Disruption of target DNA binding in Mu DNA transposition by alteration of position 99 in the Mu B protein

Journal of Molecular Biology

Volumn 275, Issue 2, 1998, Pages 233-243

  Millner, Anders ^a   Chaconas, George ^a  

^a UNIVERSITY OF WESTERN ONTARIO   (Canada)

Author keywords

DNA transposition; Mu B protein; N ethylmaleimide; Strand transfer; Target DNA binding

Indexed keywords

ADENOSINE TRIPHOSPHATE DERIVATIVE; CYSTEINE; N ETHYLMALEIMIDE; SERINE; TYROSINE; VIRUS DNA; VIRUS PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIOPHAGE MU; CONTROLLED STUDY; DNA BINDING; DNA TRANSPOSITION; ENZYME ACTIVATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN MODIFICATION;

DNA VIRUSES; ENTEROBACTERIA PHAGE MU; UNIDENTIFIED BACTERIOPHAGE;

EID: 0032536104     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1997.1446     Document Type: Article

References (45)

1. Adzuma, K. & Mizuuchi, K. (1988). Target immunity of Mu transposition reflects a differential distribution of Mu B protein. Cell, 53, 257-66.
2. Adzuma, K. & Mizuuchi, K. (1989). Interaction of proteins located at a distance along DNA: mechanism of target immunity in the Mu DNA strand-transfer reaction. Cell, 57, 41-47.
3. Adzuma, K. & Mizuuchi, K. (1991). Steady-state kinetic analysis of ATP hydrolysis by the B protein of bacteriophage mu. Involvement of protein oligomerization in the ATPase cycle. J. Biol. Chem. 266, 6159-6167.
4. Allison, R. G. & Chaconas, G. (1992). Role of the A protein-binding sites in the in vitro transposition of mu DNA. A complex circuit of interactions involving the mu ends and the transpositional enhancer. J. Biol. Chem. 267, 19963-19970.
5. Baker, T. A. & Luo, L. (1994). Identification of residues in the Mu transposase essential for catalysis. Proc. Natl Acad. Sci. USA, 91, 6654-6658.
6. Baker, T. A., Mizuuchi, M. & Mizuuchi, K. (1991). MuB protein allosterically activates strand transfer by the transposase of phage Mu. Cell, 65, 1003-1013.
7. Baker, T. A., Mizuuchi, M., Savilahti, H. & Mizuuchi, K. (1993). Division of labor among monomers within the Mu transposase tetramer. Cell, 74, 723-733.
8. Chaconas, G. (1987). Phage Mu (Symonds, N., Toussaint, A., Putte, P. V. D. & Howe, M. M., eds), pp. 137-157, Cold Spring Harbor Laboratory, NY.
9. Chaconas, G., Gloor, G. & Miller, J. L. (1985). Amplification and purification of the bacteriophage Mu encoded B transposition protein. J. Biol. Chem. 260, 2662-2669.
10. Chaconas, G., Lavoie, B. D. & Watson, M. A. (1996). DNA transposition - jumping gene machine, some assembly required. Curr. Biol. 6, 817-820.
11. Craig, N. L. (1995). Unity in transposition reactions. Science, 270, 253-254.
12. Craigie, R. (1996). Quality control in Mu DNA transposition. Cell, 85, 137-140.
13. Craigie, R. & Mizuuchi, K. (1987). Transposition of Mu DNA: joining of Mu to target DNA can be uncoupled from cleavage at the ends of Mu. Cell, 51, 493-501.
14. Deng, W. P. & Nickoloff, J. A. (1992). Site-directed mutagenesis of virtually any plasmid by eliminating a unique site. Anal. Biochem. 200, 81-88.
15. Grindley, N D. F. & Leschziner, A. E. (1995). DNA transposition: from a black box to a color monitor. Cell, 83, 1063-1066.
16. Kim, K., Namgoong, S. Y., Jayaram, M. & Harshey, R. M. (1995). Step-arrest mutants of phage Mu transposase. Implications in DNA-protein assembly, Mu end cleavage, and strand transfer. J. Biol. Chem. 270, 1472-1479.
17. Kruklitis, R., Welty, D. J. & Nakai, H. (1996). Clpx protein of Escherichia coli activates bacteriophage Mu transposase in the strand transfer complex for initiation of Mu DNA synthesis. EMBO J. 15, 935-944.
18. Lavoie, B. D. & Chaconas, G. (1993). Site-specific HU binding in the Mu transpososome: conversion of a sequence-independent DNA-binding protein into a chemical nuclease. Genes Dev. 7, 2510-2519.
19. Lavoie, B. D. & Chaconas, G. (1995). Transposition of phage Mu DNA. Curr. Top. Microbiol. Immunol. 204, 83-99.
20. Lavoie, B. D., Chan, B. S., Allison, R. G. & Chaconas, G. (1991). Structural aspects of a higher order nucleo-protein complex: induction of an altered DNA structure at the Mu-host junction of the Mu type 1 transpososome. EMBO J. 10, 3051-3059.
21. Leung, P. C. & Harshey, R. M. (1991). Two mutations of phage Mu transposase that affect strand transfer or interactions with B protein lie in distinct polypeptide domains. J. Mol. Biol. 219, 189-99.
22. Leung, P. C., Teplow, D. B. & Harshey, R. M. (1989). Interaction of distinct domains in Mu transposase with Mu DNA ends and an internal transpositional enhancer. Nature, 338, 656-658.
23. Levchenko, I., Luo, L. & Baker, T. A. (1995). Disassembly of the Mu transposase tetramer by the Clpx chaperone. Genes Dev. 9, 2399-2408.
24. Maxwell, A., Craigie, R. & Mizuuchi, K. (1987). B protein of bacteriophage Mu is an ATPase that preferentially stimulates intermolecular DNA strand transfer. Proc. Natl Acad. Sci. USA, 84, 699-703.
25. Miller, J. L., Anderson, S. K., Fujita, D. J., Chaconas, G., Baldwin, D. L. & Harshey, R. M. (1984). The nucleotide sequence of the B gene of bacteriophage Mu. Nucl. Acids Res. 12, 8627-8638.
26. Mizuuchi, K. (1992). Transpositional recombination: mechanistic insights from studies of Mu and other elements. Annu. Rev. Biochem. 61, 1011-1051.
27. Mizuuchi, M. & Mizuuchi, K. (1989). Efficient Mu transposition requires interaction of transposase with a DNA sequence at the Mu operator: implications for regulation. Cell, 58, 399-408.
28. Mizuuchi, M., Baker, T. A. & Mizuuchi, K. (1992). Assembly of the active form of the transposase-Mu DNA complex: a critical control point in Mu transposition. Cell, 70, 303-311.
29. Mizuuchi, M., Baker, T. A. & Mizuuchi, K. (1995). Assembly of phage Mu transpososomes - cooperative transitions assisted by protein and DNA scaffolds. Cell, 83, 375-385.
30. Naigamwalla, D. Z. & Chaconas, G. (1997). A new set of transposition intermediates: alternate pathways of target capture preceeding strand transfer. EMBO J. 16, 5227-5234.
31. Nakai, H. & Kruklitis, R. (1995). Disassembly of the bacteriophage Mu transposase for the initiation of Mu DNA replication. J. Biol. Chem. 270, 19591-19598.
32. Nakayama, C., Teplow, D. B. & Harshey, R. M. (1987). Structural domains in phage Mu transposase: identification of the site-specific DNA-binding domain. Proc. Natl Acad. Sci. USA, 84, 1809-1813.
33. Polard, P. & Chandler, M. (1995). Bacterial transposases and retroviral integrases. Mol. Microbiol. 15, 13-23.
34. Priess, H., Brauer, B., Schmidt, C. & Kamp, D. (1987). Sequence of the left end of Mu. In Phage Mu (Symonds, N., Toussaint, A., Putte, P. V. D. & Howe, M. M., eds), pp. 277-296, Cold Spring Harbor Laboratory, NY.
35. Rice, P., Craigie, R. & Davies, D. R. (1996). Retroviral integrases and their cousins. Curr. Opin. Struct. Biol. 6, 76-83.
36. Riordan, J. F. & Vallee, B. L. (1967). Methods in Enzymology, 11, 541-548.
37. Saraste, M., Sibbald, P. R. & Wittinghofer, A. (1990). The P-loop - a common motif in ATP- and GTP-binding proteins. Trends Biochem. Sci. 15, 430-434.
38. Surette, M. G. & Chaconas, G. (1991). Stimulation of the Mu DNA strand cleavage and intramolecular strand transfer reactions by the Mu B protein is independent of stable binding of the Mu B protein to DNA. J. Biol. Chem. 266, 17306-17313.
39. Surette, M. G., Buch, S. J. & Chaconas, G. (1987). Transpososomes: stable protein-DNA complexes involved in the in vitro transposition of bacteriophage Mu DNA. Cell, 49, 253-262.
40. Surette, M. G., Harkness, T. & Chaconas, G. (1991). Stimulation of the Mu A protein-mediated strand cleavage reaction by the Mu B protein, and the requirement of DNA nicking for stable type 1 transpososome formation. In vitro transposition characteristics of mini-Mu plasmids carrying terminal base pair mutations. J. Biol. Chem. 266, 3118-3124.
41. Teplow, D. B., Nakayama, C., Leung, P. C. & Harshey, R. M. (1988). Structure-function relationships in the transposition protein B of bacteriophage Mu. J. Biol. Chem. 263, 10851-10857.
42. Watson, M. A. & Chaconas, G. (1996). Three-site synapsis during Mu DNA transposition: a critical intermediate preceding engagement of the active site. Cell, 85, 435-445.
43. Wu, Z. & Chaconas, G. (1994). Characterization of a region in phage Mu transposase that is involved in interaction with the Mu B protein. J. Biol. Chem. 269, 28829-28833.
44. Wu, Z. & Chaconas, G. (1995). A novel DNA binding and nuclease activity in domain III of Mu transposase: evidence for a catalytic region involved in donor cleavage. EMBO J. 14, 3835-3843.
45. Wu, Z. & Chaconas, G. (1997). The Mu transposase tetramer is inactive in unassisted strand transfer: an auto-allosteric effect of Mu A promotes the reaction in the absence of Mu B. J. Mol. Biol. 267, 132-141.