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Volumn 279, Issue 5349, 1998, Pages 407-409

An Arabidopsis MADS box gene that controls nutrient-induced changes in root architecture

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; NONHUMAN; PLANT GROWTH; PLANT ROOT; PLASTICITY; PRIORITY JOURNAL; TRANSGENIC PLANT;

EID: 0032536001     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.279.5349.407     Document Type: Article
Times cited : (1043)

References (35)
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    • note
    • The ANR1 cDNA sequence is deposited in GenBank (accession number Z97057).
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    • Arabidopsis thaliana ecotype C24 was transformed with Agrobacterium tumefaciens strains carrying sense and antisense ANR1 constructs. The sense construct was generated by fusing the pANR1 cDNA in the sense orientation to a duplicated cauliflower mosaic virus 35S promoter [F. Guerineau, A. Lucy, P. Mullineaux, Plant Mol. Biol. 18, 815 (1992)]. For the antisense construct, a polymerase chain reaction fragment corresponding to bases 262 to 969 of the pANR1 sequence (which excludes the conserved MADS-box domain) was fused in the antisense orientation to the same promoter. Both constructs were transferred to pBIN19 and introduced into A. tumefaciens by electroporation. Root explants were transformed as described [D. Valvekens, M. Van Montagu, M. Van Lijsebettens, Proc. Natl. Acad. Sci. U.S.A. 85, 5536 (1988)].
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    • --treated C24, the numbers were 3.7 ± 1.1 (top) and 4.4 ± 0.8 (middle).
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    • - response is not mediated by ANR1 (10).
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    • note
    • We thank N. Crawford for the nia1 nia2 mutant, D. Marks for the β-tubulin cDNA, A. Williams for assistance with some experiments, and D. Clarkson for critical reading of the manuscript. IACR-Rothamsted is grant-aided by the Biotechnology and Biological Sciences Research Council.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.