Tumorigenic conversion of p53-deficient colon epithelial cells by an activated Ki-ras gene

Journal of Clinical Investigation

Volumn 101, Issue 8, 1998, Pages 1572-1580

  Sevignani, Cinzia ^a,b   Wlodarski, Pawel ^a,c   Kirillova, Julie ^a   Mercer, W Edward ^a   Danielson, Keith G ^a   Iozzo, Renato V ^a   Calabretta, Bruno ^a,b  

^a THOMAS JEFFERSON UNIVERSITY   (United States)

^b UNIVERSITY OF MODENA AND REGGIO EMILIA   (Italy)

^c MEDICAL UNIVERSITY OF WARSAW   (Poland)

Author keywords

Antioncogene; Cell cultures; Oncogene; Tumorigenesis

Indexed keywords

ANIMAL EXPERIMENT; ANTINEOPLASTIC ACTIVITY; ARTICLE; CARCINOGENESIS; CELL CULTURE; COLON CANCER; COMBINED IMMUNODEFICIENCY; DISEASE ASSOCIATION; FEMALE; GENE ACTIVATION; GENE MUTATION; IMMUNOCOMPETENCE; MALE; MOUSE; NONHUMAN; PRIORITY JOURNAL;

EID: 0032522978     PISSN: 00219738     EISSN: None     Source Type: Journal    
DOI: 10.1172/JCI919     Document Type: Article

References (29)

1. Fearon, E.R., and B. Vogelstein. 1990. A genetic model for colorectal tumorigenesis. Cell. 61:759-767.
2. Kinzler, K.W., and B. Vogelstein. 1996. Lessons from hereditary colorectal cancer. Cell. 87:159-170.
3. Siddiqui, K.M., and D.P. Chopra. 1984. Primary and long-term epithelial cell cultures from fetal normal colonic mucosa. In Vitro. 20:859-868.
4. Danes, B.S. 1985. Long-term-cultured colon epithelial cell lines from individuals with and without colon cancer genotypes. J. Natl. Cancer Inst. 75:261-267.
5. Vidrich, A., R. Ravindranath, K. Farsi, and S. Targan. 1988. A method for the rapid establishment of normal adult mammalian colonic epithelial cell cultures. In Vitro. 24:188-194.
6. Whitehead, R.H., A. Brown, and P.S. Bhathal. 1987. A method for the isolation and culture of human colonic crypts in collagen gels. In Vitro. 23:436-442.
7. Gibson, P.R., E. Van de Pol, L.E. Maxwell, A. Gabriel, and W.F. Doe. 1989. Isolation of colonic crypts that maintain structural and metabolic viability in vitro. Gastroenterology. 96:283-291.
8. Moyer, M.P., and J.B. Aust. 1984. Human colon cells: culture and in vitro transformation. Science. 224:1445-1447.
9. Whitehead, R.H., P.E. Van Eeden, M.D. Noble, P. Ataliotis, and P.S. Jat. 1993. Establishment of conditionally immortalized epithelial cell lines from both colon and small intestines of adult H-2Kb-tsA58 transgenic mice. Proc. Natl. Acad. Sci. USA. 90:587-591.
10. Donehower, L.A., M. Harvey, B.L. Slagle, M.J. McArthur, C.A. Montgomery, J.S. Butel, and A. Bradley. 1992. Mice deficient for p53 are developmentally normal but susceptible to spontaneous tumors. Nature. 356:215-221.
11. Shirasawa, S., M. Furuse, N. Yokayama, and T. Sasazuki. 1993. Altered growth of human colon cancer cell lines disrupted at activated Ki-RAS. Science. 260:85-88.
12. Goyette, M.C., K. Cho, C.L. Fasching, D.B. Levy, K.W. Kinzler, C. Paraskeva, B. Vogelstein, and E.J. Stanbridge. 1992. Progression of colorectal cancer is associated with multiple tumor suppressor gene defects, but inhibition of tumorigenicity is accomplished by correction of any single defect via chromosome transfer. Mol. Cell. Biol. 12:1387-1395.
13. Baker, S.J., S. Markowitz, E.R. Fearon, J.K. Wilson, and B. Vogelstein. 1990. Suppression of human colorectal cell growth by wild-type p53. Science. 243:912-915.
14. McCoy, M.S., C.I. Bargmann, and R.A. Weinberg. 1984. Human colon carcinoma Ki-ras2 oncogene and its corresponding protooncogene. Mol. Cell. Biol. 4:1577-1582.
15. bcr/abl tyrosine kinase. Proc. Natl. Acad. Sci. USA. 93:13137-13142.
16. Miller, A.D., and G.J. Rosman. 1989. Improved retroviral vectors for gene transfer and expression. Biotechniques. 9:980-985.
17. Markowitz, D., S. Goff, and A. Bank. 1988. A safe packaging line for gene transfer: separating viral genes on two different plasmids. J. Virol. 62: 1120-1124.
18. Huttner, K.M., J.A. Barbosa, G.A. Scangos, D.D. Pratcheva, and F.H. Ruddle. 1981. DNA-medialed gene transfer without carrier DNA. J. Cell Biol. 91:153-156.
19. Danielson, K., A. Martinez-Hernandez, J.R. Hassell, and R.V. Iozzo. 1992. Characterization of a basement membrane-producing cell line from the EHS tumor. Matrix. 11:22-35.
20. Iozzo, R.V. 1984. Biosynthesis of heparan sulfate proteoglycan by human colon carcinoma cells and its localization al the cell surface. J. Cell Biol. 99: 403-117.
21. George, D.L., A.F. Scott, S. Trusko, B. Glick, E. Ford, and D.J. Dorney. 1985. Structure and expression of amplified cKi-ras gene sequences in Y1 mouse adrenal tumor cells. EMBO (Eur. Mol. Biol. Organ.) J. 4:1199-1203.
22. Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
23. Fawcett, D.W. 1981. The Cell. W.B. Saunders, Co., Philadelphia, PA.
24. Yahanda, A.M., J.M. Bruner, L.A. Donehower, and R.S. Morrison. 1995. Astrocytes derived from p53-deficient mice provide a multistep in vitro model for development of malignant gliomas. Mol. Cell. Biol. 15:4249-4258.
25. Harvey, M., A.T. Sands, R.S. Weiss, M.E. Hegi, R.W. Wiseman, P. Pantazis, B.C. Giovanella, M.A. Tainsky, A. Bradley, and L.A. Donehower. 1993. In vitro growth characteristics of embryo fibroblasts isolated from p53-deficient mice. Oncogene. 8:2457-2467.
26. Metz, T., A.W. Harris, and J.M. Adams. 1995. Absence of p53 allows direct immortalization of hematopoietic cells by the myc and raf oncogenes. Cell. 82:29-36.
27. Tanaka, N., M. Ishihara, M. Kitagawa, H. Harada, T. Kimura, T. Matsuyama, M.S. Lamphier, S. Aizawa, T.W. Mak, and T. Taniguchi. 1994. Cellular commitment to oncogene-induced transformation of apoptosis is dependent on the transcription factor IRF-1. Cell. 77:829-839.
28. INKα. Cell. 88:593-602.
29. D'Abaco, G.M., R.H. Whitehead, and A.W. Burgess. 1996. Synergy between Apcmin and an activated ras mutation is sufficient to induce colon carcinomas. Mol. Cell. Biol. 16:884-898.