Cytokine transgene expression and promoter usage in primary CD34+ cells using particle-mediated gene delivery

Human Gene Therapy

Volumn 9, Issue 15, 1998, Pages 2197-2205

  Ye, Zi Qing ^a   Qiu, Ping ^b   Burkholder, Joseph K ^b   Turner, Joel ^b   Gulp, Jerilyn ^b   Roberts, Tim ^b   Shahidi, Nasrollah T ^a   Yang, Ning Sun ^b,c  

^a UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

^b Process Formulation and Analytical Laboratories   (United States)

^c UNIVERSITY OF WISCONSIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; CD34 ANTIGEN; COMPLEMENTARY DNA; CYTOKINE; ELONGATION FACTOR 1ALPHA; GAMMA INTERFERON; GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR; INTERLEUKIN 2; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; BIOLISTIC TRANSFORMATION; CONTROLLED STUDY; DNA TRANSFECTION; GENE EXPRESSION; GENE TARGETING; HUMAN; HUMAN CELL; PROMOTER REGION; REPORTER GENE; TRANSCRIPTION REGULATION; TRANSGENE; UMBILICAL CORD BLOOD;

CYTOMEGALOVIRUS;

EID: 0032505706     PISSN: 10430342     EISSN: None     Source Type: Journal    
DOI: 10.1089/hum.1998.9.15-2197     Document Type: Article

References (6)

1. ACSADI, G., JIAO, S., JANI, A., DUKE, D., WILLIAMS, P., CHONG, W., and WOLFF, J.A. (1991). Direct gene transfer and expression into rat heart in vivo. New Biol. 3, 71-81.
2. BODINE, D.M., MCDONAGH, K.T., BRANDT, S.J., NEY, P.A., AGRICOLA, B., BYRNE, E., and NIENHUIS, A.W. (1990). Development of a high-titer retrovirus producer cell line capable of gene transfer into rhesus monkey hematopoietic stem cells. Proc. Natl. Acad. Sci. U.S.A. 87, 3738-3742.
3. BODINE, D.M., MORITZ, T., DONAHUE, R.E., LUSKEY, B.D., KESSLER, S.W., MARTIN, D.I.K., ORKIN, S.H., NIENHUIS, A.W., and WILLIAMS, D.A. (1993). Long-term in vivo expression of a murine adenosine deaminase gene in rhesus monkey hematopoietic cells of multiple lineages after retroviral mediated gene transfer into CD34+ bone marrow cells. Blood 82, 1975-1980.
4. BURKHOLDER, J.K., DECKER, J., and YANG, N.-S. (1993). Rapid transgene expression in lymphocyte and macrophage primary cultures after particle bombardment-mediated gene transfer. J. Immunol. Methods 165, 149-156.
5. CHENG, L., ZIEGELHOFFER, P.R., and YANG, N.-S. (1993). In vivo promoter activity and transgene expression in mammalian somatic tissues evaluated using particle bombardment. Proc. Natl. Acad. Sci. U.S.A. 90, 4455-4459.
6. CIGNETTI, A., GUARINI, A., CARBONE, A., FORNI, M., CRONIN, K., FORNI, G., GANSBACHER, B., and FOA, R. (1994). Transduction of the IL-2 gene into human acute leukemia cells: Induction of tumor rejection without modifying cell proliferation and IL-2 receptor expression. J. Natl. Cancer Inst. 86, 785-786.