Complementation of the Mpg1 mutant phenotype in Magnaporthe grisea reveals functional relationships between fungal hydrophobins

EMBO Journal

Volumn 17, Issue 14, 1998, Pages 3838-3849

  Kershaw, Michael J ^a   Wakley, Gavin ^a   Talbot, Nicholas J ^a  

^a UNIVERSITY OF EXETER   (United Kingdom)

Author keywords

Appressorium development; Conidiation; Fungal pathogenicity; Hydrophobin; Rodlet layer

Indexed keywords

FUNGAL PROTEIN; HYDROPHOBIN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS NIDULANS; CONTROLLED STUDY; DELETION MUTANT; FUNGAL GENETICS; GENE DISRUPTION; MUTANT; NEUROSPORA CRASSA; NONHUMAN; PATHOGENICITY; PHENOTYPE; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; SCHIZOPHYLLUM COMMUNE;

EID: 0032528025     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.14.3838     Document Type: Article

References (40)

1. Asgeirsdottir, S.A., Halsall, J.R. and Casselton, L.A. (1997) Expression of two closely linked hydrophobin genes of Coprinus cinereus is monokaryon-specific and down-regulated by the oid-1 mutation. Fungal Genet. Biol., 22, 54-63.
2. Beckerman, J.L. and Ebbole, D.J. (1996) MPG1, a gene encoding a fungal hydrophobin of Magnaporthe grisea, is involved in surface recognition, Mol. Plant-Microbe Interact., 9, 450-456.
3. Bell-Pederson, D., Dunlap, J.C. and Loros, J.J. (1992) The Neurospora circadian clock-controlled gene, ccg-2, is allelic to eas and encodes a fungal hydrophobin required for formation of the conidial rodlet layer. Genes Dev., 6, 2382-2394.
4. Chiu, W., Niwa,Y., Zeng, W., Hirano, T., Kobayashi, H. and Sheen, J. (1996) Engineered GFP as a vital reporter in plants. Carr. Biol., 6, 325-330.
5. Crawford, M.S., Chumley, F.G., Weaver, C.G. and Valent, B. (1986) Characterization of the heterokaryotic and vegetative diploid phases of Magnaporthe grisea. Genetics, 114, 1111-1129.
6. de Jong, J.C. McCormack, B.J., Smirnoff, N. and Talbot, N.J. (1997) Glycerol generates turgor in rice blast. Nature, 389, 244-245.
7. de Vocht, M.L. et al. (1998) Structural characterization of the hydrophobin SC3, as a monomer and after self-assembly at hydrophobic/hydrophilic interfaces. Biophys. J., 74, 2059-2068.
8. Feinberg, A.P. and Vogelstein, B. (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem., 132, 6-13.
9. Hamer, J.E., Howard, R.J., Chumley, F.G. and Valent, B. (1988) A mechanism for surface attachment in spores of a plant pathogenic fungus. Science, 239, 288-290.
10. Howard, R.J. and Valent, B. (1996) Breaking and entering: host penetration by the fungal rice blast pathogen Magnaporthe grisea. Annu. Rev. Microbiol., 50, 491-512.
11. Lau, G. and Hamer, J.E. (1996) Regulatory genes controlling MPG1 expression and pathogenicity in the rice blast fungus Magnaporthe grisea. The Plant Cell, 8, 771-781.
12. Lauter, F.R., Russo, V.E.A. and Yanofsky, C. (1992) Developmental and light regulation of Eas, the structural gene for the rodlet protein of Neurospora crassa. Genes Dev., 6, 2373-2381.
13. Lee, Y.H. and Dean, R.A. (1993) cAMP regulates infection structure formation in the plant pathogenic fungus Magnaporthe grisea. The Plant Cell, 5, 693-700.
14. Notteghem, J.L. and Silue, D. (1992) Distribution of the mating type alleles in Magnaporthe grisea populations pathogenic on rice. Phytopathology, 82, 421-424.
15. Ou, S.H. (1985) Rice Diseases. Commonwealth Mycological Institute, C.A.B., Kew, Surrey, UK, pp. 109-201.
16. Prasher, D.C., Eckenrode, V.K., Ward, W.W., Prendergast, F.G. and Cormier, M.J. (1992) Primary structure of the Aequoria victoria green-fluorescent protein. Gene, 111, 229-233.
17. Punt, P.J., Oliver, R.P., Dingemanse, M.A., Powels, P.H. and van den Hondel, C.A.M.J.J. (1987) Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene, 56, 117-124.
18. Sambrook, J., Fritsch, E.F. and Maniatis T (1989) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
19. Schuren, F.H.J. and Wessels, J.G.H. (1990) Two genes specifically expressed in fruiting dikaryons of Schizophyllum commune: homologies with a gene not regulated by mating type genes. Gene, 90, 199-205.
20. Sokal, R.R. and Rohlf, F.J. (1981) Biometry. W.H. Freeman and Co., San Francisco, CA, USA.
21. Spanu, P. (1997) HCF-1, a hydrophobin from the tomato pathogen Cladosporium fulvum. Gene, 193, 89-96.
22. St. Leger, R.J., Staples, R.C. and Roberts, D.W. (1992) Cloning and regulatory analysis of starvation-stress gene, ssgA, encoding a hydrophobin-like protein from the entomopathogenic fungus Metarhizium anisopliae. Gene, 120, 119-124.
23. Stearns, T. (1995) Green fluorescent protein-the green revolution. Curr. Biol., 5, 262-264.
24. Stringer, M.A. and Timberlake, W.E. (1995) dewA encodes a fungal hydrophobin component of the Aspergillus spore wall. Mol. Microbiol., 16, 33-44.
25. Stringer, M.A., Dean, R.A., Sewell, T.C. and Timberlake, W.E. (1991) Rodletless, a new Aspergillus developmental mutant induced by directed gene inactivation. Genes Dev., 5, 1161-1171.
26. Sweigard, J.A., Chumley, F.G., Carroll, A., Farrall, L and Valent, B. (1997) A series of vectors for fungal transformation. Fungal Genet. Newslett., 44, 52-53.
27. Talbot, N.J. (1995) Having a blast: exploring the pathogenicity of Magnaporthe grisea. Trends Microbiol., 3, 9-16.
28. Talbot, N.J. (1997) Fungal biology: growing into the air. Curr. Biol., 7, R78-R82.
29. Talbot, N.J., Ebbole, D.J. and Hamer, J.E. (1993) Identification and characterization of MPG1, a gene involved in pathogenicity from the rice blast fungus Magnaporthe grisea. The Plant Cell, 5, 1575-1590.
30. Talbot, N.J., Kershaw, M.J., Wakley, G.E., de Vries, O.M.H., Wessels, J.G.H. and Hamer, J.E. (1996) MPG1 encodes a fungal hydrophobin involved in surface interactions during infection-related development by Magnaporthe grisea. The Plant Cell, 8, 985-999.
31. Templeton, M.D., Rikkerink, E.H.A. and Beever, R.E. (1994) Small, cysteine-rich proteins and recognition in fungal-plant interactions. Mol. Plant-Microbe Interact., 7, 320-325.
32. Templeton, M.D., Greenwood, D.R. and Beever, R.E. (1995) Solubilization of Neurospora crassa rodlet proteins and identitication of the predominant protein as the proteolytically processed eas (ccg-2) gene product. Exp. Mycol., 19, 166-169.
33. Timberlake, W.E. (1980) Developmental gene regulation in Aspergillus, nidulans. Dev. Biol., 78, 497-510.
34. Van Wetter, M.-A., Schuren, F.H.J., Schuurs, T.A. and Wessels, J.G.H. (1996) Targeted mutation of the SC3 hydrophobin gene of Schizophyllum commune affects formation of aerial hyphae. FEMS Microbiol. Lett., 140, 265-269.
35. Wessels, J.G.H. (1997) Hydrophobins: proteins that change the nature of the fungal surface. Adv. Microbial Physiol., 38, 1-45.
36. Wessels, J.G.H., de Vries, O.M.H., Ásgeirsdóttir, S.A. and Schuren, F.H.J. (1991) Hydrophobin genes involved in formation of aerial hyphae and fruit bodies in Schizophyllum. The Plant Cell, 3, 793-799.
37. Wösten, H.A.B., de Vries, O.M.H. and Wessels, J.G.H. (1993) Interfacial self-assembly of a fungal hydrophobin into a hydrophobic rodlet layer. The Plant Cell, 5, 1567-1574.
38. Wösten, H.A.B., Ásgeirsdóttir, S.A., Krook, J.H., Drenth, J.H.H. and Wessels, J.G.H. (1994a) The fungal hydrophobin SC3p self-assembles at the surface of aerial hyphae as a protein membrane constituting the hydrophobic rodlet layer. Eur. J. Cell Biol., 63, 122-129.
39. Wösten, H.A.B., Schuren, F.H.J. and Wessels, J.G.H. (1994b) Interfacial self-assembly of a hydrophobin into an amphipathic membrane mediates fungal attachment to hydrophobic surfaces. EMBO J., 13, 5848-5854.
40. Wösten, H.A.B., Ruardy, T.G., Van der Mei, H.C., Busscher, H.J. and Wessels, J.G.H. (1995) Interfacial self-assembly of a Schizophyllum commune hydrophobin into an insoluble amphipathic membrane depends on surface hydrophobicity. Colloids and Surfaces B, 5, 189-195.