A Salmonella typhimurium mutant unable to utilize fatty acids and citrate is avirulent and immunogenic in mice

FEMS Microbiology Letters

Volumn 163, Issue 2, 1998, Pages 129-134

  Utley, Maryjane ^a   Franklin, David P ^a   Krogfelt, Karen A ^b   Laux, David C ^a   Cohen, Paul S ^a  

^a Washburn Hall Rm #300 Univ R   (United States)

^b STATENS SERUM INSTITUT   (Denmark)

Author keywords

Avirulent; Fatty acid citrate mutant; Immunogenic; S. typhimurium

Indexed keywords

CITRIC ACID; FATTY ACID;

ANIMAL CELL; ARTICLE; BACTERIAL VIRULENCE; BACTERIUM MUTANT; FATTY ACID OXIDATION; GENE DELETION; GENE INSERTION; IMMUNOGENICITY; MOUSE; MUTAGENICITY; NONHUMAN; PRIORITY JOURNAL; RECEPTOR GENE; SALMONELLA TYPHIMURIUM; TRANSPOSON;

ANIMALS; BACTERIAL VACCINES; CITRIC ACID; DNA TRANSPOSABLE ELEMENTS; FATTY ACIDS; FECES; LIVER; MICE; MICE, INBRED BALB C; MUTAGENESIS, INSERTIONAL; SALMONELLA INFECTIONS, ANIMAL; SALMONELLA TYPHIMURIUM; SPLEEN; VACCINATION; VIRULENCE;

ANIMALIA; BACTERIA (MICROORGANISMS); NEGIBACTERIA; SALMONELLA TYPHIMURIUM; TYPHIMURIUM;

EID: 0032526683     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(98)00162-1     Document Type: Article

References (17)

1. Curtiss, R. III and Kelly, S.M. (1987) Salmonella typhimurium deletion mutants lacking adenylate cyclase and cyclic AMP receptor protein are avirulent and immunogenic. Infect. Immun. 55, 3035-3043.
2. Tacket, C.O., Hone, D.M., Curtiss, R. III, Kelly, S.M., Losonsky, G., Guers, L., Harris, A.M., Edelman, R. and Levine, M.M. (1992) Comparison of the safety and immunogenicity of ΔaroC ΔaroD and Δcya Δcrp Salmonella typhi strains in adult volunteers. Infect. Immun. 60, 536-541.
3. Jones, P.W., Dougan, G., Hayward, C., Mackensie, N., Collins, P. and Chatfield, S.N. (1991) Oral vaccination of calves against experimental salmonellosis using a double aro mutant of Salmonella typhimurium. Vaccine 9, 29-34.
4. Stocker, B.A.D., Hoiseth, S.K. and Smith, B.P. (1983) Aromatic-dependent Salmonella sp. as live vaccine in mice and calves. Dev. Biol. Stand. 53, 47-54.
5. Miller, S.I., Loomis, W.P., Alpuche-Aranda, C., Behlau, I. and Hohmann, E. (1993) The PhoP virulence regulon and live oral Salmonella vaccines. Vaccine 11, 122-125.
6. Hohmann, E.L., Oletta, C.A., Killeen, K.P. and Miller, S.I. (1996) PhoP/phoQ deleted-Salmonella typhi (Ty800) is a safe and immugenic single-dose typhoid fever vaccine in volunteers. J. Infect. Dis. 173, 1408-1414.
7. Elliot, T. and Roth, J.R. (1988) Characterization of Tn10d-Cam: A transposition- defective Tn10 specifying chloramphenicol resistance. Mol. Gen. Genet. 213, 332-338.
8. Smith, H.O. and Levine, M. (1967) A phage P22 gene controlling integration of prophage. Virology 31, 207-216.
9. Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual, p. 68. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
10. Brenner, D.J. (1984) Facultatively anaerobic Gram-negative rods. In: Bergey's Manual of Systematic Bacteriology, Vol. 1 (Krieg, N.R., Ed.), pp. 408-516. Williams and Wilkins, Baltimore, MD.
11. Gulig, P.A. and Curtiss, R. III (1987) Plasmid-associated virulence of Salmonella typhimurium. Infect. Immun. 55, 2891-2901.
12. Pascopella, L., Raupach, B., Ghori, N., Monack, D., Falkow, S. and Small, P.L.C. (1995) Host restriction phenotypes of Salmonella typhi and Salmonella gallinarum. Infect. Immun. 63, 4329-4335.
13. Jones, B.D., Ghori, N. and Falkow, S. (1995) Salmonella typhimurium initiates murine infection by penetrating and destroying the specialized M cells of the Peyer's patches. J. Exp. Med. 180, 15-23.
14. McFarland, W.C. and Stocker, B.A.D. (1987) Effect of different purine auxotrophic mutations on mouse-virulence of a Vi-positive strain of Salmonella dublin and of two strains of Salmonella typhimurium. Microb. Pathog. 3, 129-141.
15. Nimmo, H.G. (1987) The tricarboxylic acid cycle and anaplerotic reactions. In: Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology (Ingraham, J.L. et al., Eds.), pp. 156-169. American Society for Microbiology, Washington, DC.
16. Krivan, H.C., Franklin, D.P., Wang, W., Laux, D.C. and Cohen, P.S. (1992) Phosphatidylserine found in intestinal mucus serves as a sole source of carbon and nitrogen for salmonellae and Esherichia coli. Infect. Immun. 60, 3943-3946.
17. Mahan, M.J., Tobias, J.W., Slauch, J.M., Hanna, P.C., Collier, J.R. and Mekalanos, J.J. (1995) Antibiotic-based selection for bacterial genes that are specifically induced during infection of a host. Proc. Natl. Acad. Sci. USA 92, 669-673.