembrane space of yeast mitochondria by a stop-transfer mechanism. Cell, 69, 809-822.
10. Guthrie, C. and Fink, G.R. (1991) Guide to Yeast Genetics and Molecular Biology. Academic Press, San Diego, CA.
11. High, S., Henry, R., Mould, R.M., Valent, Q., Meacock, S., Cline, K., Gray, J.C. and Luirink, J. (1997) Chloroplast SRP54 interacts with a specific subset of thylakoid precursor proteins. J. Biol. Chem., 272, 11622-11628.
12. Hines, V., Brandt, A., Griffiths, G., Horstmann, H., Brütsch, H. and Schatz, G. (1990) Protein import into yeast mitochondria is accelerated by the outer membrane protein MAS70. EMBO J., 9, 3191-3200.
13. Jarosch, E., Tuller, G., Daum, G., Waldherr, M., Voskova, A. and Schweyen, R.J. (1996) Mrs5p, an essential protein of the mitochondrial intermembrane space, affects protein import into yeast mitochondria. J. Biol. Chem., 271, 17219-17225.
14. Kerscher, O., Holder, J., Srinivasan, M., Leung, R.S. and Jensen, R.E. (1997) The Tim54p-Tim22p complex mediates insertion of proteins into the mitochondrial inner membrane. J. Cell Biol., 139, 1663-1675.
15. Koehler, C.M., Jarosch, E., Tokatlidis, K., Schmid, K., Schweyen, R.J. and Schatz, G. (1998) Import of mitochondrial carriers mediated by essential proteins of the intermembrane space. Science, 279, 369-373.
16. Komiya, T., Rospert, S., Koehler, C., Looser, R., Schatz, G. and Mihara, K. (1998) Interaction of mitochondrial targeting signals with acidic receptor domains along the protein import pathway: evidence for the 'acid chain' hypothesis. EMBO J., 17, 3886-3898.
17. Kübrich, M., Rassow, J., Voos, W., Pfanner, N. and Hönlinger, A. (1998) The import route of ADP/ATP carrier into mitochondria separates from the general import pathway of cleavable preproteins at the trans side of the outer membrane. J. Biol. Chem., 273, 16374-16381.
18. Leonhard, K., Herrmann, J.M., Stuart, R.A., Mannhaupt, G., Neupert, W. and Langer, T. (1996) AAA proteases with catalytic sites on opposite membrane surfaces comprise a proteolytic system for the ATP-dependent degradation of inner membrane proteins in mitochondria. EMBO J., 15, 4218-4229.
19. Li, X., Henry, R., Yuan, J., Cline, K. and Hoffman, N.E. (1995) A chloroplast homologue of the signal recognition particle subunit SRP54 is involved in the posttranslational integration of a protein into thylakoid membranes. Proc. Natl Acad. Sci. USA, 92, 3789-3793.
20. Neupert, W. (1997) Protein import into mitochondria. Annu. Rev. Biochem., 66, 863-917.
21. Palmieri, F., Bisaccia, F., Capobianco, L., Dolce, V., Fiermonte, G., Iacobazzi, V., Indiveri, C. and Palmieri, L. (1996) Mitochondrial metabolite transporters. Biochim. Biophys. Acta, 1275, 127-132.
22. Payan, L.A. and Cline, K. (1991) A stromal protein factor maintains the solubility and insertion competence of an imported thylakoid membrane protein. J. Cell Biol., 112, 603-613.
23. Pfanner, N. (1998) Mitochondrial import: crossing the aqueous intermembrane space. Curr. Biol., 8, R262-R265.
24. Pfanner, N. and Meijer, M. (1997) The Tom and Tim machine. Curr. Biol., 7, 100-103.
25. Pfanner, N., Hoeben, P., Tropschug, M. and Neupert, W. (1987) The carboxyl-terminal two-thirds of the ADP/ATP carrier polypeptide contains sufficient information to direct translocation into mitochondria. J. Biol. Chem., 262, 14851-14854.
26. Pfanner, N. et al. (1996) Uniform nomenclature for the protein transport machinery of the mitochondrial membranes. Trends Biochem. Sci., 21, 51-52.
27. Rospert, S. and Schatz, G. (1998) Protein translocation into mitochondria. In Celis, J.E. (ed.), Cell Biology: A Laboratory Handbook. Vol. 2. Academic Press, San Diego, CA, pp. 277-285.
28. Ryan, K.R. and Jensen, R.E. (1995) Protein translocation across mitochondrial membranes: what a long, strange trip it is. Cell, 83, 517-519.
29. Schägger, H. and von Jagow, G. (1987) Tricine-sodium dodecyl sulfate -polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem., 166, 368-379.
30. Schägger, H. and von Jagow, G. (1991) Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal. Biochem., 199, 223-231.
31. Schägger, H., Cramer, W.A. and von Jagow, G. (1994) Analysis of molecular masses and oligomeric states of protein complexes by blue native electrophoresis and isolation of membrane protein complexes by two-dimensional native electrophoresis. Anal. Biochem., 217, 220-230.
32. Schatz, G. (1997) Just follow the acid chain. Nature, 388, 121-122.
33. Schatz, G. and Dobberstein, B. (1996) Common principles of protein translocation across membranes. Science, 271, 1519-1526.
34. Schuenemann, D., Gupta, S., Persello-Cartieaux, F., Klimyuk, V.I., Jones, J.D.G., Nussaume, L. and Hoffman, N.E. (1998) A novel signal recognition particle targets light-harvesting proteins to the thylakoid membranes. Proc. Natl Acad. Sci. USA, 95, 10312-10316.
35. Sikorski, R.S. and Hieter, P. (1989) A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics, 122, 19-27.
36. Sirrenberg, C., Bauer, M.F., Guiard, B., Neupert, W. and Brunner, M. (1996) Import of carrier proteins into the mitochondrial inner membrane mediated by Tim22. Nature, 384, 582-585.
37. Sirrenberg, C., Endres, M., Folsch, H., Stuart, R.A., Neupert, W. and Brunner, M. (1998) Carrier protein import into mitochondria mediated by the intermembrane proteins Tim10/Mrs11 and Tim12/Mrs5. Nature, 391, 912-915.
38. Smagula, C.S. and Douglas, M.G. (1988) ADP-ATP carrier of Saccharomyces cerevisiae contains a mitochondrial import signal between amino acids 72 and 111. J. Cell. Biochem., 36, 323-327.
39. Wachter, C., Schatz, G. and Glick, B.S. (1994) Protein import into mitochondria: the requirement for external ATP is precursor-specific whereas intramitochondrial ATP is universally needed for translocation into the matrix. Mol. Biol. Cell, 5, 465-474.
40. Weber, E.R., Hanekamp, T. and Thorsness, P.E. (1996) Biochemical and functional analysis of the YME1 gene product, an ATP and zinc-dependent mitochondrial protease from S.cerevisiae. Mol. Biol. Cell, 7, 307-317.