Protein expression by Streptococcus uberis in co-culture with bovine mammary epithelial cells

FEMS Microbiology Letters

Volumn 166, Issue 2, 1998, Pages 237-242

  Fang, Weihuan ^a   Luther, Douglas A ^a   Oliver, Stephen P ^a  

^a UNIVERSITY OF TENNESSEE   (United States)

Author keywords

Bovine mastitis; Mammary epithelial cells; Protein expression; Streptococcus uberis

Indexed keywords

BACTERIA (MICROORGANISMS); BOVINAE; CICONIIFORMES; MYCOBACTERIUM AVIUM COMPLEX (MAC); STREPTOCOCCUS UBERIS;

BACTERIAL ANTIGEN; BACTERIAL PROTEIN; MEMBRANE PROTEIN;

ANIMAL; ANIMAL DISEASE; ARTICLE; BIOSYNTHESIS; CATTLE; CATTLE DISEASE; CELL LINE; COCULTURE; CULTURE MEDIUM; CYTOLOGY; DAIRYING; EPITHELIUM CELL; FEMALE; IMMUNOLOGY; METABOLISM; MICROBIOLOGY; PATHOGENICITY; POLYACRYLAMIDE GEL ELECTROPHORESIS; STREPTOCOCCUS; STREPTOCOCCUS INFECTION; UDDER; WESTERN BLOTTING;

ANIMALS; ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; BLOTTING, WESTERN; CATTLE; CELL LINE; COCULTURE TECHNIQUES; CULTURE MEDIA, SERUM-FREE; DAIRYING; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EPITHELIAL CELLS; FEMALE; MAMMARY GLANDS, ANIMAL; MASTITIS, BOVINE; MEMBRANE PROTEINS; STREPTOCOCCAL INFECTIONS; STREPTOCOCCUS;

EID: 0032529967     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1574-6968.1998.tb13896.x     Document Type: Article

References (13)

1. Almeida, R.A. and Oliver, S.P. (1993) Antiphagocytic effect of the capsule of Streptococcus uberis. J. Vet. Med. B 40, 707-714.
2. Almeida, R.A., Luther, D.A., Kumar, S.K., Calvinho, L.F. and Oliver, S.P. (1996) Adherence of Streptococcus uberis to bovine mammary epithelial cells and extracellular matrix proteins. J. Vet. Med. B 43, 385-392.
3. Doane, R.M., Oliver, S.P., Walker, R.D. and Shull, E.P. (1987) Experimental infection of lactating bovine mammary glands with Streptococcus uberis in quarters colonized with Corynebacterium bovis. Am. J. Vet. Res. 48, 749-754.
4. Finlay, B. B. and Falkow, S. (1997) Common themes in microbial pathogenicity revisited. Microbiol. Mol. Biol. Rev. 61, 136-169.
5. Gilbert, F.B., Luther, D.A. and Oliver, S.P. (1997) Induction of surface-associated proteins of Streptococcus uberis by cultivation of extracellular matrix components and bovine mammary epithelial cells. FEMS Microbiol. Lett. 156, 161-164.
6. Jacobsen, G. (1994) Protein blotting using semi-dry electrophoretic transfer equipment. In: Protein Blotting - A Practical Approach (B.S. Dunbar, Ed.) pp. 53-70. Oxford University Press, Oxford, UK.
7. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277, 680-685.
8. Leigh, J.A. (1993) Activation of bovine plasminogen by Streptococcus uberis. FEMS Microbiol. Lett. 114, 67-71.
9. Matthews, K.R. and Oliver, S.P. (1993) Encapsulation of Streptococci isolated from bovine milk. J. Vet. Med. B 40, 597-602.
10. Matthews, K.R., Almeida, R.A. and Oliver, S.P. (1994) Bovine mammary epithelial cell invasion by Streptococcus uberis. Infect. Immun. 62, 5641-5646.
11. Mekalanos, J.J. (1992) Environmental signals controlling expression of virulence determinants in bacteria. J. Bacteriol. 174, 1-7.
12. Oakley, B.R., Kirsch, D.R. and Morris, N.R. (1980) A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels. Anal. Biochem. 105, 361-363.
13. Pollard, J.W. (1994) Two-dimensional polyacrylamide gel electrophoresis of proteins. In: Methods in Molecular Biology, Vol. 32, Basic Proteins and Peptide Protocols (J.M. Walker, Ed.), pp. 73-84. Humana Press, Totowa, NJ, USA.