Phosphorylation-specific antibodies for human cardiac troponin-I

European Journal of Biochemistry

Volumn 256, Issue 3, 1998, Pages 535-540

  Al Hillawi, Eman ^a   Chilton, Derrick ^a   Trayer, Ian P ^a   Cummins, Peter ^a  

^a UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

Cardiac muscle; Monoclonal antibody; Phosphorylation; Phosphorylation specific antibodies; Troponin I

Indexed keywords

TROPONIN;

AMINO ACID SEQUENCE; ANTIBODY SPECIFICITY; ARTICLE; CROSS REACTION; DOG; HEART CONTRACTION; HEART MUSCLE; HUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION;

EID: 0032530637     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1998.2560535.x     Document Type: Article

References (16)

1. Ray, K. P. & England, P. J. (1976) Phosphorylation of the inhibitory subunit of troponin and its effect on the calcium dependence of cardiac myofibril adenosine triphosphatase, FEBS Lett. 70, 11-16.
2. Solaro, R. J., Moir, A. J. G. & Perry, S. V. (1976) Phosphorylation of troponin-I and the inotropic effect of adrenaline in perfused rabbit heart, Nature 262, 615-617.
3. Reiffert, S. U., Jaquet, K., Heilmeyer, L. M. G., Ritchie, M. D. & Geeves, M. A. (1996) Bisphosphorylation of cardiac troponin-I modulates the Ca2+-dependent binding of myosin subfragment S1 to reconstituted thin filaments, FEBS Lett. 384, 43-47.
4. 2+ affinity of cardiac troponin-C, J. Biol. Chem. 270, 30 773-30 780.
5. Al-Hillawi, E., Bhandari, D. G., Trayer, H. R. & Trayer, I. P. (1995) The effects of phosphorylation of cardiac troponin-I on its interactions with actin and cardiac troponin-C, Eur. J. Biochem. 228, 962-970.
6. Dohet, C., Al-Hillawi, K., Trayer, I. P. & Rüegg, J. C. (1995) Reconstitution of skinned cardiac fibres with human recombinant cardiac troponin-I mutants and troponin-C, FEBS Lett. 377, 131-134.
7. Dohet, C., Kögler, H., Al-Hillawi, E., Trayer, I. P. & Rüegg, J. C. (1996a) Do mutants imitating the mono- and bis-phosphorylated form of human cardiac troponin-I modulate calcium sensitivity in reconstituted skinned cardiac muscle fibres? Biophys. J. 70, A382.
8. Dohet, C., Kögler, H., Al-Hillawi, H., Trayer, I. P. & Rüegg, J. C. (1996b) Calcium-sensitivity modulation by substituting mutant troponin-I in permeabilized myocardium, J. Mol. Biol. Cell Cardiol. 28, A218.
9. Cummins, B., Auckland, M. L. & Cummins, P. (1987) Cardiac-specific troponin-I radioimmunoassay in the diagnosis of acute myocardial infarction, Amer. Heart J. 113, 1333-1344.
10. Al-Hillawi, E., Minchin, S. D. & Trayer, I. P. (1994) Overexpression of humand cardiac troponin-I and troponin-C in Escherichia coli and their purification and characterisation, Eur. J. Biochem. 225, 1195-1201.
11. Cummins, B. & Cummins, P. (1987) Cardiac specific trononin-1 release in canine experimental myocardial infection: development of a sensitive enzyme-linked immunoassay, J. Mol. Cell. Cardiol. 19, 999-1010.
12. Mejbaum-Katzenellbogen, W. & Dobryszcka, W. M. (1959) New methods for quantitative of serum proteins separated by paper chromatography, Clin. Chim. Acta 4, 515-522.
13. Cummins, B., Russell, G. J. & Cummins, P. (1991) A monoclonal antibody that distinguishes phospho- and dephosphorylated forms of cardiac troponin-I, Biochem. Soc. Trans. 19, 161S.
14. Stahli, C., Miggiano, V., Stocker, J., Staehelin, T., Haring, P. & Takacs, B. (1983) Distinction of epitopes by monoclonal antibodies, Methods Enzymol. 92, 242-253.
15. Jaquet, K., Thieleczek, R. & Heilmeyer, L. M. G. (1995) Pattern formation on cardiac troponin-I by consecutive phosphorylation and dephosphorylation, Eur. J. Biochem. 231, 486-490.
16. Swiderek, K., Jaquet, K., Meyer, H. E. & Heilmeyer, L. M. G. (1988) Cardiac troponin-I isolated from bovine heart, contains two adjacent phosphoserines - a first example of phosphoserine determination by derivatisation to S-ethylcysteine, Eur. J. Biochem. 176, 335-342.