Determination of deoxyribonucleoside triphosphate pool sizes in ribonucleotide reductase cDNA transfected human KB cells

Biochemical Pharmacology

Volumn 55, Issue 10, 1998, Pages 1657-1665

  Zhou, Bing Sen ^a   Ker, Rhonda ^a   Ho, Roger ^a   Yu, Jonathan ^a   Zhao, Yong Ren ^a   Shih, Jennifer ^a   Yen, Yun ^a  

^a CITY OF HOPE NATIONAL MEDICAL CENTER   (United States)

Author keywords

Deoxyribonucleoside triphosphate pools; Drug resistance; Hydroxyurea; Ribonucleotide reductase

Indexed keywords

DEOXYRIBONUCLEOSIDE TRIPHOSPHATE; HYDROXYUREA; RIBONUCLEOTIDE REDUCTASE;

ARTICLE; CANCER CELL CULTURE; CELL STRAIN KB; DNA SYNTHESIS INHIBITION; DNA TRANSFECTION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME KINETICS; ENZYME SPECIFICITY; GENE OVEREXPRESSION; HUMAN; HUMAN CELL; NORTHERN BLOTTING; PRIORITY JOURNAL; PROTEIN EXPRESSION;

CLONE CELLS; DEOXYRIBONUCLEOTIDES; DNA, COMPLEMENTARY; DRUG RESISTANCE, NEOPLASM; HUMANS; HYDROXYUREA; RIBONUCLEOTIDE REDUCTASES; RNA, MESSENGER; TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 0032524942     PISSN: 00062952     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-2952(98)00042-2     Document Type: Article

References (25)

1. Bianchi V, Pontis E, Reichard P. Changes of deoxyribonucleoside triphosphate pools induced by hydroxyurea and their relation to DNA synthesis. J Biol Chem. 261:1986;16037-16042.
2. Kunz BA. Genetic effects of deoxyribonucleotide pool imbalances. Environ Mutagen. 4:1982;695-725.
3. Lewis WH, Wright JA. Ribonucleotide reductase from wild type and hydroxyurea-resistant Chinese hamster ovary cells. J Cell Physiol. 97:1978;87-98.
4. Cory JG, Sato A. Regulation of ribonucleotide reductase activity in mammalian cells. Mol Cell Biochem. 53:1983;257-266.
5. Thelander L, Berg P. Isolation and characterization of expressible cDNA clones encoding the M1 and M2 subunits of mouse ribonucleotide reductase. Mol Cell Biol. 6:1986;3433-3442.
6. Matthews CK, Ji J. DNA precursor asymmetries. Replicational fidelity, and variable genome evolution. Bioessays. 14:1992;295-301.
7. Chang CH, Cheng YC. Substrate specificity of human ribonucleotide reductase from Molt-4F cells. Cancer Res. 39:1979;5081-5086.
8. Thelander M, Graslund A, Thelander L. Subunit M2 of mammalian ribonucleotide reductase. J Biol Chem. 260:1985;2737-2741.
9. Tagger AY, Wright JA. Molecular and cellular characterization of drug resistant hamster cell lines with alterations in ribonucleotide reductase. Int J Cancer. 42:1988;760-766.
10. Albert DA, Nodzenski E, Yim G, Kowalski J. Effect of cyclic AMP on the cell cycle regulation of ribonucleotide reductase M2 subunit messenger RNA concentration of wild-type and mutant S49 T lymphoma cells. J Cell Physiol. 143:1990;251-256.
11. Engstrom Y, Eriksson A, Jildevik I, Skog S, Thelander L, Tribukail B. Cell cycle-dependent expression of mammalian ribonucleotide reductase. Differential regulation of the two subunits. J Biol Chem. 260:1985;9114-9116.
12. Björklund S, Skog S, Tribukait B, Thelander L. S-Phase-specific expression of mammalian ribonucleotide reductase R1 and R2 subunit mRNAs. Biochemistry. 29:1990;5452-5458.
13. Yen Y, Grill SP, Dutschman GE, Chang CN, Zhou BS, Cheng YC. Characterization of a hydroxyurea-resistant human KB cell line with supersensitivity to 6-thioguanine. Cancer Res. 54:1994;3686-3691.
14. Zhou BS, Hsu NY, Pan BC, Doroshow JH, Yen Y. Overexpression of ribonucleotide reductase in transfected human KB cells increases their resistance to hydroxyurea M2 but not M1 is sufficient to increase resistance to hydroxyurea in transfected cells . Cancer Res. 55:1995;1328-1333.
15. Sherman PA, Fyfe JA. Enzymatic assay for deoxyribonucleoside triphosphates using synthetic oligonucleotides as template primers. Anal Biochem. 180:1989;222-226.
16. Steeper JP, Steuart CD. A rapid assay for CDP reductase activity in mammalian cell extracts. Anal Biochem. 34:1970;123-130.
17. Hurta RAR, Wright JA. Mammalian drug resistant mutants with multiple gene amplification Genes encoding the M1 component of ribonucleotide reductase, the M2 component of ribonucleotide reductase, ornithine decarboxylase, p5-8, the H-subunit of ferritin and the L-subunit of ferritin . Biochim Biophys Acta. 1087:1990;165-172.
18. Gao WY, Cara A, Gallo RC, Lori F. Low levels of deoxynucleotides in peripheral blood lymphocytes A strategy to inhibit human immunodeficiency virus type 1 replication . Proc Natl Acad Sci USA. 90:1993;8925-8928.
19. Slabaugh MB, Howell ML, Wang Y, Mathews CK. Deoxyadenosine reverses hydroxyurea inhibition of vaccinia virus growth. J Virol. 65:1991;2290-2298.
20. McClarty GA, Chan AKM, Wright JA. Hydroxyurea-induced conversion of mammalian ribonucleotide reductase to a form hypersensitive to bleomycin. Cancer Res. 12:1986;121-131.
21. Eriksson S, Skog S, Tribukait B, Wallstrom B. Deoxyribonucleoside triphosphate metabolism and the mammalian cell cycle Effects of hydroxyurea on mutant and wild-type mouse S49 T-lymphoma cells . Exp Cell Res. 168:1987;79-88.
22. Stubbe J. Ribonucleotide reductases Amazing and confusing . J Biol Chem. 255:1980;7426-7432.
23. Reichard P, Ehrenberg A. Ribonucleotide reductase - a radical enzyme. Science. 221:1983;514-519.
24. Eriksson S, Thelander L, Akerman M. Allosteric regulation of calf thymus ribonucleoside diphosphate reductase. Biochemistry. 18:1979;2948-2952.
25. Thelander L, Reichard P. Reduction of ribonucleotides. Annu Rev Biochem. 48:1979;133-158.