Sol-gel-encapsulated alcohol dehydrogenase as a versatile, environmentally stabilized sensor for alcohols and aldehydes

Journal of the American Chemical Society

Volumn 120, Issue 18, 1998, Pages 4366-4371

  Williams, Amy K ^a   Hupp, Joseph T ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALCOHOL DEHYDROGENASE; ALCOHOL DERIVATIVE; ALDEHYDE DERIVATIVE;

ARTICLE; ENZYME CHEMISTRY; ENZYME SUBSTRATE COMPLEX; FLUORESCENCE; MICROENCAPSULATION; MOLECULAR DYNAMICS; REACTION ANALYSIS; SENSOR;

EID: 0032513711     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja973772c     Document Type: Article

References (34)

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14. We note, however, that detection of vapor-phase ethanol has recently been reported by Lan, Dave, Dunn, Valentine, and Zink (Mater. Res. Soc. Symp. Proc. 1995, 371, 267-76) based on catalytic current assessment within a sol-gel-encapsulated ADH/ferricyanide assembly. Other approaches involve immobilization on membranes with subsequent detection either through potentiometric or optical methods. (See, for example: Wangsa, J.; Arnold, M. Anal. Chem. 1988, 60, 1080-1082.).
15. We note, however, that detection of vapor-phase ethanol has recently been reported by Lan, Dave, Dunn, Valentine, and Zink (Mater. Res. Soc. Symp. Proc. 1995, 371, 267-76) based on catalytic current assessment within a sol-gel-encapsulated ADH/ferricyanide assembly. Other approaches involve immobilization on membranes with subsequent detection either through potentiometric or optical methods. (See, for example: Wangsa, J.; Arnold, M. Anal. Chem. 1988, 60, 1080-1082.).
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19. Exposure of the cloudy gels to substrate resulted in no change in fluorescence. We are currently investigating whether the cloudiness is due to aggregation thus indicating an upper limit on achievable enzyme concentration.
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32. The "Breathalyzer" experiment utilizes the vaporization of alcohol from the subject's lungs to the air. Assuming that three alcoholic drinks in an hour results in a blood alcohol level of ca. 0.1%, an approximate comparison of the "Breathalyzer" response (i.e., fluorescence growth rate) to the trend represented in the vapor-phase (head gas) experiment is possible. The combined kinetic responses are reasonably quantitatively self-consistent. The comparison additionally suggests that the lower limit of detection for the enzyme-based ethanol sensor, as currently configured, is ca. 0.02% alcohol present in the corresponding liquid phase.
33. Examples of successful film-based encapsulation include the following: (a) MacCraith, B. D.; McDonagh, C. M.; Keeffe, G. O.; McEvoy, A. K.; Butler, T.; Sheridan, F. R. Sensors Actuators B 1995, 29, 51-57. (b) Dave, B. C.; Soyez, H.; Miller, J. M.; Dunn, B.; Valentine, J. S.; Zink, J. I. Chem. Mater. 1995, 7, 1431-1434.
34. Examples of successful film-based encapsulation include the following: (a) MacCraith, B. D.; McDonagh, C. M.; Keeffe, G. O.; McEvoy, A. K.; Butler, T.; Sheridan, F. R. Sensors Actuators B 1995, 29, 51-57. (b) Dave, B. C.; Soyez, H.; Miller, J. M.; Dunn, B.; Valentine, J. S.; Zink, J. I. Chem. Mater. 1995, 7, 1431-1434.