The 2.15 Å crystal structure of a triple mutant plastocyanin from the cyanobacterium Synechocystis sp. PCC 6803

Journal of Molecular Biology

Volumn 275, Issue 2, 1998, Pages 327-336

  Romero, Antonio ^a   De La Cerda, Berta ^b   Varela, Paloma F ^a   Navarro, José A ^b   Hervás, Manuel ^b   De La Rosa, Miguel A ^b  

^a CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

^b UNIVERSITY OF SEVILLE   (Spain)

Author keywords

Cupredoxins; Electron transfer protein; Plastocyanin; Synechocystis; X ray structure

Indexed keywords

BACTERIAL PROTEIN; LYSINE; PLASTOCYANIN; PROLINE; TYROSINE; RECOMBINANT PROTEIN;

ANABAENA; ARTICLE; CHEMICAL BOND; CRYSTAL STRUCTURE; CYANOBACTERIUM; ELECTRON TRANSPORT; GREEN ALGA; HYDROPHOBICITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; PROTEIN STRUCTURE; R FACTOR; AMINO ACID SEQUENCE; ANIMAL; CHEMICAL STRUCTURE; CHEMISTRY; CHLAMYDOMONAS REINHARDTII; COMPARATIVE STUDY; ELECTRICITY; KINETICS; METABOLISM; MOLECULAR GENETICS; POINT MUTATION; PROTEIN CONFORMATION; PROTEIN SECONDARY STRUCTURE; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; TREE; X RAY CRYSTALLOGRAPHY;

ALGAE; ANABAENA; ANABAENA VARIABILIS; BACTERIA (MICROORGANISMS); CHLOROPHYTA; CYANOBACTERIA; EMBRYOPHYTA; EUKARYOTA; POPULUS BEROLINENSIS; SYNECHOCYSTIS; SYNECHOCYSTIS SP.; SYNECHOCYSTIS SP. PCC 6803;

AMINO ACID SEQUENCE; ANIMALS; CHLAMYDOMONAS REINHARDTII; CRYSTALLOGRAPHY, X-RAY; CYANOBACTERIA; ELECTROSTATICS; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PLASTOCYANIN; POINT MUTATION; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; TREES;

EID: 0032535854     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1997.1455     Document Type: Article

References (44)

1. Andrew, C. R. & Sanders-Loehr, I. (1996). Copper-sulfur proteins: using Raman spectroscopy to predict coordination geometry. Acc. Chem. Res. 29, 365-372.
2. Badsberg, U., Jorgensen, A. M. M., Gesmar, H., Led, J. L., Hammerstad, J. M., Jespersen, L.-L. & Ulstrup, J. (1996). Solution structure of reduced plastocyanin from the blue-green alga Anabaena variabilis. Biochemistry, 35, 7021-7031.
3. Bagby, S., Driscoll, P. C., Harvey, T. S. & Hill, A. O. (1994). High-resolution solution structure of reduced parsley plastocyanin. Biochemistry, 33, 6611-6622.
4. Baker, E. N. (1988). Structure of azurin from Alcaligenes denitrificans. Refinement at 1.8 Å resolution and comparison of the two crystallographically independent molecules. J. Mol. Biol. 203, 1071-1095.
5. Brünger, A., Kuryian, K. & Karplus, M. (1987). Crystallographic R-factor refinement by molecular dynamics. Science, 235, 458-460.
6. CCP4 (1979). The SERC (UK) Collaborative Computing Project No. 4. Daresbury Laboratory, Warrington, UK.
7. Collyer, C. A., Guss, J. M., Sugimura, Y., Yoshizaki, F. & Freeman, H. C. (1990). Crystal structure of plastocyanin from a green alga, Enteromorpha prolifera. J. Mol. Biol. 211, 617-632.
8. Colman, P. M., Freeman, H. C., Guss, J. M., Murata, M., Norris, V. A., Ramshaw, A. M. & Venkatappa, M. P. (1978). X-ray crystal structure analysis of plastocyanin at 2.7 Å resolution. Nature, 272, 319-324.
9. Connolly, M. J. (1983). Analytical molecular surface calculation. J. Appl. Crystallog. 16, 548-558.
10. 6 from Synechocystis 6803. FEBS Letters, 347, 173-177.
11. Durley, R., Chen, L., Lim, L. W., Mathews, F. S. & Davidson, V. L. (1993). Crystal structure analysis of amicyanin and apoamicyanin from Paracoccus denitrificans at 2.0 Å and 1.8 Å resolution. Protein Sci. 2, 739-752.
12. Eftekhar, F. & Schiller, N. L. (1994). Partial purification and characterization of a mannulona-specific alginate lyase from Pseudomonas aeruginosa. Curr. Microbiol. 29, 37-42.
13. Engh, R. A. & Huber, R. (1991). Accurate bond and angle parameters for x-ray protein structure refinement. Acta Crystallog. sect. A, 47, 392-400.
14. Giebel, L. B. & Spritz, R. A. (1990). Site-directed mutagenesis using a double-stranded DNA fragment as a PCR primer. Nucl. Acids Res. 18, 4947.
15. Gross, E. L. (1996). In Oxygenic Photosynthesis: The Light Reactions: (Ort, D. R. & Yocum, C. F., eds), pp. 413-429, Kluwer, The Netherlands.
16. Guss, J. M. & Freeman, H. C. (1983). Structure of oxidized plastocyanin at 1.6 Å resolution. J. Mol. Biol. 169, 521-563.
17. Guss, J. M., Harrowell, P. R., Murata, M., Norris, V. A. & Freeman, H. C. (1986). Crystal structure analyses of reduced (CuI) poplar plastocyanin at six pH values. J. Mol. Biol. 192, 361-387.
18. Guss, J. M., Bartunik, H. D. & Freeman, H. C. (1992). Accuracy and precision in protein structure analysis. Restrained least-squares refinement of the structure of poplar plastocyanin at 1.33 Å resolution. Acta Crystallog. sect. B, 48, 790-811.
19. Hervás, M., Navarro, F., Navarro, J. A., Chávez, S., Díaz, A., Florencio, F. J. & De la Rosa, M. A. (1993). Synechocystis 6803 plastocyanin isolated from both the cyanobacterium and E. coli transformed cells are identical. FEBS Letters, 319, 257-260.
20. 6 and plastocyanin oxidation by photosystem I. Biochim. Biophys. Acta, 1184, 235-241.
21. 6 to photosystem I. Experimental evidence on the evolution of the reaction mechanism. Biochemistry, 34, 11321-11326.
22. 6 in Anabaena PCC 7119 Synechocystis PCC 6803 and spinach. Biochemistry, 35, 2693-2698.
23. Hippler, M., Reichert, J., Sutter, M., Zak, E., Altschmied, L., Schroer, U., Hermann, R. G. & Haehnel, W. (1996). The plastocyanin binding domain of photosystem I. EMBO J. 15, 6374-6384.
24. Hoshino, T. & Kageyama, M. (1980). Purification and properties of a binding protein for branched-chain amino acids in Pseudomonas aeruginosa. J. Bacteriol. 141, 1055-1063.
25. Jones, T. A., Zou, J.-Y., Cowan, S. W. & Kjeldgaard, M. (1991). Improved methods for building protein models in electron density maps and the location of errors in these models. Acta Crystallog. sect. A, 47, 110-119.
26. Kannt, A., Young, S. & Bendall, D. S. (1996). The role of acidic residues of plastocyanin in its interaction with cytochrome f. Biochim. Biophys. Acta, 1277, 115-126.
27. Kraulis, P. J. (1991). MOLSCRIPT: a program to produce both detailed and schematic plots of protein structures. J. Appl. Crystallog. 24, 946-950.
28. Laskowski, R. A., MacArthur, M. W., Moss, D. S. & Thornton, J. M. (1993). PROCHECK: a program to check the stereochemical quality of protein structures. J. Appl. Crystallog. 26, 283-291.
29. Leslie, A. G. W. (1991). In Crystallographic Computing. vol. 5, pp. 50-61, Oxford University Press, Oxford.
30. Luzzati, V. (1952). Traitement statistyique des erreurs dans la détermination des structures cristallines. Acta Crystallog. sect. A, 5, 802-810.
31. Moore, J. M., Case, D. A., Chazin, W. J., Gippert, G. P., Havel, T. F., Dowls, R. & Wright, P. E. (1988). Three-dimensional solution structure of plastocyanin from the green alga Scenedesmus obliquus. Science, 240, 314-317.
32. Moore, J. M., Lepre, C. A., Gippert, G. P., Chazin, W. J., Case, D. A. & Wright, P. E. (1991). High-resolution solution structure of reduced french bean plastocyanin and comparison with the crystal structure of poplar plastocyanin. J. Mol. Biol. 221, 533-555.
33. 6f to photosystem I. J. Biol. Inorg. Chem. 2, 11-22.
34. Navaza, J. (1994). AMoRe: an automated package for molecular replacement. Acta Crystallog. sect. A, 50, 157-163.
35. Nicholls, A., Bharadwaj, R. & Honig, B. (1993). GRASP-graphical representation and analysis of surface properties. Biophys. J. 64, A166.
36. Ramachandran, G. N. & Sasisekharan, V. (1968). Conformation of polypeptides and proteins. Adv. Protein Chem. 23, 283-437.
37. Redinbo, M. R., Cascio, D., Choukair, M. K., Rice, D., Merchant, S. & Yeates, T. O. (1993). The 1.5 Å crystal structure of plastocyanin from the green alga Chlamydomonas reinhardtii. Biochemistry, 32, 10560-10567.
38. Redinbo, M. R., Yeates, T. O. & Merchant, S. (1994). Plastocyanin: structural and functional analysis. J. Bioenerg. Biomembr. 26, 49-66.
39. Rögner, M., Nixon, P. J. & Dinner, B. A. (1990). Purification and characterization of photosystem I and photosystem II core complexes from wild-type and phycocyanin-deficient strains of the cyanobacterium Synechocystis PCC 6803. J. Biol. Chem. 265, 6189-6196.
40. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
41. Sigfridsson, K., Young, S. & Hansson, O. (1996). Structural dynamics in the plastocyanin-photosystem 1 electron-transfer complex as revealed by mutant studies. Biochemistry, 35, 1249-1257.
42. Sykes, A. G. (1991). Plastocyanin and the blue copper proteins. Struct. Bond. 75, 175-224.
43. Turk, D. (1992). Weiterentwicklung eines Programms für Molekülgraphik und Elektronendichte-Manipulation und seine Anwendung auf verschiedene Protein-Strukturaufklärungen, PhD thesis, Technische Universität, München.
44. 551 and nitrite reductase. Eur. J. Biochem. 194, 109-118.