Axonal injury alters alternative splicing of the retinal NR1 receptor: The preferential expression of the NR1b isoforms is crucial for retinal ganglion cell survival

Journal of Neuroscience

Volumn 18, Issue 20, 1998, Pages 8278-8291

  Kreutz, Michael R ^a,b   Böckers, Tobias M ^b,c   Bockmann, Jürgen ^b,c   Seidenbecher, Constanze I ^a   Kracht, Bettina ^a,b   Vorwerk, Christian K ^a   Weise, Jens ^a   Sabel, Bernhard A ^a  

^a OTTO VON GUERICKE UNIVERSITY   (Germany)

^b LEIBNIZ INSTITUTE FOR NEUROBIOLOGY   (Germany)

^c UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

Alternative splicing; Antisense targeting; In situ hybridization; Lesion; NMDA receptors; NR1; NR2; Optic nerve crush; Retina; RT PCR; Western blots

Indexed keywords

N METHYL DEXTRO ASPARTIC ACID RECEPTOR;

ALTERNATIVE RNA SPLICING; ANIMAL CELL; ARTICLE; CELL SURVIVAL; CONTROLLED STUDY; IN SITU HYBRIDIZATION; MALE; NERVE FIBER; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; RETINA GANGLION CELL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 0032531964     PISSN: 02706474     EISSN: None     Source Type: Journal    
DOI: 10.1523/jneurosci.18-20-08278.1998     Document Type: Article

References (22)

1. Anantharam V, Panchal RG, Wilson A, Koltchine VV, Treistman SN, Bayley H (1992) Combinatorial RNA splicing alters the surface charge on the NMDA receptor. FEBS Lett 305:27-30.
2. Aoki C, Venktasan C, Go CG, Mong JA, Dawson TM (1994) Cellular and subcellular localization of NMDA-R1 subunit immunoreactivity in the visual cortex of adult and neonatal rats. J Neurosci 14:5202-5222.
3. Back T, Hoehn-Berlage M, Kohno K, Hossmann KA (1994) Diffusion nuclear magnetic resonance imaging in experimental stroke. Correlation with cerebral metabolites. Stroke 25:494-500.
4. Bessho Y, Nawa H, Nakanishi S (1994) Selective upregulation of an NMDA receptor subunit mRNA in cultured cereballao granule cells by KCH-induced depolarization and NMDA treatment. Neuron 12: 87-95.
5. Bien A, Humphrey MF, Seidenbecher C, Sabel BA, Kreutz MR (1996) Apoptosis in retinal ganglion cells after a controlled crush of the optic nerve. Soc Neurosci Abstr 126:2.
6. Bien A, Seidenhecher CI, Böckers TM, Sabel BA, Kreutz MR (1998) Apoptotic versus necrotic characteristics of retinal ganglion cell death after partial optic nerve injury. J Neurotrauma, in press.
7. Böckers TM, Zimmer M, Müller A, Bergmann M, Brose N, Kreutz MR (1994) Expression of the NMDA R1 receptor in selected human brain regions. NeuroReport 5:965-969.
8. Brandstätter JH, Hartveit E, Sassoc̀-Pognetto M, Wässle H (1994) Expression of NMDA and high-affinity kainatc receptor subunit mRNAs in the adult rat retina. Eur J Neurosci 6:1100-1112.
9. Bresnick GH (1989) Excitotoxins: a possible new mechanism for the pathogenesis of ischemic retinal damage. Arch Ophtalmol 107:339-341.
10. Choi DW (1992) Excitotoxic cell death. J Neurobiol 23:1261-1276.
11. Condorelli DF, Dell'Albani P, Aronica E, Genazzani AA, Casabona G, Corsaro M, Balazs R, Nicoletti F (1993) Growth conditions differentially regulate the expression of alpha-amino-3-hydroxy-5-methylisoxazole-4-propionate (AMPA) receptor subunits in cultured neurons. J Neurochem 61:2133-2139.
12. Della Vedova F, Bonecchi L, Bianchetti A, Fariello RG, Speciale C (1994) Age-related changes in the relative abundance of NMDAR1 mRNA splice variants in the rat brain. NeuroReport 5:581-584.
13. + after impact trauma in rats. Adv Neurol 52:225-232.
14. Dreycr EB, Pan ZH, Storm S, Lipton SA (1994) Greater sensitivity of larger retinal ganglion cells to NMDA-mediated cell death. NeuroReport 5:629-631.
15. Durand GM, Gregor P, Zheng X, Bennett MVL, Uhl GR, Zukin RS (1992) Cloning of an apparent splice variant of the rat N-methyl-D-aspartate receptor NMDAR1 with altered sensitivity to polyamines and activators of protein kinase C. Proc Natl Acad Sci USA 89:9359-9363.
16. Durand GM, Bennett MVL, Zukin RS (1993) Splice variants of the N-methyl-D-aspartate receptor NR1 identify domains involved in regulation by polyamines and protein kinase C. Proc Natl Acad Sci USA 90:6731-6735.
17. Faden AI, Demediuk P, Panter SS, Vink R (1989) The role of excitatory amino acids and NMDA receptors in traumatic brain injury. Science 244:798-800.
18. Fukuda Y (1977) A three-group classification of retinal ganglion cells: histological and physiological studies. Brain Res 119:327-344.
19. Gennaralli TA, Thibault LE, Tipperman R, Tomei G, Sergot R (1989) Axonal injury in the optic nerve: a model simulating diffuse axonal injury in the brain. J Neurosurg 7:244-253.
20. Silveira LC, Russelakis-Carneiro M, Perry VH (1994) The ganglion cell response to optic nerve injury in the cat: differential responses revealed by neurofibrillar staining. J Neurocytol 23:75-86.
21. Smith DH, Okiyama K, Thomas MJ, McIntosh TK (1993) Effects of the excitatory amino acid receptor antagonists kynurenate and indole-2-carboxylic acid on behavioral and neurochemical outcome following experimental brain injury. J Neurosci 13:5383-5392.
22. Soares DH, Lowenstein DH, Hicks RR, Perlman KG, McIntosh TK (1992) Development of prolonged focal cerebral edema and regional cation changes following experimental brain injury in the rat. J Neurochem 58:1845-1852.