Identification of a membrane-spanning domain of the thiol-activated pore-forming toxin Clostridium perfringens perfringolysin O: An α-helical to β-sheet transition identified by fluorescence spectroscopy

Biochemistry

Volumn 37, Issue 41, 1998, Pages 14563-14574

  Shepard, Laura A ^a   Heuck, Alejandro P ^b   Hamman, Brian D ^b,e   Rossjohn, Jamie ^c   Parker, Michael W ^c   Ryan, Kathleen R ^b,f   Johnson, Arthur E ^b,d,g   Tweten, Rodney K ^a  

^a UNIVERSITY OF OKLAHOMA COLLEGE OF MEDICINE   (United States)

^b TEXAS A M COLLEGE OF MEDICINE   (United States)

^c ST VINCENT'S INSTITUTE OF MEDICAL RESEARCH   (Australia)

^d TEXAS A AND M UNIVERSITY   (United States)

^e Aurora Biosciences Corporation   (United States)

^f STANFORD UNIVERSITY   (United States)

^g TEXAS A AND M HEALTH SCIENCE CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHOLESTEROL; CLOSTRIDIUM PERFRINGENS TOXIN; CLOSTRIDIUM TOXIN; CYSTEINE; CYTOLYSIN; FLUORESCENT DYE; IODIDE; N,N' DIMETHYL N (IODOACETYL) N' (7 NITROBENZ 2 OXA 1,3 DIAZOLYL)ETHYLENEDIAMINE; NITROXIDE; THIOL; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BILAYER MEMBRANE; CHANNEL GATING; CLOSTRIDIUM PERFRINGENS; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; FLUORESCENCE SPECTROSCOPY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN;

AMINO ACID SEQUENCE; BACTERIAL TOXINS; CLOSTRIDIUM PERFRINGENS; FLUORESCENCE POLARIZATION; FLUORESCENT DYES; HEMOLYSIN PROTEINS; HUMANS; LIGHT; MEMBRANE PROTEINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OXADIAZOLES; PEPTIDE FRAGMENTS; PHOSPHOLIPIDS; PROTEIN STRUCTURE, SECONDARY; SCATTERING, RADIATION; SPECTROMETRY, FLUORESCENCE; SPIN LABELS; SULFHYDRYL COMPOUNDS;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM; CLOSTRIDIUM PERFRINGENS;

EID: 0032514655     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi981452f     Document Type: Article

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