Studies on cycloheximide-sensitive and cycloheximide-resistant ribosomes in the yeast Candida maltosa

Gene

Volumn 224, Issue 1-2, 1998, Pages 9-15

  Mutoh, E ^a   Ohta, A ^a   Takagi, M ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

Gene disruption; L41 ribosomal protein; Multi step evolutionary event

Indexed keywords

CYCLOHEXIMIDE;

ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; CANDIDA; FUNGAL GENETICS; GENE CONTROL; GENE DISRUPTION; GENE ISOLATION; MOLECULAR EVOLUTION; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS INHIBITION; RIBOSOME;

AMINO ACID SEQUENCE; ANTIBIOTICS, ANTIFUNGAL; BASE SEQUENCE; CANDIDA; CYCLOHEXIMIDE; DNA, RIBOSOMAL; DRUG RESISTANCE, MICROBIAL; GENE EXPRESSION REGULATION, FUNGAL; GENES, FUNGAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN BIOSYNTHESIS; RIBOSOMAL PROTEINS; RIBOSOMES;

CANDIDA MALTOSA;

EID: 0032509453     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(98)00518-6     Document Type: Article

References (15)

1. Dehoux P., Davies J., Cannon M. Natural cycloheximide resistance in yeast - The role of ribosomal protein L41. Eur. J. Biochem. 213:1993;841-848.
2. Del Pozo L., Abarca D., Hoenicka J., Jimenez A. Two different genes from Schwanniomyces occidentalis determine ribosomal resistance to cycloheximide. Eur. J. Biochem. 213:1993;849-857.
3. Hikiji T., Ohkuma M., Takagi M., Yano K. An improved host-vector system for Candida maltosa using a gene isolated from its genome that complements his5 mutation of Saccharomyces cerevisiae. Curr. Genet. 16:1989;261-266.
4. Kawai S., Hikiji S., Murao S., Takagi T., Yano K. Isolation and sequencing of a gene, C-ADE1, and its use for a host-vector system in Candida maltosa with two genetic markers. Agric. Biol. Chem. 55:1991;59-65.
5. Kawai S., Murao S., Mochizuki M., Shibuya I., Yano K., Takagi M. Drastic alteration of cycloheximide sensitivity by substitution of one amino acid in the L41 ribosomal protein of yeasts. J. Bacteriol. 174:1992;254-262.
6. Mutoh E., Mochizuki M., Ohta A., Takagi M. Inducible expression of a gene encoding an L41 ribosomal protein responsible for the cycloheximide-resistant phenotype in the yeast Candida maltosa. J. Bacteriol. 177:1995;5383-5386.
7. Ohkuma M., Hikiji K., Tanimoto T., Schunck W.-H., Muller H.G., Yano K., Takagi M. Evidence that more than one gene encodes n-alkane-inducible cytochrome P-450s in Candida maltosa, revealed by two-step gene disruption. Agric. Biol. Chem. 55:1991;1757-1764.
8. Ohkuma M., Tanimoto T., Yano K., Takagi M. CYP52(cytochrome P450alk) multigene family in Candida maltosa: molecular cloning and nucleotide sequence of the two tandemly arranged genes. DNA Cell Biol. 10:1991;271-282.
9. Ohkuma M., Muraoka S., Hwang C.W., Ohta A., Takagi M. Cloning of C-URA3 gene and construction of a triple auxotroph (his5, ade1, ura3) as a useful host for genetic engineering of Candida maltosa. Curr. Genet. 23:1993;205-210.
10. Ohkuma M., Zimmer T., Iida T., Schunck W.-H., Ohta A., Takagi M. Isozyme function of n-alkane inducible cytochromes P450 in Candida maltosa revealed by sequential gene disruption. J. Biol. Chem. 273:1998;3948-3953.
11. Schmitt M.E., Brown T.A., Trumpower B.L. A rapid and simple method for preparation of RNA from Saccharomyces cerevisiae. Nucleic Acids Res. 18:1990;3091-3092.
12. Takagi M., Kawai K., Takata Y., Tanaka N., Sunairi M., Miyazaki M., Yano K. Induction of cycloheximide resistance in Candida maltosa by modifying the ribosomes. J. Gen. Appl. Microbiol. 31:1985;267-275.
13. Takagi M., Kawai S., Chang M.C., Shibuya I., Yano K. Construction of a host-vector system of Candida maltosa using an ARS site isolated from its genome. J. Bacteriol. 167:1986;551-555.
14. Takagi M., Kawai S., Shibuya I., Miyazaki M., Yano K. Cloning in Saccharomyces cerevisiae of a cycloheximide resistance gene from the Candida maltosa genome which modifies ribosomes. J. Bacteriol. 168:1986;417-419.
15. Warner J.R. Synthesis of ribosomes in Saccharomyces cerevisiae. Microbiol. Rev. 53:1989;256-271.