A spectrophotometric assay for allicin and alliinase (Alliin lyase) activity: Reaction of 2-nitro-5-thiobenzoate with thiosulfinates

Analytical Biochemistry

Volumn 265, Issue 2, 1998, Pages 317-325

  Miron, Talia ^a   Rabinkov, Aharon ^a   Mirelman, David ^a   Weiner, Lev ^a   Wilchek, Meir ^a  

^a WEIZMANN INSTITUTE OF SCIENCE   (Israel)

Author keywords

2 nitro 5 thiobenzoate; 5,5' dithio bis (2 nitrobenzoic acid); Allicin; Alliinase; Garlic extract

Indexed keywords

COVALENT BONDS; SPECTROPHOTOMETERS; SPECTROPHOTOMETRY; SULFUR COMPOUNDS;

2-NITRO-5-THIOBENZOATE; ALLICIN; ALLIINASE; GARLIC EXTRACT; NITROBENZOIC ACIDS;

ENZYME ACTIVITY;

ALLICIN; ALLIIN; BENZOIC ACID ESTER DERIVATIVE; GARLIC EXTRACT; LYASE; THIOSULFINIC ACID DERIVATIVE;

ANALYTIC METHOD; ARTICLE; DISULFIDE BOND; ENZYME ACTIVITY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; SPECTROPHOTOMETRY;

ALLIUM SATIVUM;

EID: 0032534797     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1998.2924     Document Type: Article

References (26)

1. Koch H. P., Lawson L. D. Garlic: The Science and Therapeutic Application of Allium sativum l. and Related Species. 1996;Williams & Wilkins, Baltimore.
2. Block E. Angew. Chem. 31:1992;1135-1178.
3. Lawson L. D. Phytomedicines of Europe: Their Chemistry and Biological Activity. 1998;Am. Chem. Soc. Washington. p. 176-209.
4. Han J., Lawson L., Han G., Han P. Anal. Biochem. 225:1995;157-160.
5. Stoll A., Seebeck E. Adv. Enzymol. 11:1951;377-400.
6. Cavallito C. J., Bailey J. H. J. Am. Chem. Soc. 66:1944;1950-1951.
7. Wills E. D. Biochem. J. 63:1956;514-520.
8. Prasad K., Laxdal V. A., Yu M., Raney B. L. Mol. Cell. Biochem. 154:1996;55-63.
9. Rabinkov A., Miron T., Konstantinovski L., Wilchek M., Mirelman D., Weiner L. Biochim. Biophys. Acta. 1379:1998;233-244.
10. Jansen H., Müller B., Knobloch K. Planta Med. 53:1987;559-562.
11. Müller B. Dtsch. Apoth. Ztg. 129:1989;2500-2504.
12. Koch J., Berger L., Vieregge-Reiter C. Planta Med. 55:1989;327-331.
13. Lawson L. D., Wang Z. J., Hughes B. G. Planta Med. 57:1991;363-370.
14. Block E., Naganathan S., Putnam D., Zhao S. H. J. Agric. Food Chem. 40:1992;2418-2430.
15. Degani Y., Patchornik A. J. Org. Chem. 36:1971;2727-2728.
16. Ellman G. L. Arch. Biochem. Biophys. 82:1959;70-77.
17. Riddles P. W., Blakeley R. L., Zerner B. Anal. Biochem. 94:1979;75-81.
18. Rabinkov A., Wilchek M., Mirelman D. Glycoconj. J. 12:1995;690-698.
19. Schwimmer S., Mazelis M. Arch. Biochem. Biophys. 100:1963;66-73.
20. Rabinkov A., Zhu X. Z., Grafi G., Galili G., Mirelman D. App. Biochem. Biotechnol. 48:1994;149-171.
21. Kazaryan R. A., Kocherginskaya S. A., Goryachenkova E. V. Bioorg. Chem. 5:1979;1257-1264.
22. Gergel D., Cederbaum A. I. Arch. Biochim. Biophys. 347:1997;282-288.
23. Ching T. L., de Jong J., Bast A. Anal. Biochem. 218:1994;377-381.
24. Takata T., Endo T. Patai S. The Chemistry of Sulphinic Acids, Esters and Their Derivatives. 1990;527-575 Wiley, New York.
25. Schoberl A., Grafje H. Ann. Chemie. 617:1958;71-88.
26. Block E., O'Connor J. J. Am. Chem. Soc. 96:1974;3929-3944.