Cloning of a thermostable ascorbate oxidase gene from Acremonium sp. HI-25 and modification of the azide sensitivity of the enzyme by site-directed mutagenesis

Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology

Volumn 1388, Issue 2, 1998, Pages 444-456

  Takeda, Kayoko ^a,c   Itoh, Homare ^a   Yoshioka, Issei ^b   Yamamoto, Megumi ^b   Misaki, Hideo ^b   Kajita, Sachiko ^c   Shirai, Kengo ^c   Kato, Masashi ^c   Shin, Takashi ^d   Murao, Sawao ^d   Tsukagoshi, Norihiro ^c  

^a Ichibiki Co Ltd   (Japan)

^b ASAHI CHEMICAL INDUSTRY CO   (Japan)

^c NAGOYA UNIVERSITY   (Japan)

^d SOJO UNIVERSITY   (Japan)

Author keywords

Azide resistance; Expression in Aspergillus nidulans; Fungal ascorbate oxidase gene; Site directed mutagenesis; Trinuclear copper site

Indexed keywords

ASCORBATE OXIDASE; AZIDE; RECOMBINANT ENZYME; SIGNAL PEPTIDE;

ACREMONIUM; ARTICLE; ASPERGILLUS NIDULANS; FUNGAL GENETICS; GENE SEQUENCE; INTRON; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS;

ACREMONIUM; AMINO ACID SEQUENCE; ASCORBATE OXIDASE; AZIDES; BASE SEQUENCE; BINDING SITES; CLONING, MOLECULAR; CONSERVED SEQUENCE; COPPER; ENZYME STABILITY; FUNGAL PROTEINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; RECOMBINANT PROTEINS; RESTRICTION MAPPING; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; TEMPERATURE;

ACREMONIUM SP.; ACREMONIUM SP. HI-25; ASPERGILLUS; ASPERGILLUS ORYZAE; EMERICELLA NIDULANS;

EID: 0032506284     PISSN: 01674838     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-4838(98)00206-4     Document Type: Article

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