Intrastriatal and intraventricular injections of oligodeoxynucleotides in the rat brain: Tissue penetration, intracellular distribution and c-fos antisense effects

Molecular Brain Research

Volumn 63, Issue 1, 1998, Pages 35-52

  Grzanna, Reinhard ^a   Dubin, Joan R ^a   Dent, Gersham W ^a   Ji, Zhongqi ^a   Zhang, Wei ^a   Ho, Siew Peng ^a   Hartig, Paul R ^a  

^a DUPONT COMPANY   (United States)

Author keywords

Antisense oligonucleotide; c fos induction; Confocal microscopy; Intracerebral injection; Phosphorothioate

Indexed keywords

OLIGODEOXYNUCLEOTIDE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRAIN VENTRICLE; DRUG PENETRATION; GENE EXPRESSION REGULATION; GENE INDUCTION; INTRACEREBRAL DRUG ADMINISTRATION; INTRACEREBROVENTRICULAR DRUG ADMINISTRATION; MALE; NONHUMAN; ONCOGENE C FOS; PRIORITY JOURNAL; RAT;

ANIMALS; ANTISENSE ELEMENTS (GENETICS); BIOTIN; BRAIN CHEMISTRY; CORPUS STRIATUM; FLUORESCENT ANTIBODY TECHNIQUE; GENE EXPRESSION; GENE TRANSFER TECHNIQUES; INJECTIONS, INTRAVENTRICULAR; MALE; MICROSCOPY, CONFOCAL; NEURONS; OLIGODEOXYRIBONUCLEOTIDES; PROTO-ONCOGENE PROTEINS C-FOS; RATS; RATS, SPRAGUE-DAWLEY; TIME FACTORS;

ANIMALIA;

EID: 0032506607     PISSN: 0169328X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0169-328X(98)00238-1     Document Type: Article

References (35)

1. Agrawal S., Temsamani J., Tang J.Y. Pharmacokinetics, biodistribution, and stability of oligodeoxynucleotide phosphorothiates in mice. Proc. Natl. Acad. Sci. U.S.A. 88:1991;7595-7599.
2. Beltinger C., Saragovi H.U., Smith R.M., LeSauteur L., Shah N., DeDionisio L., Christensen L., Raible A., Jarett L., Gewirtz A.M. Binding, uptake, and intracellular trafficking of phosphorothioate-modified oligodeoxynucleotides. J. Clin. Invest. 95:1995;1814-1823.
3. Brysch W., Schlingensiepen K.-H. Design and application of antisense oligonucleotides in cell culture, in vivo and as therapeutic agents. Cell. Mol. Neurobiol. 14:1994;557-568.
4. Chiasson B.J., Hooper M.L., Murphy P.R., Robertson H.A. Antisense oligonucleotide eliminates in vivo expression of c-fos in mammalian brain. Eur. J. Pharmacol. 227:1992;453-454.
5. Chiasson B.J., Armstrong J.N., Hooper M.L., Murphy P.R., Robertson H.A. The application of antisense oligonucleotide technology to the brain: some pitfalls. Cell. Mol. Neurobiol. 14:1994;507-521.
6. B.J. Chiasson, M. Hong, M.L. Hooper, J.N. Armstrong, P.R. Murphy, H.A. Robertson, Antisense therapeutics in the central nervous system - the induction of c-fos, in: S. Agrawal (Ed.), Antisense Therapeutics, Humana Press, Totowa, 1996, pp. 225-245.
7. Chiu T.H., Dun S.L., Tang H., Dun N.J. c-fos antisense attenuates Fos expression in rat central neurons induced by hemorrhage. NeuroReport. 5:1994;2178-2180.
8. Chin D.J., Green G.A., Zon F.C., Szoka F.C. Jr., Straubinger R.M. Rapid nuclear accumulation of injected oligodeoxyribonucleotides. The New Biologist. 2:1990;1091-1100.
9. Cirelli C., Pompeiano M., Tononi G. In vivo antisense approaches to the role of immediate early gene expression in the brain. Regul. Pept. 59:1995;151-162.
10. Cossum P.A., Sasmor H., Dellinger D., Truong L., Cummins L., Owens S.R., Markham P.M., Shea J.P., Crooke S. Disposition of the C-14-labeled phosphorothioate oligonucleotide ISIS 2105 after intravenous administration to rats. Behavior Genetics. 26:1993;279-292.
11. Fisher T.L., Terhorst T., Cao X., Wagner R.W. Intracellular disposition and metabolism of fluorescently labeled unmodified and modified oligonucleotides microinjected into mammalian cells. Nucleic Acids Res. 21:1993;3857-3865.
12. Graybiel A.M., Moratalla R., Robertson H.A. Amphetamine and cocaine induce drug specific activation of the c-fos gene in striosome-matrix compartments and limbic subdivisions of the striatum. Proc. Natl. Acad. Sci. U.S.A. 87:1990;6912-6916.
13. P.S. Ho, V. Livanov, W. Zhang, J.-H. Li, T. Lesher, Modification of phosphorothioate oligonucleotides yields potent analogs with minimal toxicity for antisense experiments in the CNS, submitted.
14. Hooper M.L., Chiasson B.J., Robertson H.A. Infusion into the brain of an antisense oligonucleotide to the immediate-early gene c-fos suppresses production of Fos and produces a behavioral effect. Neuroscience. 63:1994;917-924.
15. Konradi C., Cole R.L., Heckers S., Hyman S.E. Amphetamine regulates gene expression in rat striatum via transcription factor, CREB. J. Neurosci. 14:1994;5623-5634.
16. McCarthy M.M., Brooks P.J., Pfaus J.G., Brown H.E., Flanagan L.M., Schwartz-Giblin S., Pfaff D.W. Antisense oligodeoxynucleotides in behavioral neuroscience. Neuroprotocols. 2:1993;67-74.
17. M.-M. Mesulam, Principles of horseradish peroxidase neurohistochemistry and their application for tracing neural pathways - axonal transport, enzyme histochemistry and light microscopic analysis, in: M.-M. Mesulam (Ed.), Tracing Neural Connections with Horseradish Peroxidase, Wiley, Chichester, 1982, pp. 1-151.
18. Morgan J.I., Curran T. Stimulus-transcription coupling in the nervous system: involvement of the inducible protooncogenes fos and jun. Ann. Neurosci. 14:1991;421-451.
19. Ogawa S., Pfaff D.W. Application of antisense DNA method for the study of molecular bases of brain function and behavior. Behavior Genetics. 26:1996;279-292.
20. Ogawa S., Brown H.E., Okano H.J., Pfaff D.W. Cellular uptake of intracerebrally administered oligodeoxynucleotides in mouse brain. Regul. Pept. 59:1995;143-149.
21. Ogawa S., Olazabai U.E., Parhar I.S., Pfaff D.W. Effects of intrahypothalamic administration of antisense DNA for progesterone receptor mRNA on reproductive behavior and progesterone receptor immunoreactivity in female rat. J. Neurosci. 14:1994;1766-1774.
22. G. Paxinos, C. Watson, The rat brain in stereotaxic coordinates, 2nd edn., Academic Press, Sydney, 1986.
23. K.-H. Schlingensiepen, M. Heilig, Gene function analysis and therapeutic prospects in neurobiology, in: R. Schlingensiepen, W. Brysch, K.-H. Schliengensiepen (Eds.), Antisense - From Technology to Therapy, Blackwell, Berlin, 1997, pp. 186-223.
24. Skagerberg G., Björklund A., Lindvall O. Further studies on the use of the fluorescent retrograde tracer, True Blue, in combination with monoamine histochemistry. J. Neurosci. Methods. 14:1985;25-40.
25. Sommer W., Bjelke B., Ganten D., Fuxe K. Antisense oligonucleotide to c-fos induces ipsilateral rotational behavior to D-amphetamine. NeuroReport. 5:1993;277-280.
26. Sommer W., Rimondini R., O'Connor W., Hansson A.C., Ungerstedt U., Fuxe K. Intrastriatally injected c-fos antisense oligonucleotide interferes with striatonigral but not striatopallidal - aminobutyric acid transmission in the conscious rat. Proc. Natl. Acad. Sci. U.S.A. 93:1996;14134-14139.
27. Szklarczyk A., Kaczmarek L. Antisense oligodeoxyribonucleotides: stability and distribution after intracerebral injection into the rat brain. J. Neurosci. Methods. 60:1995;181-187.
28. Vanegas H., Holländer H., Diestel H. Early stages of uptake and transport of horseradish peroxidase by cortical structures and its use for the study of local neurons and their processes. J. Comp. Neurol. 177:1978;193-212.
29. Wahlestedt C. Antisense oligonucleotide strategies in neuropharmacology. Trends Pharmacol. Sci. 15:1994;42-46.
30. Wahlestedt C., Pich E.M., Koob G.F., Yee F., Heilig M. Modulation of anxiety and neuropeptide Y-Y1 receptors by antisense oligonucleotides. Science. 259:1993;528-531.
31. Whitesell L., Geselowitz D., Chavany C., Fahmy B., Walbridge S., Alger J.R., Neckers L.M. Stability, clearance, and disposition of intraventricularly administered oligodeoxynucleotides: implications for therapeutic application within the central nervous system. Proc. Natl. Acad. Sci. U.S.A. 90:1993;4665-4669.
32. Yaida Y., Nowak T.S. Jr. Distribution of phosphodiester and phosphorothioate oligonucleotides in rat brain after interventricular and intrahippocampal administration determined by in situ hybridization. Regul. Pept. 59:1995;193-199.
33. Yaida Y., Pulsinelly W.A., Nowak T.S. Jr. Phosphorothioate oligonucleotides as in vivo antisense reagents in brain. Distribution, stability and toxicity. J. Cereb. Blood Flow Metab. 15 1:1995;S54. Suppl.
34. Yee F., Ericson H., Reis D.J., Wahlestedt C. Cellular uptake of intracerebroventricularly administered biotin- or digoxigenin-labeled antisense oligodeoxynucleotides in the rat. Cell. Mol. Neurobiol. 14:1994;475-486.
35. Zhang S.-P., Zhou L.-W., Morabito M., Lin R.C.S., Weiss B. Uptake and distribution of fluorescein-labeled D2 dopamine receptor antisense oligonucleotide in mouse brain. J. Mol. Neurosci. 7:1996;13-28.