Calcium as a regulator of Bacillus megaterium cytoplasmic proteolytic activity in vitro

FEMS Microbiology Letters

Volumn 162, Issue 2, 1998, Pages 283-288

  Váchová, Libuše ^a   Kučerová, Helena ^a  

^a INSTITUTE OF MICROBIOLOGY   (Czech Republic)

Author keywords

Calcium bionding compound; Enzyme processing and activation; Intracellular serine protease; Protease inhibitor

Indexed keywords

CALCIUM; CONGOCIDINE;

ARTICLE; BACILLUS MEGATERIUM; CULTURE MEDIUM; CYTOPLASM; ENZYME ACTIVITY; IN VITRO STUDY; PRIORITY JOURNAL; PROTEIN DEGRADATION;

EID: 0032525266     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(98)00134-7     Document Type: Article

References (17)

1. Strongin, A.Y., Izotova, L.S., Abramov, Z.T., Gorodetsky, D.I., Ermakova, L.M., Baratova, L.A., Belyanova, L.P. and Stepanov, V.M. (1978) Intracellular serine protease of Bacillus subtilis: sequence homology with extracellular subtilisins. J. Bacteriol. 133, 1401-1411.
2. Srivastava, O.P. and Aronson, A.I. (1981) Isolation and characterization of a unique protease from sporulating cells of Bacillus subtilis. Arch. Microbiol. 129, 227-232.
3. Koide, Y., Nakamura, A., Uozumi, T. and Beppu, T. (1986) Cloning and sequencing of the major intracellular serine protease gene of Bacillus subtilis. J. Bacteriol. 167, 110-116.
4. Sheehan, S.M. and Switzer, R.L. (1990) Intracellular serine protease 1 of Bacillus subtilis is formed in vivo as an unprocessed, active protease in stationary cells. J. Bacteriol. 172, 473-476.
5. 2+ concentrations. Biochem. Mol. Biol. Int. 40, 947-954.
6. Burnett, T.J., Shankweiler, G.W. and Hageman, J.H. (1986) Activation of intracellular serine proteinase in Bacillus subtilis cells during sporulation. J. Bacteriol. 165, 139-145.
7. 2+-dependent serine proteinase in growing and nongrowing Bacillus megaterium. Folia Microbiol. 42, 299-302.
8. Nishino, T. and Murao, S. (1986) Interaction of proteinaceous protease inhibitor of Bacillus subtilis with intracellular proteases from the same strain. Agric. Biol. Chem. 50, 3065-3070.
9. Millet, J. and Gregoire, J. (1979) Characterization of an inhibitor of the intracellular protease from Bacillus subtilis. Biochimie 61, 385-391.
10. Chaloupka, J., Severin, A.I., Sastry, K.J., Kučerová, H. and Strnadová, M. (1982) Differences in the regulation of exocellular proteinase synthesis during growth and sporogenesis of Bacillus megaterium. Can. J. Microbiol. 28, 1214-1218.
11. Kučerová, H., Moravcová, J., Váchová, L., Ludvík, J. and Chaloupka, J. (1993) Netropsin suppresses the rise of activity of an intracellular proteolytic system in sporulating Bacillus megaterium. Curr. Microbiol. 26, 287-292.
12. 2+ concentration. Folia Microbiol. 38, 201-204.
13. Váchová, L. and Moravcová, J. (1993) Two microassays for determination of a wide range of proteolytic activities using Azocoll as substrate. Biochem. Mol. Biol. Int. 30, 311-318.
14. Sedlák, M., Vinter, V., Adamec, J., Vohradský, J., Voburka, Z. and Chaloupka, J. (1993) Heat shock applied early in sporulation affects heat resistance of Bacillus megaterium spores. J. Bacteriol. 175, 8049-8052.
15. Shiga, Y., Yamagata, H. and Udaka, S. (1993) Characterization of the gene encoding an intracellular proteinase inhibitor of Bacillus subtilis and its role in regulation of the major intracellular proteinase. J. Bacteriol. 175, 7130-7137.
16. Chavira, R., Burnett, T.J. and Hageman, J.H. (1984) Assaying proteinases with Azocoll. Anal. Biochem. 136, 446-450.
17. Bronner, F., Nash, W.C. and Golub, E.E. (1975) Calcium transport in Bacillus megaterium. In: Spores VI (Gerhardt, P., Costilow, R.N. and Sadoff, H.L., Eds.), pp. 356-361. Am. Soc. Microbiol., Washington, DC.