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The ANR1 cDNA sequence is deposited in GenBank (accession number Z97057).
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18
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Arabidopsis thaliana ecotype C24 was transformed with Agrobacterium tumefaciens strains carrying sense and antisense ANR1 constructs. The sense construct was generated by fusing the pANR1 cDNA in the sense orientation to a duplicated cauliflower mosaic virus 35S promoter [F. Guerineau, A. Lucy, P. Mullineaux, Plant Mol. Biol. 18, 815 (1992)]. For the antisense construct, a polymerase chain reaction fragment corresponding to bases 262 to 969 of the pANR1 sequence (which excludes the conserved MADS-box domain) was fused in the antisense orientation to the same promoter. Both constructs were transferred to pBIN19 and introduced into A. tumefaciens by electroporation. Root explants were transformed as described [D. Valvekens, M. Van Montagu, M. Van Lijsebettens, Proc. Natl. Acad. Sci. U.S.A. 85, 5536 (1988)].
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Arabidopsis thaliana ecotype C24 was transformed with Agrobacterium tumefaciens strains carrying sense and antisense ANR1 constructs. The sense construct was generated by fusing the pANR1 cDNA in the sense orientation to a duplicated cauliflower mosaic virus 35S promoter [F. Guerineau, A. Lucy, P. Mullineaux, Plant Mol. Biol. 18, 815 (1992)]. For the antisense construct, a polymerase chain reaction fragment corresponding to bases 262 to 969 of the pANR1 sequence (which excludes the conserved MADS-box domain) was fused in the antisense orientation to the same promoter. Both constructs were transferred to pBIN19 and introduced into A. tumefaciens by electroporation. Root explants were transformed as described [D. Valvekens, M. Van Montagu, M. Van Lijsebettens, Proc. Natl. Acad. Sci. U.S.A. 85, 5536 (1988)].
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3).
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3 by spreading 50 μl of a 100 mM solution over its surface and leaving it overnight to diffuse.
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26
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15444342611
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note
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--treated C24, the numbers were 3.7 ± 1.1 (top) and 4.4 ± 0.8 (middle).
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- response is not mediated by ANR1 (10).
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- itself as well as organic N compounds [W.-R. Scheible et al., Plant J. 11, 671 (1997)].
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note
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We thank N. Crawford for the nia1 nia2 mutant, D. Marks for the β-tubulin cDNA, A. Williams for assistance with some experiments, and D. Clarkson for critical reading of the manuscript. IACR-Rothamsted is grant-aided by the Biotechnology and Biological Sciences Research Council.
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