Human matrix metalloproteinase-9: Activation by limited trypsin treatment and generation of monoclonal antibodies specific for the activated form

European Journal of Biochemistry

Volumn 258, Issue 1, 1998, Pages 37-43

  Duncan, Margaret E ^a   Richardson, Jonathan P ^a   Murray, Graeme I ^a   Melvin, William T ^a   Fothergill, John E ^a  

^a UNIVERSITY OF ABERDEEN   (United Kingdom)

Author keywords

Gelatinase B; Matrix metalloproteinase 9; Monoclonal antibodies; Oesophageal cancer; Proenzyme activation

Indexed keywords

GELATINASE B; TRYPSIN;

ANTIBODY SPECIFICITY; ARTICLE; DIAGNOSTIC VALUE; ENZYME ACTIVATION; ESOPHAGUS CANCER; HUMAN; HUMAN TISSUE; PRIORITY JOURNAL;

EID: 0032533729     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2580037.x     Document Type: Article

References (47)

1. Woessner. J. F. (1991) Matrix metalloproteinases and their inhibitors in connective tissue remodelling, FASEB. J. 5, 2145 -2154.
2. Murphy, G. & Docherty, A. J. P. (1992) The matrix metalloproteinases and their inhibitors, Am. J. Respir. Cell Mol. Biol. 7, 120 - 125.
3. Stetler-Stevenson, W. G., Liotta, L. A. & Kleiner, D. E. (1993) Extracellular matrix 6: role of matrix metalloproteinases in tumour invasion and metastasis, FASEB. J. 7, 1434-1441.
4. Chambers, A. F. & Matrisian, L. M. (1997) Changing views of the role of matrix metalloproteinases in metastasis. J. Natl Cancer Inst. 89, 1260-1270.
5. Becker, J. W., Marcy, A. I., Rokosz, L. L., Axel, M. G., Burbaum, J. J., Fitzgerald, P. M. D., Cameron, P. M., Esser, C. K., Hagmann, W. K., Hermes. J. D. & Springer. J. P. (1995) Stromelysin-1 : three-dimensional structure of the inhibited catalytic domain and of the C-truncated proenzyme, Protein Struct. 4, 1966-1976.
6. Lovejoy, B., Cleasby, A., Hassell. A. M., Longley, K., Luther, M. A., Weigl, D., McGeehan, G., McElroy, A. B., Drewry, D., Lambert. M. H. & Jordan. S. R. (1994) Structure of the catalytic domain of fibroblast collagenase complexed with an inhibitor. Science 263, 375-377.
7. Li, J., Brick, P., O'Hare, M. C., Skarzynski, T., Lloyd, L. F., Curry, V. A., Clark. I. M., Bigg, H. F., Hazleman, B. L., Cawston, T. E. & Blow, D. M. (1995) Structure of full-length porcine synovial collagenase reveals a C-terminal domain containing a calcium-linked, tour-bladed β-propeller, Structure 3, 541-549.
8. Browner, M. F., Smith, W. W. & Castelhano, A. L. (1995) Matrilysin-inhibitor complexes: common themes among metallopro-teinases. Biochemistry 34, 6602-6610.
9. Bode, W., Reinemer, P., Huber, R., Kleine, T., Schnierer, S. & Tschesche, H. (1994) The X-ray crystal structure of the catalytic domain of human neutrophil collagenase inhibited by a substrate analogue reveals the essentials for catalysis and specificity, EMBO J. 13, 1263-1269.
10. Libson, A. M., Gittis. A. G., Collier, I. E., Marmer, B. L., Goldberg, G. I. & Lattman, E. E. (1995) Crystal structure of the haemopexin-like C-terminal domain of gelatinase A, Nature Struct. Biol. 2, 938-942.
11. Gohlke. U., Gomis-Rüth, F. X., Crabbe, T., Murphy, G., Docherty, A. J. P. & Bode, W. (1996) The C-terminal (haemopexin-like) domain structure of human gelatinase A (MMP2): structural implications for its function, FEBS Lett. 378, 126-130.
12. Gomis-Rüth, F X., Goehlke, U., Betz, M., Knäuper. V., Murphy, G., López-Otín, C. & Bode, W. (1996) The helping hand of collagenase-3 (MMP-13): 2.7 Å crystal structure of its C-terminal haemopexin-like domain, J. Mol. Biol. 264, 556-566.
13. Docherty. A. J. P., Lyons, A., Smith. B. J., Wright, E. M., Stephens, P. E., Harris, T. J. R., Murphy, G. & Reynolds, J. J. (1985) Sequence of human tissue inhibitor of metalloproteinases and its identity to erythroid-potentiating activity. Nature 318, 66-69.
14. Stetler-Stevenson, W. G., Krutzsch, H. C., Liotta, L. A. (1989) Tissue inhibitor of metalloproteinase (TIMP-2) A new member of the metalloproteinase inhibitor family, J. Biol. Chem. 264. 17374 - 17378.
15. Uría, J. A., Ferrando. A. A., Velasco, G., Freije, J. M. P. & LópezOtin. C. (1994) Structure and expression in breast tumors of human TIMP-3, a new member of the metalloproteinase inhibitor family, Cancer Res. 54, 2091-2094.
16. Greene. J., Wang. M. S., Liu, Y. L. E., Raymond, L. A., Rosen, C. & Shi. Y. N. E. ( 1996) Molecular cloning and characterisation of human tissue inhibitor of metalloproteinase 4, J. Biol. Chem. 271, 30 375-30 380.
17. Wilhelm, S. M., Collier, I. E., Marmer, B. L., Eisen. A. Z., Grant, G. A. & Goldberg, G. I. (1989) SV40-transformed human lung fibroblasts secrete a 92-kDa type-IV collagenase which is identical to that secreted by normal human macrophages. J. Biol. Cheat. 264, 17213-17221.
18. Mackay, A. R., Hartzler. J. L., Pelina, M. D. & Thorgiersson. U. P. (1990) Studies on the ability of 65-kDa tumor-cell gelatinases to degrade type-IV collagen, J. Biol. Chem. 265, 21929-21934.
19. ⌈ 92000 gelatinase secreted by normal and malignant human cells -isolation and characterisation of the enzyme from HT1080 tumor cells. Cancer Res. 50. 6162-6170.
20. Murphy, G., Ward, R., Hembry, R. M., Reynolds. J. J., Khün, K. & Tryggvason, K. (1989) Characterisation of gelatinase from pig polymorphonuclear leukocytes - a metalloproteinase resembling tumor type IV collagenase, Biochem. J. 258, 463-472.
21. Tryggvason, K., Höyhtyä, M. & Pyke, C. (1993) Type IV collagenases in invasive tumors. Breast Cancer Res. Treat. 24, 209 -218.
22. Okada. Y., Gonoji, Y., Naka. K., Tomita. K., Nakanishi, I., Iwata, K., Yamashita, K. & Hayakawa, T. (1992) Matrix metalloproteinase 9 (92-kDa gelatinase/type IV collagenase) from HT 1080 human fibrosarcoma cells. J. Biol. Chem. 267, 21712-21719.
23. 1 92000 gelatinase release correlates with the metastatic phenotype in transformed rat embryo cells. Cancer Res. 50, 3872-3877.
24. Ballin, M., Gomez, D. E., Sinha, C. C. & Thorgiersson, U. P. {1988} Ras oncogene mediated induction of a 92 kDa metalloproteinase -strong correlation with the malignant phenotype. Biochem. Hiophys. Res. Commun. 154, 832-838.
25. Yamagata, S., Tanaka, R., Ito. Y. & Shimizu, S. (1989) Gelatinases of murine metastatic tumor cells. Biochem. Biophys. Res. Comm. 158, 228-234.
26. Bernhard, E. J., Gruber, S. B. & Muschel, R. J. (1994) Direct evidence linking expression of matrix metalloproteinase 9 (92 kDa gelatinase/collagenase) to the metastatic phenotype in transformed rat embryo cells. Proc. Natl Acad. Sci. USA 91, 4293-4297.
27. Murray. G. I., Duncan, M. E., O'Neil. P., McKay, J., Melvin, W T. & Fothergill, J. E. ( 1998) Matrix-metalloproteinase-1 is associated with poor prognosis in oesophageal cancer, J. Pathol. 185, 256-261.
28. Stearns, M. & Stearns, M. E. (1996) Evidence for increased activated metalloproteinase 2 (MMP-2) expression associated with human prostate cancer progression, Oncol. Res. 8, 69-75.
29. Reinemer, P., Grams, F., Huber, R., Kleine, T., Schnierer, S., Piper, M., Tschesche, H. & Bode, W. (1994) Structural implications for the role of the N terminus in the 'superactivation' of collagenases. A crystallographic study, FEBS Lett. 338, 227-233.
30. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
31. Matsudaira, P. ( 1987) Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes, J. Biol. Chem. 262, 10035-10038.
32. Duncan, M. E., McAleese, S. M., Booth, N. A., Melvin, W. T. & Fothergill, J. E. (1992) A simple enzyme-linked immunosorbent assay (ELlSA) for the neuron-specific γ isozyme of human enolase (NSE) using monoclonal antibodies raised against synthetic peptides corresponding to isozyme sequence differences, J. lmmunoL Methods 151, 227-236.
33. Dunbar, B. & Wilson, S. B. (1994) A buffer exchange procedure giving enhanced resolution to polyacrylamide gels prerun for protein sequencing, Anal. Biochem. 216, 227-229.
34. Stetler-Stevenson, W. G., Krutzsch, H. C., Wacher, M. P., Margulies, I. M. K. & Liotta, L. A. (1989) The activation of human type IV collagenase proenzyme. Sequence identification of the major conversion product following organomercurial activation, J. Biol. Chem. 264, 1353-1356.
35. Hiraga, A., Kemp, B. E. & Cohen, P. (1987) Further studies on the structure of the glycogen-bound form of protein phosphatase-1 from rabbit skeletal muscle, Eur. J. Biochem. 163, 253-258.
36. Barnes, T. S., Shaw, P. M., Burke, M. D. & Melvin, W. T. (1987) Monoclonal antibodies against human cytochrome P-450 recognising different pregnenolone 16α-carbonitrile-inducible rat cytochrome P-450, Biochem. J. 248. 301-304.
37. Murray, G. I., Duncan, M. E., Melvin, W. T. & Fothergill, J. E. (1993) Immunohistochemistry of neurone specific enolase with γ subunit specific anti-peptide monoclonal antibodies. J. Clin. Pathol. 46, 993-996.
38. McKay, J. A., Weaver, R. J., Murray, G. I., Ewen, S. W. B., Melvin, W. T. & Burke, M. D. (1995) Expression of microsomal epoxide hydrolase in normal and neoplastic kidney. J. Histochem. Cytochem. 43, 615-620.
39. Morodomi, T., Ogata, Y., Sasaguri, Y., Morimatsu, M. & Nagase. H. (1992) Purification and characterisation of matrix metalloproteinase 9 from U937 monocytic leukaemia and HT1080 fibrosarcoma cells, Biochem. J. 285, 603-611.
40. Murphy, G., Docherty, A. J. P., Hembry, R. M. & Reynolds, J. J. (1991) Metalloproteinases and tissue damage, Br. J. Rheumatol. 30 (Suppl. 1), 25-31.
41. Sang, Q. A. & Douglas, D. A. (1996) Computational sequence analysis of matrix metalloproteinases. J. Prot. Chem. 15, 137 - 160.
42. Ogata, Y., Enghild, J. J. & Nagase, H. (1992) Matrix metalloproteinase 3 (stromelysin) activates the precursor for the human matrix metalloproteinase 9, J. Biol. Chem. 267, 3581-3584.
43. Shapiro, S. D., Fliszar, C. J., Broekelmann. T. J., Mecham, R. P., Senior, R. M. & Welgus, H. G. (1995) Activation of the 92-kDa gelatinase by stromelysin and 4-aminophenylmercuric acetate, J. Biol. Chem. 270, 6351-6356.
44. Ries, C., Lottspeich, F., Dittman, K. H. & Petrides, P. E. (1996) HL-60 leukaemia cells produce an autocatalytically truncated form of matrix metalloproteinase-9 with impaired sensitivity to inhibition by tissue inhibitors of metalloproteinases. Leukaemia 10. 1520-1526.
45. Goldberg, G. I., Strongin, A., Collier, I. E., Genrich. L. T. & Warmer, B. L. (1992) Interaction of 92-kDa type IV collagenase with the tissue inhibitor of metalloproteinases prevents dimerisation, complex formation with interstitial collagenase, and activation of the proenzyme with stromelysin, J. Biol. Chem. 267. 4583-4591.
46. O'Connell, J. P., Willenbrock, F., Docherty, A. J. P., Eaton. D. & Murphy, G. (1994) Analysis of the role of the COOH-terminal domain in the activation, proteolytic activity, and tissue inhibitor of metalloproteinase interactions of gelatinase B. J. Biol. Chem. 269. 14967-14973.
47. Murphy. G. J. P., Murphy, G. & Reynolds, J. J. (1991) The origin of matrix metalloproteinases and their familial relationships, FEBS Lett. 289, 4-7.