UDP-galactose 4-epimerase from Kluyveromyces fragilis: Reconstitution of holoenzyme structure after dissociation with parachloromercuribenzoate

European Journal of Biochemistry

Volumn 257, Issue 2, 1998, Pages 427-433

  Majumdar, Siddhartha ^a   Bhattacharjee, Hiranmoy ^a   Bhattacharyya, Debasish ^a   Bhaduri, Amarnath ^a  

^a INDIAN INSTITUTE OF CHEMICAL BIOLOGY   (India)

Author keywords

Holoenzyme; Kluyveromyces fragilis; P chloromercuribenzoate; Subunit dissociation; UDP galactose 4 epimerase

Indexed keywords

4 CHLOROMERCURIBENZOIC ACID; 8 ANILINO 1 NAPHTHALENESULFONIC ACID; APOENZYME; DIMER; DITHIOTHREITOL; FUNGAL PROTEIN; NICOTINAMIDE ADENINE DINUCLEOTIDE; URIDINE DIPHOSPHATE GALACTOSE; URIDINE DIPHOSPHATE GLUCOSE 4 EPIMERASE;

ARTICLE; CONTROLLED STUDY; DISSOCIATION; ENZYME RECONSTITUTION; ENZYME STRUCTURE; IONIC STRENGTH; KLUYVEROMYCES; NONHUMAN; PRIORITY JOURNAL;

KLUYVEROMYCES; KLUYVEROMYCES MARXIANUS;

EID: 0032532538     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2570427.x     Document Type: Article

References (25)

1. Gabrial, O., Kalckar, H. M. & Darrow, R. A. (1975) UDP-galactose 4-epimerase, in Subunit enzymes: biochemistry and function (Ebner, K. B., ed.) pp. 85-134, Marcel-Dekker, Inc., New York.
2. Gabrial, O. & Lanten, L. V. (1978) The interconversion of monosaccharides, Int. Rev. Biochem. 16, 1-36.
3. Frey, P. A. (1987) Complex pyridine nucleotide dependent transformations, in Pyridine nucleotide coenzymes: chemical, biochemical and medical aspects (Dolphin, D., Poulson, R. & Avarmovie, O., eds) vol. 2B, pp. 462-447, Wiley, New York.
4. Bhattacharjee, H. & Bhaduri, A. (1992) Distinct functional roles of two active site thiols in UDP-galactose 4-epimerase from Kluyveromyces fragilis, J. Biol. Chem. 267, 11714-11720.
5. Ghelis, C. & Yon, J. M. (1982) Folding-unfolding of oligomeric proteins, in Protein folding, pp. 470-500, Academic Press, Orlando, Fl, USA.
6. Jaenicke, R. & Rudolph, R. (1986) Refolding and association of oligomeric proteins, Methods Enzymol. 131, 218-250.
7. Price, N. C. (1993) Assembly of multi-subunit structures, in Mechanisms of protein folding (Pain, R. H., ed.) pp. 160-193, Oxford University Press.
8. Bhattacharyya, D. (1993) Reversible folding of UDP-galactose 4-epimerase from yeast Kluyveromyces fragilis, Biochemistry 32, 9726-9734.
9. Darrow, R. A. & Rodstrom, R. (1970) Uridine diphosphate galactose 4-epimerase from Saccharomyces fragilis: Studies on the relationship between quarternary structure and catalytic activity, J. Biol. Chem. 145, 2036-2042.
10. Darrow, R. A. & Rodstrom, R. (1966) Subunit association and subunit assembly of uridine diphosphate galactose 4-epimerase from yeast, Proc. Natl Acad. Sci. USA 55, 205-212.
11. Ray, M. & Bhaduri, A. (1980) Presence of two conformationally vicinal sulfydryl groups at the active site of UDP-galactose 4-epimerase from Sacharomyces fragilis, J. Biol. Chem. 255, 10777-10781.
12. Darrow, R. A. & Rodstrom, R. (1968) Purification and properties of uridine diphosphate galactose 4-epimerase from yeast, Biochemistry 7, 1645-1654.
13. Regnier, F. E. (1983) High-performance liquid chromatography of proteins, Methods Enzymol. 91, 137-190.
14. Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982) Molecular cloning: a laboratory manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor NY, pp. 466-467.
15. Zalitis, J., Uram, M. Bowser, A. M. & Feingold, D. S. (1972) UDP-glucose dehydrogenase from beef liver, Methods Enzymol. 28, 430-435.
16. Traut, T. W. (1994) Dissociation of enzyme oligomers: A mechanism for allosteric regulation, Crit. Rev. Biochem. Mol. Biol. 29, 125-163.
17. Slavik, J. (1992) Anilinonaphthalene sulfonate as a probe of membrane composition and function, Biochim. Biophys. Acta 694, 1-25.
18. Wong, S. S., Cassim, J. Y. & Frey, P. A. (1978) Escherichia coli uridine diphosphate galactose 4-epimerase: circular dichroism of the protein and protein bound dihydronicotinamide adenine dinucleotide, Biochemistry 17, 516-520.
19. Samanta, A. K. & Bhaduri, A. (1982) Interaction of 8-anilino 1-naphthalene sulphonic acid with UDP-galactose 4-epimerase from Saccharomyces fragilis, Ind. J. Biochem. Biophys. 19, 320-323.
20. Mukherjee, S. & Bhaduri, A. (1986) UDP-galactose 4-epimerase from Saccharomyces fragilis: Presence of an essential arginine residue at the substrate binding site of the enzyme, J. Biol. Chem. 261, 4519-4524.
21. Dutta, S., Maity, N. R. & Bhattacharyya, D. (1997) Reversible folding of UDP-galactose 4-epimerase from Esherichia coli, Eur. J. Biochem. 244, 407-413.
22. Maxell, H. S. (1957) The enzymic interconversion of uridine diphospho-galactose and uridine diphospho-glucose, J. Biol. Chem. 229, 139-151.
23. Ray, M. & Bhaduri, A. (1975) UDP-glucose 4-epimerase from Saccharomyces fragilis. Allosteric kinetics with UDP-glucose as substrate, J. Biol. Chem. 250, 4373-4375.
24. Fischer, G. & Schmid, F. X. (1990) The mechanism of protein folding: implications of in vitro refolding models for de novo protein folding and translocation in the cell, Biochemistry 29, 2205-2212.
25. Garel, J.-R. (1992) Folding of large proteins: Multidomain and multifunctional proteins, in Protein folding (Creighton, T. E., ed.) pp. 405-453, W. H. Freeman and Company, New York.