Structural basis for a Ca2+-Sensing functions of the metabotropic glutamate receptors

Science

Volumn 279, Issue 5357, 1998, Pages 1722-1725

  Kubo, Yoshihiro ^a   Miyashita, Tomoyuki ^a   Murata, Yoshimichi ^a,b  

^a TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE   (Japan)

^b MEIJI PHARMACEUTICAL UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; CALCIUM ION; GLUTAMIC ACID; GUANINE NUCLEOTIDE BINDING PROTEIN; METABOTROPIC RECEPTOR; ACTIN; CALCIUM; CYCLIC AMP; G PROTEIN COUPLED INWARDLY RECTIFYING POTASSIUM CHANNEL; HYBRID PROTEIN; INWARDLY RECTIFYING POTASSIUM CHANNEL; POTASSIUM CHANNEL;

ANIMAL CELL; ARTICLE; BINDING SITE; BRAIN; CELL STRUCTURE; CHO CELL; CONTROLLED STUDY; DOSE RESPONSE; NERVE CELL PLASTICITY; NONHUMAN; PRIORITY JOURNAL; AMINO ACID SEQUENCE; ANIMAL; CELL SIZE; CHEMISTRY; GENETIC TRANSFECTION; GENETICS; HAMSTER; METABOLISM; MOLECULAR GENETICS; OOCYTE; POINT MUTATION; RAT; SECOND MESSENGER; ULTRASTRUCTURE; XENOPUS LAEVIS;

ACTINS; AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; BRAIN; CALCIUM; CELL SIZE; CHO CELLS; CRICETINAE; CYCLIC AMP; G PROTEIN-COUPLED INWARDLY-RECTIFYING POTASSIUM CHANNELS; GLUTAMIC ACID; MOLECULAR SEQUENCE DATA; OOCYTES; POINT MUTATION; POTASSIUM CHANNELS; POTASSIUM CHANNELS, INWARDLY RECTIFYING; RATS; RECEPTORS, METABOTROPIC GLUTAMATE; RECOMBINANT FUSION PROTEINS; SECOND MESSENGER SYSTEMS; TRANSFECTION; XENOPUS LAEVIS;

EID: 0032512921     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.279.5357.1722     Document Type: Article

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27. The transfected cells were identified by staining with polyclonal antibody to mGluR1 (Chemicon) and Cy3-labeled secondary antibody (Jackson ImmunoResearch Labs, West Grove, PA). The morphology of the actin filaments was visualized by staining with fluorescein isothiocyanate (FITC)-labeled phalloidin.
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31. Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L. Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
32. 2 vector, respectively; R. Murrell-Lagnado for comments on the manuscript; and K. Kubokawa, H. Okado, and K. Takahashi for discussion. Supported by research grants from the ministry of Education, Science, Sports and Culture of Japan for Exploratory Research to Y.K. and for scientific research on the priority area of "Channel-Transporter Correlation" to Y.K. Y.K. is also supported by Core of Research for Evolutional Science and Technology of the Japan Science and Technology Corporation.